Serum metabolomic analysis of the dose-response effect of dietary choline in overweight male cats fed at maintenance energy requirements

Rankovic, Alexandra; Godfrey, Hannah; Grant, Caitlin E; Shoveller, Anna K.; Bakovic, Marica; Kirby, Gordon M.; Verbrugghe, Adronie

doi:10.1371/journal.pone.0280734

Cited by 4 publications

(1 citation statement)

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“…In cats, metabolomic technologies are highly underutilized compared to other species for dietary interventions [7][8][9][10][11][12][13] and disease states [14][15][16], with only one study to the authors' knowledge assessing the serum metabolome in overweight and obese cats [11]. Said study did, however, not have a lean comparison.…”

Section: Introductionmentioning

confidence: 99%

Description of the fasted serum metabolomic signature of lean and obese cats at maintenance and of obese cats under energy restriction

Grant,

Godfrey,

Tal

et al. 2024

PLoS ONE

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This study aimed to investigate the serum metabolomic profile of obese and lean cats as well as obese cats before and after energy restriction for weight loss. Thirty cats, 16 obese (body condition score 8 to 9/9) and 14 lean (body condition score 4 to 5/9), were fed a veterinary weight loss food during a 4-week period of weight maintenance (L-MAINT and O-MAINT). The 16 obese cats were then energy restricted by a 60% energy intake reduction with the same food for a 10-week period (O-RESTRICT). Fasted serum metabolites were measured using nuclear magnetic resonance and direct infusion mass spectrometry after the maintenance period for L-MAINT and O-MAINT cats and after the energy restriction period for O-RESTRICT and compared between groups using a two-sided t-test. Obese cats lost 672 g ± 303 g over the 10-week restriction period, representing a weight loss rate of 0.94 ± 0.28% per week. Glycine, l-alanine, l-histidine, l-glutamine, 2-hydroxybutyrate, isobutryric acid, citric acid, creatine, and methanol were greater in O-RESTRICT compared to O-MAINT. There was a greater concentration of long-chain acylcarnitines in O-RESTRICT compared to both O-MAINT and L-MAINT, and greater total amino acids compared to O-MAINT. Glycerol and 3-hydroxybutyric acid were greater in O-MAINT compared to L-MAINT, as were several lysophosphatidylcholines. Thus, energy restriction resulted in increased dispensable amino acids in feline serum which could indicate alterations in amino acid partitioning. An increase in lipolysis was not evident, though greater circulating acylcarnitines were observed, suggesting that fatty acid oxidation rates may have been greater under calorie restriction. More research is needed to elucidate energy metabolism and substrate utilization, specifically fatty acid oxidation and methyl status, during energy restriction in strict carnivorous cats to optimize weight loss.

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