Serum MIP-1α and IL-8 in septic patients

Fujishima, Seitaro; Sasaki, Jun; Shinozawa, Yotaro; Takuma, Kiyotsugu; Kimura, Hiroaki; Suzuki, M; Kanazawa, Minoru; Hori, Shingo; Aikawa, Naoki

doi:10.1007/bf01709331

Cited by 53 publications

(4 citation statements)

References 26 publications

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“…Several chemokines have been identified as biomarkers for sepsis, such as CXCL8, CXCL13, CCL2, and CCL8 [18,[25][26][27][28]. Here, we show that CXCL13 concentration is elevated in the serum of patients with Fig.…”

Section: Discussionsupporting

confidence: 52%

CXCL13 Is Involved in the Lipopolysaccharide-Induced Hyperpermeability of Umbilical Vein Endothelial Cells

et al. 2020

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Sepsis is a disease that is characterized by a severe systemic inflammatory response to microbial infection and lipopolysaccharide (LPS) and is a well-known inducer of sepsis, as well as endothelial cell hyperpermeability. In the present study, we confirm the elevation of CXC chemokine ligand 13 (CXCL13) in sepsis patients. We also show that LPS exposure increases the release of CXCL13, as well as the mRNA and protein expression of CXCL13 and its receptor, CXC chemokine receptor 5 (CXCR5) in human umbilical vein endothelial cells (HUVECs) in a dose-and time-dependent manner. We also examined the effects of CXCL13 knockdown on LPS-mediated endothelial hyperpermeability and tight junction (TJ) protein expression in HUVECs. Our results show that HUVECs exposed to LPS result in a significant decrease in transendothelial electrical resistance (TER) and TJ protein (Zonula occluden-1, occludin, and claudin-4) expression, and a notable increase in fluorescein isothiocyanate (FITC)-dextran flux and p38 phosphorylation, which was partially reversed by CXCL13 knockdown. Recombinant CXCL13 treatment had a similar effect as LPS exposure, which was attenuated by a p38 inhibitor, SB203580. Moreover, the CXCL13neutralizing antibody significantly increased the survival rate of LPS-induced sepsis mice. Collectively, our results show that CXCL13 plays a key role in LPS-induced endothelium hyperpermeability via regulating p38 signaling and suggests that therapeutically targeting CXCL13 may be beneficial for the treatment of sepsis.

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Section: Discussionsupporting

confidence: 52%

CXCL13 Is Involved in the Lipopolysaccharide-Induced Hyperpermeability of Umbilical Vein Endothelial Cells

et al. 2020

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“… 28 , 29 Previous studies have also observed that IL-8 and MCP-1 have a positive correlation with the SOFA score and were higher in non-survivors within the first 24 hours after admission to the ICU. 30 Our results strongly suggested that MCP-1 and IL-8 acted as key chemokines to induce cytokine storms in the abdomen and serum during the progression of sepsis. Unlike other cytokines, which have broad effects on many cells, chemokines are usually cell-specific.…”

Section: Discussionsupporting

confidence: 52%

Evaluation of Biomarkers from Peritoneal Fluid as Predictors of Severity for Abdominal Sepsis Patients Following Emergency Laparotomy

Zhao¹,

Zhang²,

Deng³

et al. 2023

JIR

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Purpose Intra-abdominal infection is considered the second most common cause of sepsis and results in localized or diffused inflammation of the peritoneum. The main treatment for abdominal sepsis is an emergency laparotomy for source control. However, surgical trauma also causes inflammation, and patients become susceptible to postoperative complications. Therefore, it is necessary to identify biomarkers that can be used to distinguish sepsis from abdominal infection. This prospective study investigated whether cytokine levels in the peritoneum could predict complications and indicate severity of sepsis following emergency laparotomy. Methods We prospectively observed 97 patients with abdominal infection admitted to the Intensive Care Unit (ICU). After emergency laparotomy,SEPSIS-3 criteria were used for the diagnosis of sepsis or septic shock. Blood and peritoneal fluid samples were drawn at postoperative admission to the ICU and cytokine concentrations were measured by flow cytometry. Results Fifty-eight postoperative patients were enrolled. We found significant elevations in the peritoneal concentrations of IL-1β, IL-6, TNF-α, IL-17, and IL-2 in patients with sepsis or septic shock compared to the patients without sepsis after surgery. Positive correlations between levels of these peritoneal cytokines with APACHE II scores were found: IL-6, in particular, had the highest correlation coefficient of 0.833. Meanwhile, IL-10 in blood, MCP-1 and IL-8 in both blood and peritoneum were simultaneously increased in patients with sepsis and septic shock, and also positively correlated with disease severity. Conclusion The cytokine storm that occurs in the abdominal cavity after emergency laparotomy may be the main mechanism leading to sepsis. It may be valuable to measure IL-1β, IL-6, TNF-α,IL-17, IL-2, MCP-1, and IL-8 in the peritoneal fluid, combined with serum IL-10, MCP-1 and IL-8, in a panel of cytokines, to assess the severity of sepsis and predict mortality from abdominal infection after emergency laparotomy.

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“…Our animal experiment dealt not only with the therapeutic effect on hypotension but also with treatment prognosis and biomarkers that re ect treatment prognosis. Several studies have con rmed that IL-6 and IL-8 can be used to evaluate the prognosis of sepsis or septic shock [16][17][18] , with the poor prognosis group showing higher concentrations. On the basis of this conclusion, we measured the IL-6 and IL-8 levels of the minipig septic shock model under IO and CVC access for drug administration to indirectly re ect the prognosis for the two routes of norepinephrine administration during septic shock.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of intraosseous norepinephrine infusion in a minipig model of septic shock

Liu

Wang

et al. 2022

Preprint

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Background Studies have indicated that not every drug can achieve the same therapeutic effect or prognosis as intravenous injection when administered via intraosseous (IO) access. The purpose of this study was to establish an experimental minipig model of septic shock to compare the differences in the efficacy for the treatment of hypotension and survival time of IO and central venous catheter (CVC) infusions of norepinephrine. Methods Experimental minipigs were chosen for this study and were randomly divided into IO and CVC groups (n = 4 for each group). Cecal ligation and puncture was performed to establish the septic shock model. Immediately after the septic shock model was established, the IO group received norepinephrine via tibial IO infusion, whereas the CVC group received norepinephrine via internal jugular vein. The vital signs, blood pressure recovery time, post-septic shock survival time and total survival time were recorded. Results There was no significant difference in blood pressure recovery time between IO and CVC groups [40 (20–60) vs 30 (20–60) minutes, P = 0.686]. The cumulative norepinephrine dose during the period of blood pressure recovery time did not differ significantly between IO and CVC groups [10.0 (3.0–21.0) vs 6.5 (3.0–21.0) µg/Kg, P = 0.686]. There were no significant differences in the post-septic shock survival time [7.75 (6.50–10.00) vs 8.75 (6.50–14.00) hours, P = 0.686] and total survival time [21.25 (16.50–35.50) vs 25.75 (19.50–26.50) hours, P = 0.686] between IO and CVC groups. Conclusion These results suggest that tibial IO and CVC infusion of norepinephrine achieved same therapeutic effect on hypotension and survival time.

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Serum MIP-1α and IL-8 in septic patients

Abstract: The production of MIP-1 alpha and IL-8 was increased in sepsis. Furthermore, an initially detectable level of circulating IL-8, but not MIP-1 alpha, predicted a high mortality in sepsis diagnosed according to the ACCP/SCCM criteria.

Cited by 53 publications

References 26 publications

CXCL13 Is Involved in the Lipopolysaccharide-Induced Hyperpermeability of Umbilical Vein Endothelial Cells

CXCL13 Is Involved in the Lipopolysaccharide-Induced Hyperpermeability of Umbilical Vein Endothelial Cells

Evaluation of Biomarkers from Peritoneal Fluid as Predictors of Severity for Abdominal Sepsis Patients Following Emergency Laparotomy

Therapeutic effect of intraosseous norepinephrine infusion in a minipig model of septic shock

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