Serum-Mediated Inhibition of the Interferon-Gamma-Induced HLA-DR Expression on Monocytes in Patient with Psoriasis

Volk, Hans‐Dieter; Koyka, I; Goan, Silvia‐Renate; Grüner, Stefan; Fiebig, Helmut; Sönnichsen, N; Diezel, W

doi:10.1111/1523-1747.ep12455607

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…This finding is in agreement with previously published data by Stanworth et al [7] and Solder et al [8] who showed that rabbit anti-gpl20 antibodies cross-react with synthetic peptides representative of a sequence within the human 227 observed B cell disorders in HIV-infected patients, such as polyclonal B cell activation and unresponsiveness to stimulation with B cell mitogens [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. Anti-IgG anti bodies can cross-link the antigen receptors on B cells, thus leading to polyclonal B cell activation [9].…”

Section: Discussionsupporting

confidence: 92%

“…Although now generally accepted, there is evidence arguing against the infection of CD4+ cells with HIV be ing the sole mechanism for the development of immuno deficiency: depletion of L3T4 lymphocytes (the analogue of human CD4+ lymphocytes) in the mouse does not lead to immunodeficiency [15], The latent disease-free period with a mean of more than 5 years is very long, although the virus needs only days to weeks for replication [16,17], On the other hand, autoimmune phenomena, such as an in crease of anti-CD4+ autoantibodies and autoantibodyproducing CD5+ B cells, are associated with the devel opment of AIDS [18][19][20][21], Viruses have been implicated as triggers for autoim mune diseases in which an antiviral antibody cross-reacts with the host's own tissue [22,23], A retroviral cause was discussed in Sjogren's syndrome, systemic lupus erythe matosus and Graves' disease, because antibodies directed against the pl7 and p24 gag proteins of HIV-1 and HIV-2 were detectable in some patients [24,25] HIV+ hemophilia patients with platelet counts <50,000/pl had significantly higher IgM-anti-F(ab')2Y and IgM-anti-Fc,, activities than patients with platelet counts >50,000/pl. No significant associ ation was found between IgG-anti-F(ab')2Y, IgG-anti-Fc,, and throm bocytopenia.…”

Section: Resultsmentioning

confidence: 99%

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Anti-IgG Autoantibodies in HIV-Infected Hemophilia Patients

Süsal¹,

Lewin²,

Stanworth³

et al. 1992

Vox Sanguinis

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Sera of 76 HIV-negative hemophilia patients, 103 HIV-positive (HIV+) hemophilia patients free of AIDS or AIDS related complex (ARC), and 32 HIV+ hemophilia patients with AIDS/ARC were tested for four different anti-IgG activities. IgG-anti-F(ab')(2γ), IgM-anti-F(ab')(2γ), and IgG-anti-Fcγ serum activities were significantly associated with the clinical stage of HIV infection, whereas IgM-anti-Fcγ was not. IgG-anti-F(ab')(2γ) activity was found to be caused by cross-reaction of anti-HIV antibody with an epitope within the constant CH1 domain of human IgG. HIV+ hemophilia patients with severe thrombocytopenia (<50,000/µI platelet counts) had significantly higher IgM- anti-IgG activity than patients with >50,000/µI platelets. Because anti-IgG antibodies possess immunoregulatory properties, our results may serve as a possible explanation for the frequent B cell disorders encountered in HIV- infected patients.

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Section: Discussionsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Anti-IgG Autoantibodies in HIV-Infected Hemophilia Patients

Süsal¹,

Lewin²,

Stanworth³

et al. 1992

Vox Sanguinis

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“…In an experimental approach, recombinant IFNy has been administered to patients with atopic dermatitis in a double-blind, placebo-controlled trial and found to be statistically significantly superior to the placebo (29). However, psoriatic serum was demonstrated to interfere with the IFNy-mediated induction of HLA-DR expression on cultured monocytes of the same patients (30). IL-2R (CD25) and ICAM-l (CD54) expression on "small" and "large" monocyte subsets in patients with atopic dermatitis was significantly decreased from the levels in normal controls ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Differences between Human Blood Monocyte Subpopulations in Psoriasis and Atopic Dermatitis

Zheng¹,

Mrowietz

1997

The Journal of Dermatology

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Peripheral blood monocytes seem to be of importance in the initiation and maintenance of cutaneous inflammatory disorders such as psoriasis and atopic dermatitis. Functional abnormalities of monocytes have been observed in both diseases. We sought to determine whether these abnormalities are reflected by an altered phenotypic expression of functionally active surface molecules. Peripheral blood monocyte subsets varying in cellular density and cell size from patients with psoriasis and atopic dermatitis were investigated using FACS analysis employing a panel of monoclonal antibodies (CD14, CD16, HLA-DR, HLA-DO, Fc epsilon RII, IL-2R, ICAM-1, CR3). Furthermore, the modulation of expression by interferon-gamma in monocyte subsets from patients was compared to normal controls. The results show that HLA-DR and -DQ expression on monocyte subsets in psoriatic patients was significantly decreased; "large" monocytes expressed significantly less HLA-DR than "small" monocyte subpopulations. Decreased HLA-DR and -DQ expression could be upregulated by incubation of psoriatic monocytes with IFN gamma. In atopic dermatitis, a different phenotype pattern of monocyte subsets was demonstrated: HLA-DR expression and HLA-DQ expression were both decreased in both "large" and "small" monocytes as compared to normal controls. However, there were no significant differences in HLA-DR and HLA-DQ expression between "large" and "small" monocyte subpopulations in atopic dermatitis. Moreover, the ICAM-1 and IL-2R expression of "large" and "small" monocyte subpopulations was significantly decreased in atopic patients from levels in normal controls and psoriatic patients. The altered expression of HLA-DR, -DQ ICAM-1 and IL-2R could be upregulated by incubation of atopic monocytes with IFN gamma. In addition, there was a significant increase in the percentage of monocytes in the differential count of patients with psoriasis or atopic dermatitis. We conclude that the differential phenotype pattern of surface molecules on monocytes in psoriasis and atopic dermatitis may reflect an abnormal monocyte maturation/differentiation state. This may explain the functional abnormalities of monocytes observed in patients with psoriasis and atopic dermatitis.

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CD4 lymphocyte enumeration: Comparison between flow cytometry and enzyme immunoassay

et al. 1995

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Assessment of the CD4 lymphocyte number, currently performed by flow cytometry, is one of the main laboratory tests for establishing progression to acquired immunodeficiency syndrome (AIDS). An enzyme immunoassay has recently been commercialized which can be very useful for counting CD4 cells in laboratories where flow cytometers are not available. In the present study, a comparative evaluation of CD4 positive lymphocytes with flow cytometry and the enzyme assay was made in healthy subjects (N = 30, R = 0.88, P < 0.0001), human immunodeficiency virus (HIV)-infected individuals (N = 80, R = 0.91, P < 0.0001), and patients with autoimmune diseases (N = 28, R = 0.82, P < 0.001) or psoriasis (N = 18, R = 0.76, P = 0.01). A correlation between the two methodologies was not found in psoriatic patients under treatment with cyclosporin A (N = 7, R = 0.05, not significant). Some differences could be found at low CD4 lymphocyte levels since the influence of CD4 antigen eluted from monocytes or soluble CD4 in the whole blood sample could cause overestimation of CD4 cell numbers by the enzyme assay.

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Serum-Mediated Inhibition of the Interferon-Gamma-Induced HLA-DR Expression on Monocytes in Patient with Psoriasis

Cited by 10 publications

References 10 publications

Anti-IgG Autoantibodies in HIV-Infected Hemophilia Patients

Anti-IgG Autoantibodies in HIV-Infected Hemophilia Patients

Phenotypic Differences between Human Blood Monocyte Subpopulations in Psoriasis and Atopic Dermatitis

CD4 lymphocyte enumeration: Comparison between flow cytometry and enzyme immunoassay

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