Serum microRNA let-7a-1/let-7d/let-7f and miRNA 143/145 Gene Expression Profiles as Potential Biomarkers in HCV Induced Hepatocellular Carcinoma

Aly, Doaa Mamdouh; Gohar, Nadida A.; El-Hady, Afaf Ahmed Abd; Khairy, Mohamed M.; Abdullatif, Mona

doi:10.31557/apjcp.2020.21.2.555

Cited by 20 publications

(13 citation statements)

References 21 publications

(18 reference statements)

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“…miR-143 expression was found down-regulated in the serum of patients with HCV-related HCC than in healthy control group [ 68 ]. Furthermore, miR-143 serum levels were significantly lower in non-cirrhotic patients with CHC when compared to healthy controls [ 68 ]. Then, there is a positive correlation between miR-143 serum levels and fibrosis stages [ 69 ].…”

Section: Mirnas Expression During Chb and Chc Related Fibrosismentioning

confidence: 99%

miRNAs as Potential Biomarkers for Viral Hepatitis B and C

Loureiro

Tout

Narguet

et al. 2020

Viruses

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Around 257 million people are living with hepatitis B virus (HBV) chronic infection and 71 million with hepatitis C virus (HCV) chronic infection. Both HBV and HCV infections can lead to liver complications such as cirrhosis and hepatocellular carcinoma (HCC). To take care of these chronically infected patients, one strategy is to diagnose the early stage of fibrosis in order to treat them as soon as possible to decrease the risk of HCC development. microRNAs (or miRNAs) are small non-coding RNAs which regulate many cellular processes in metazoans. Their expressions were frequently modulated by up- or down-regulation during fibrosis progression. In the serum of patients with HBV chronic infection (CHB), miR-122 and miR-185 expressions are increased, while miR-29, -143, -21 and miR-223 expressions are decreased during fibrosis progression. In the serum of patients with HCV chronic infection (CHC), miR-143 and miR-223 expressions are increased, while miR-122 expression is decreased during fibrosis progression. This review aims to summarize current knowledge of principal miRNAs modulation involved in fibrosis progression during chronic hepatitis B/C infections. Furthermore, we also discuss the potential use of miRNAs as non-invasive biomarkers to diagnose fibrosis with the intention of prioritizing patients with advanced fibrosis for treatment and surveillance.

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Section: Mirnas Expression During Chb and Chc Related Fibrosismentioning

confidence: 99%

miRNAs as Potential Biomarkers for Viral Hepatitis B and C

Loureiro

Tout

Narguet

et al. 2020

Viruses

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“…A recent study has suggested that let-7a was a target for inducing the apoptosis of lung cancer cells [ 9 ]. The serum level of let-7a is suggested as the biomarker for hepatocellular cancer [ 10 ]. Besides, Let-7a was found to be downregulated in scleroderma and contributed to the accumulation of type I collagen [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Let-7a Targeting TNFAPI3 Promotes Vascular Endothelial Cell Apoptosis of Pediatric Patients with Henoch–Schönlein Purpura via NF-κB Signaling Pathway

Cui

Liu

Geng

et al. 2022

Journal of Healthcare Engineering

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Objective. We aimed at exploring the role of let-7a in the pathogenesis of pediatric Henoch–Schönlein purpura (HSP) and its related mechanism. Methods. Fifty-five pediatric HSP patients and 20 paired healthy controls were included. The expressions of let-7a and TNFAIP3 were detected by RT-qPCR or/and western blot. Vessel fibrinoid necrosis was evaluated in skin tissues by PTAH staining. The serum IgA level was measured by ELISA. Cells were transfected with let-7a inhibitor and/or TNFAIP3 siRNA, accompanied by pretreatment with NF-κB inhibitor PDTC or not. After being cultured in HSP serum, the changes in cell viability, cell apoptosis, apoptosis-related proteins, and NF-κB pathway-related proteins were detected by CCK8, flow cytometry, and western blot. Results. The let-7a expression level was positively correlated with the serum IgA level and severity degree of vascular fibrinoid necrosis in HSP patients. Let-7a expression was significantly increased, whereas cell viability and TNFAIP3 expression were obviously decreased 48 h after HUVECs were incubated with HSP serum. Let-7a knockdown upregulated the cell viability, whereas it reduced the apoptotic ratio, apoptosis protein expressions (Bax/Bcl2 ratio, cleaved-caspase 3), and NF-κB pathway activation (reflected by reduced P65 nuclear translocation and p-IκBα expression) in HUVECs (all p < 0.05 ). The changes induced by let-7a knockdown were obviously reversed by TNFAIP3 siRNA transfection p < 0.05 . Besides, PDTC treatment remarkably diminished the anti-apoptosis effect of let-7a knockdown and pro-apoptosis effect of TNFAIP3 siRNA on HUVECs induced by HSP serum. Conclusions. Let-7a knockdown significantly suppressed vascular endothelial cell apoptosis induced by HSP serum by targeting TNFAPI3 via NF-κB signaling pathway. Our findings provided a potential therapeutic target for the treatment of HSP.

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“…In another approach, a fluorescence-based system was devised for microRNA detection with potential for the detection of single nucleotide polymorphisms in clinical and biomedical research . In this work, miRNA let-7, a potential marker for stroke, cardiomyopathy, traumatic brain injury, and various cancers, , was used as an analyte in a system that combined a peroxidase-mimicking DNAzyme with an RNA-cleaving DNAzyme. In this method, thiolated DNA probes, with a recognition site for a Mg 2+ -dependent RNA-cleaving DNAzyme and a region capable of complexing with K + and hemin to form the peroxidase-mimicking DNAzyme, were immobilized onto a Au-coated electrode.…”

Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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Since their discovery almost three decades ago, DNAzymes have been used extensively in biosensing. Depending on the type of DNAzyme being used, these functional oligonucleotides can act as molecular recognition elements within biosensors, offering high specificity to their target analyte, or as reporters capable of transducing a detectable signal. Several parameters need to be considered when designing a DNAzyme-based biosensor. In particular, given that many of these biosensors immobilize DNAzymes onto a sensing surface, selecting an appropriate immobilization strategy is vital. Suboptimal immobilization can result in both DNAzyme detachment and poor accessibility toward the target, leading to low sensing accuracy and sensitivity. Various approaches have been employed for DNAzyme immobilization within biosensors, ranging from amine and thiol-based covalent attachment to non-covalent strategies involving biotin–streptavidin interactions, DNA hybridization, electrostatic interactions, and physical entrapment. While the properties of each strategy inform its applicability within a proposed sensor, the selection of an appropriate strategy is largely dependent on the desired application. This is especially true given the diverse use of DNAzyme-based biosensors for the detection of pathogens, metal ions, and clinical biomarkers. In an effort to make the development of such sensors easier to navigate, this paper provides a comprehensive review of existing immobilization strategies, with a focus on their respective advantages, drawbacks, and optimal conditions for use. Next, common applications of existing DNAzyme-based biosensors are discussed. Last, emerging and future trends in the development of DNAzyme-based biosensors are discussed, and gaps in existing research worthy of exploration are identified.

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Serum microRNA let-7a-1/let-7d/let-7f and miRNA 143/145 Gene Expression Profiles as Potential Biomarkers in HCV Induced Hepatocellular Carcinoma

Cited by 20 publications

References 21 publications

miRNAs as Potential Biomarkers for Viral Hepatitis B and C

miRNAs as Potential Biomarkers for Viral Hepatitis B and C

Let-7a Targeting TNFAPI3 Promotes Vascular Endothelial Cell Apoptosis of Pediatric Patients with Henoch–Schönlein Purpura via NF-κB Signaling Pathway

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

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