Serum miR-125a-5p and CCL17 Upregulated in Chronic Spontaneous Urticaria and Correlated with Treatment Response

Abstract

Currently, there exist no generally accepted objective biomarkers for chronic spontaneous urticaria. Therefore, the identification of new categories of biomarkers for chronic spontaneous urticaria are urgently needed. To the best of our knowledge, no published information is available regarding serum miRNA as related to chronic spontaneous urticaria. In this study, we provide new and important findings which show that serum expression of miR-125a-5p and CCL17 are significantly upregulated in patients with active chronic spontaneous urticaria and significantly decreased in the remission phase. Moreover, a further upregulation in serum miR-125a-5p expression was observed in refractory chronic spontaneous urticaria cases. These results highlight the significance of miR-125a-5p and CCL17 as potential serum biomarkers for chronic spontaneous urticaria. Chronic spontaneous urticaria (CSU) is a common skin disorder associated with autoimmunity. Micro RNAs (miRNAs) are endogenous noncoding RNA mo lecules reported to be potential biomarkers for some autoimmune diseases. In this study, we investigated the association of miRNAs with CSU. A quantitative PCR (qPCR)based array was generated from sera as obtained from 20 active CSU patients and 20 healthy controls. Upregulated or downregulated miRNAs were validated by reverse transcription qPCR in sera from 59 active CSU patients and 58 healthy controls. The ex pression of miR-125a-5p was significantly upregulated in CSU sera and serum levels of CCL17 were also signi ficantly increased in CSU patients. Serum miR-125a-5p expressions were found to be further upregulated in refractory CSU cases (n = 10). In 12 CSU patients in remission, serum miR125a5p expression and CCL17 levels were significantly decreased as compared with that obtained in active phase patients. These results indicated that miR125a5p and CCL17 can serve as potential serum biomarkers for CSU.

<p>Biomarkers In Chronic Spontaneous Urticaria: Current Targets And Clinical Implications</p>

“…Furthermore, the expression of upregulated or downregulated miRNA was recently investigated in CSU patients and the data were then validated by reverse transcription qPCR. The expression of miR-125a-5p and CCL17 levels were found to be significantly increased in the sera of these patients and significantly decreased in the remission phase of the disease, suggesting a potential use of miR-125a-5p and CCL17 as biomarkers of activity of CSU 108…”

Advanced Biomarkers: Therapeutic and Diagnostic Targets in Urticaria

“…A study on CSU patients' serum via qPCR arrays showed serum miR-125a-5p and CCL17 were significantly increased. Additionally, the serum miR-125a-5p and CCL17 levels of 12 CSU patients in the remission phase were significantly lower than those in the active phase [36]. As the target gene of miR-125a-5p, STAT3 cooperates with CCL17 to participate in the chemokine signaling pathway, thereby activating the inflammatory pathway related to Th2 cells.…”

Serum Detection of Anti-thyroid Peroxidase and Anti-thyroglobulin Antibodies in Chinese Patients With Pemphigus Vulgaris and Pemphigus Foliaceus and Literature Review

“…Demographic, clinical and laboratory information of pemphigus patients were retrospectively collected from medical records. Control serum samples were collected from 65 healthy age- and sex-matched Chinese Han ethnic volunteers as described previously ( 25 ). Pemphigus patients with history of thyroid dysfunction or thyroid surgery, or malignancies were excluded.…”