Serum Metabolomics Study and Eicosanoid Analysis of Childhood Atopic Dermatitis Based on Liquid Chromatography–Mass Spectrometry

Huang, Yan; Chen, Guoyou; Liu, Xinyu; Shao, Yaping; Gao, Peng; Xin, Chenchen; Cui, Zhen-Ze; Zhao, Xinjie; Xu, Guowang

doi:10.1021/pr5007069

Cited by 58 publications

(76 citation statements)

References 57 publications

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“…A third study by Huang et al , using ultra high performance liquid chromatography tandem mass-spectrometry, reported the reduction of concentration of glycine and taurine conjugated bile acids while the levels of cholic acid and chenodeoxycholic acid were increased in children’s serum suffering from AD. In addition, the increase of free fatty acids was also noted in the study [ 9 ]. Overall it can be pointed out that the work done in the field of metabolomics on atopic dermatitis is still in its beginning phase and a lot of the results need further confirmation and explanation.…”

Section: Introductionsupporting

confidence: 54%

Blood serum metabolome of atopic dermatitis: Altered energy cycle and the markers of systemic inflammation

et al. 2017

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Atopic dermatitis is a chronic inflammatory disease which usually starts in the early childhood and ends before adulthood. However up to 3% of adults remain affected by the disease. The onset and course of the disease is influenced by various genetic and environmental factors. Although the immune system has a great effect on the outcome of the disease, metabolic markers can also try to explain the background of atopic dermatitis. In this study we analyzed the serum of patients with atopic dermatitis using both targeted and untargeted metabolomics approaches. We found the most significant changes to be related to phosphatidylcholines, acylcarnitines and their ratios and a cleavage peptide of Fibrinogen A-α. These findings that have not been reported before will further help to understand this complex disease.

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Section: Introductionsupporting

confidence: 54%

Blood serum metabolome of atopic dermatitis: Altered energy cycle and the markers of systemic inflammation

et al. 2017

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“…To date, profiling and quantification of Wakame GC-MS is a widely used method for the analysis of fatty acids [14]. However, LC-MS has been rarely used but its importance for fatty acid elucidation is increasing [15][16][17][18][19][20][21]. Typically, these methods use a targeted multiple reaction monitoring (MRM) approach [22].…”

Section: Introductionmentioning

confidence: 99%

Free fatty acid profiling in marine algae extract by LC‐MS/MS and isolation as well as quantification of the ω‐3 fatty acid hexadeca‐4,7,10,13‐tetraenoic acid

Schlotterbeck

Kolb

Lämmerhofer

2018

J of Separation Science

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Undaria pinnatifida (Wakame) alga contains high amounts of hexadeca‐4Z,7Z,10Z,13Z‐tetraenoic acid which was reported to decrease the efficiency of cisplatin chemotherapeutics. To obtain a fatty acid enriched extract of this ω‐3 poly‐unsaturated fatty acid as an analytical standard, Wakame was used as source material for its extraction. A two‐step extraction protocol consisting of a liquid‐liquid extraction followed by solid‐phase extraction with 3‐aminopropyl silica in accordance to a normal‐phase elution mode was developed. An ultra high performance liquid chromatography with electrospray ionization tandem mass spectrometry method based on sequential windowed acquisition of all theoretical fragment ion mass spectra allowed a simultaneous comprehensive group selective fatty acids profiling in untargeted manner and quantitative analysis of the targeted fatty acid. Hexadeca‐4Z,7Z,10Z,13Z‐tetraenoic acid was identified using high‐resolution product ion spectra. The quantitative method was based on d5‐deuterated hexadeca‐4Z,7Z,10Z,13Z‐tetraenoic acid which was employed as surrogate calibrant. Preliminary method validation was performed by evaluating detection and quantification limits, linear range, intra‐assay and inter‐day precision. Finally, a concentration of 421.2 ± 14.9 ng/mL (4% CV) of hexadeca‐4Z,7Z,10Z,13Z‐tetraenoic acid was determined in the extract which was further used as analytical standard.

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“…Metabolomics is the science that measures the small molecules that shape human metabolism. allergies, asthma, and others (Kang et al, 2015;Huang et al, 2014;Sirotti et al, 2017;Papamichael et al, 2019).…”

Section: Diet and Dietary Patterns Associated With Chronic Diseasesmentioning

confidence: 99%

Anthropogenic Pressure and Lifestyle are the Underlying Cause of Pandemic Chronic Diseases

Tsoukalas¹,

Sarandi²,

Aristides

et al. 2019

Journal of Siberian Federal University. Biology

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Dramatic changes in the environment in recent decades have resulted in low-nutrient food and excessive intake of heavy metals and toxins through air, drinking water, and dietary habits. Non-communicable diseases (NCD), which are responsible for almost 70% of global deaths, are mainly caused by modifiable risk factors. These include behavioral and metabolic risk factors such as unhealthy diet, tobacco smoke and alcohol. Epigenetic factors have high attributable risks of 80% for most NCDs. Xenobiotics such as persistent organic pollutants, food additives and occupational toxicants, together with nutritional deficiencies, contribute to the development of chronic diseases. Precision medicine integrates the individual variability in genes, environment, and lifestyle of each person for the prevention and treatment of disease. Metabolomics, the quantitative and comprehensive evaluation of metabolites, has emerged as a novel and powerful tool in precision medicine. Analysis of metabolites gives precise data on nutritional deficiencies, metabolic imbalances, environmental toxins, and microbiome conditions and uncovers underlying genetic predispositions that can be modified through diet, lifestyle, supplements or medications. Critical signs of systemic dysfunction at the molecular level can be revealed years before clinical symptoms appear. Thus, through the assessment of the overall health status, early detection of diseases and intervention to restore these deficiencies is feasible. Lifestyle interventions to improve longevity and metabolic balance are being discussed

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Serum Metabolomics Study and Eicosanoid Analysis of Childhood Atopic Dermatitis Based on Liquid Chromatography–Mass Spectrometry

Cited by 58 publications

References 57 publications

Blood serum metabolome of atopic dermatitis: Altered energy cycle and the markers of systemic inflammation

Blood serum metabolome of atopic dermatitis: Altered energy cycle and the markers of systemic inflammation

Free fatty acid profiling in marine algae extract by LC‐MS/MS and isolation as well as quantification of the ω‐3 fatty acid hexadeca‐4,7,10,13‐tetraenoic acid

Anthropogenic Pressure and Lifestyle are the Underlying Cause of Pandemic Chronic Diseases

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