Serum microRNA array analysis identifies miR-140-3p, miR-33b-3p and miR-671-3p as potential osteoarthritis biomarkers involved in metabolic processes

Ntoumou, Eleni; Tzetis, Maria; Braoudaki, Maria; Lambrou, George Ι.; Poulou, Myrto; Malizos, Konstantinos N.; Stefanou, Nikolaos; Anastasopoulou, Lydia; Tsezou, Aspasia

doi:10.1186/s13148-017-0428-1

Cited by 127 publications

(108 citation statements)

References 61 publications

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“…A recent screen of 2,549 miRs revealed that miR-140-3p, miR-671-3p, and miR-33b-3p are downregulated in the serum of OA patients compared with healthy individuals, with miR-140-3p and miR-671-3p also downregulated in OA articular cartilage compared with healthy cartilage (137). These results complement previous studies showing reduced miR-140 in OA cartilage and knee synovial fluid (26,43,44,138).…”

Section: Mirs As Biomarkerssupporting

confidence: 84%

The complex landscape of microRNAs in articular cartilage: biology, pathology, and therapeutic targets

et al. 2018

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The disabling degenerative disease osteoarthritis (OA) is prevalent among the global population. Articular cartilage degeneration is a central feature of OA; therefore, a better understanding of the mechanisms that maintain cartilage homeostasis is vital for developing effective therapeutic interventions. MicroRNAs (miRs) modulate cell signaling pathways and various processes in articular cartilage via posttranscriptional repression of target genes. As dysregulated miRs frequently alter the homeostasis of articular cartilage, modulating select miRs presents a potential therapeutic opportunity for OA. Here, we review key miRs that have been shown to modulate cartilage-protective or -destructive mechanisms and signaling pathways. Additionally, we use an integrative computational biology approach to provide insight into predicted miR gene targets that may contribute to OA pathogenesis, and highlight the complexity of miR signaling in OA by generating both unique and overlapping gene targets of miRs that mediate protective or destructive effects. Early OA detection would enable effective prevention; thus, miRs are being explored as diagnostic biomarkers. We discuss these ongoing efforts and the applicability of miR mimics and antisense inhibitors as potential OA therapeutics.

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Section: Mirs As Biomarkerssupporting

confidence: 84%

The complex landscape of microRNAs in articular cartilage: biology, pathology, and therapeutic targets

et al. 2018

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“…Based on bioinformatics and proteomic analysis, Dimitrios et al indicated that the collaborative networks of miRNAs and proteins were associated with OA pathogenesis [40]. In previous studies, serum miRNA array analysis showed that miR-33b-3p may be used as a potential biomarker for OA [41]. Meanwhile, miR-940 is involved in the inflammatory response of chondrocytes in OA [42].…”

Section: Discussionmentioning

confidence: 99%

“…Gene expression profile data in the dataset GSE105027 [18] were downloaded from Gene Expression Omnibus (GEO) database. This dataset was produced on GPL21575 Agilent-070156 Human_miRNA_V21.0_ Microarray 046064 platform.…”

Section: Data Resource and Preprocessingmentioning

confidence: 99%

“…The bioinformatics analysis of gene expression profiles provides new opportunities to uncover the potential OA-related genes such as cathepsin H (CTSH) and cathepsin S (CTSS) [14][15][16][17]. In order to explore the potential differentially expressed miRNAs (DEMs) as a novel breakthrough in the clinical treatment of OA, Ntoumou et al identified a circulating miRNA signature for OA patients using DEMs analysis and related pathway investigation [18]. They found that miRNAs such as miR-140-3p regulated the metabolic processes of OA patients.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-1202 plays a vital role in osteoarthritis via KCNQ1OT1 has-miR-1202-ETS1 regulatory pathway

Liu

Gao

et al. 2020

J Orthop Surg Res

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Background: This study aimed to explore the molecular mechanism of osteoarthritis (OA) and provide information about new genes as potential targets for OA treatment. Methods: Gene expression profile of GSE105027, including 12 OA serum samples (OA group) and 12 healthy serum samples (ctrl group), was downloaded. The differentially expressed miRNAs (DEMs) as well as miRNA-mRNAs interactions were investigated, followed by function and pathway investigation. Then the protein-protein interaction (PPI) network was performed. Furthermore, the long non-coding RNA (lncRNA)-miRNA-mRNA interactions (competing endogenous RNAs, ceRNAs) were investigated. Results: A total of 17 downregulated miRNAs were revealed between OA and ctrl groups. These DEMs such as has-miR-1202 were mainly enriched in GO functions like histone acetyltransferase binding and KEGG pathways like cellular senescence. The integrated PPI network analysis showed that has-miR-1202, has-miR-33b-3p, has-miR-940, has-miR-4284, and has-miR-4281 were 5 downregulated miRNAs in this network. Furthermore, the lncRNA-miRNA-mRNA interactions such as KCNQ1OT1-has-miR-1202-ETS1 were revealed in the present ceRNA network. Conclusion: Key DEMs such as miR-33b-3p, miR-940, and miR-1202 may be involved in OA. miR-1202 may regulate OA development via histone acetyltransferase pathway binding function and cellular senescence pathway. Furthermore, KCNQ1OT1-has-miR-1202-ETS1 might be vital for the process of OA.

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“…This study confirms a previous report of increased miR-155 in OA PBMCs 31 . Ntoumou and colleagues performed a large microarray analysis of OA patient serum 32 . They found a number of miRNAs differentially expressed, including seven which were further validated as biomarkers (using qPCR analysis), each manifesting a receiver operator characteristic area under the curve (ROC-AUC)>0.8.…”

Section: Epigenetics: Noncoding Rnasmentioning

confidence: 99%

Osteoarthritis year in review 2018: genetics and epigenetics

Jeffries

2019

Osteoarthritis and Cartilage

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Objective: This review was designed to identify highlights of the osteoarthritis (OA) genetics and epigenetics literature published between April 2017 and January 2018. Design: A Pubmed literature search was conducted using the keywords 'osteoarthritis' and each of the following: 'genomic', 'genetic', 'epigenomic', 'epigenetic', 'histone', 'noncoding RNA', 'miRNA', 'lncRNA', 'DNA methylation', 'DNA hydroxymethylation', 'DNMT', and 'TET'. The dates of publication were restricted to 4/1/2017e1/15/2018. Results were compared to the same search terms limited to 4/1/2016e1/15/2017. Results: Virtually all search term combinations demonstrated a decrease in papers published this year compared to last, with epigenetic and miRNA/lncRNA research being stable. Despite this, numerous advances were made this year, including the second large genome-wide association study (GWAS) study of hand OA, a new twin study of hip and knee OA concordance, an extensive study of GDF5 evolution, analyses of the contribution of Dnmt3a to OA, a description of DNA methylation in a nonhuman primate model of OA, and an integrated, multi-omics analysis of DNA methylation, mRNA, and protein expression in human OA samples, among others. A variety of micro-and a few circular-RNA studies were also published, highlighting the importance of noncoding RNA in both the pathogenesis and potential treatment of OA. Conclusion: Although publications have decreased slightly in the last year, genetics and epigenetics continue to be a topic of substantial research in OA, and considerable progress continues to be made in the field.

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Serum microRNA array analysis identifies miR-140-3p, miR-33b-3p and miR-671-3p as potential osteoarthritis biomarkers involved in metabolic processes

Cited by 127 publications

References 61 publications

The complex landscape of microRNAs in articular cartilage: biology, pathology, and therapeutic targets

The complex landscape of microRNAs in articular cartilage: biology, pathology, and therapeutic targets

MicroRNA-1202 plays a vital role in osteoarthritis via KCNQ1OT1 has-miR-1202-ETS1 regulatory pathway

Osteoarthritis year in review 2018: genetics and epigenetics

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