Serum microRNA panels predict bariatric surgery outcomes

Abstract

Objective:The weight losses after bariatric surgery are modulated by multiple factors in people with obesity. MicroRNAs (miRNAs) have been reported to show significant regulatory roles in adipose tissue. However, a serum miRNA signature to serve as a biomarker of sustained weight losses following bariatric surgery has not yet been established. Methods:MiRNA microarray was used to identify differentially expressed miRNAs in the serum of patients with an effective response after bariatric surgery compared with those without. Excess weight loss > 55% at 6 months after surgery was defined as an effective response.Results: Three miRNAs were shown to have a significantly differential expression between patients with or without an effective response following bariatric surgery. The miR-31-5p was downregulated, whereas miR-328-3p and miR-181a-5p were upregulated in the patients with effective responses compared with those without effective responses. Panels of the serum ratios of miR-328-3p/miR-31-5p or miR-181a-5p/ miR-31-5p and individual BMI value exhibited good performance in preoperative prediction of treatment effectiveness. Bioinformatic analysis depicted that predicted targets of these miRNAs were involved in the regulation of the AMP-activated protein kinase signaling pathway. Conclusions:A circulating miRNA signature with clinical variables (BMI) can be a clinical biomarker to predict effectiveness following bariatric surgery.

Expression and Relations of Unique miRNAs Investigated in Metabolic Bariatric Surgery: A Systematic Review

“…Instead, Nunez identified the most influential miRNA 328-3p, 181a-5p, and 31-5p. Interestingly, 328-3p and 181a-5p miRNAs were only used in Nunez and Yeh et al [9,22] was not identified by other studies as the most pronounced, and 31-5p was in three studies, the most prominent [9,12,22]. Kuryłowicz et al found an effect whereby 122-5p was involved in inflammation and immune response with no further explanation [32].…”

“…In our analysis, miRNA 122 and 122-5p were mentioned the most in the reviewed studies. Specifically, miRNA 122 was mentioned in three distinct studies [4,19,26], while miRNA-122-5p was referenced in seven [9,13,16,17,22,25,32] Blum et al delved into the effects of MBS on vascular endothelial cells (VAC). Their research was geared towards understanding the potential role of MBS in preventing cancer and protecting VAC [26].…”

“…Yeh et al tested serum microRNA panels and found no significant effect on 122-5p in the algorithm to identify circulating miRNAs after MBS (most influenced effect were 877-5p, 1285-3p, 328-3p, 181a-5p, 3688-3p, 31-5p) [9]. Lastly, Yo Nunez et al [22] did mention miRNA 122-5p in the study; they pointed out that it was not among the top three miRNAs that exhibited the most pronounced effects post-weight loss.…”

Towards precision medicine in bariatric surgery prescription

“…The miR-31-5p was downregulated, while miR-328-3p and miR-181a-5p were upregulated in patients with a %EWL > 55% at 6 months after surgery, when compared with those that failed to achieve optimal weight reduction. Panels using the ratios of circulating miR-328-3p/miR-31-5p or miR-181a-5p/miR-31-5p showed a fair performance in the prediction of RYGB efficacy, with an AUC of 0.680 and 0.669, respectively [ 175 ].…”

Molecular, Metabolic, and Nutritional Changes after Metabolic Surgery in Obese Diabetic Patients (MoMen): A Protocol for a Multicenter Prospective Cohort Study

“…Although metabolic surgery is common in developed countries, it has only started to gain recognition in Malaysia over the past few years. The serum miRNA biomarkers of sustained weight loss following metabolic surgery are not yet well-established [ 16 ]. It will be exciting to study these potential biomarkers in our population.…”