Serum markers of apoptosis in the early period of heart transplantation

Sánchez‐Lázaro, Ignacio; Almenar‐Bonet, Luis; Romero-Pelechano, Ana; Portolés, Manuel; Martínez‐Dolz, Luis; Roselló‐Lletí, Esther; González-Juanatey, José Ramón; Rivera-Otero, M.; Salvador-Sanz, Antonio

doi:10.3109/1354750x.2012.664168

Cited by 8 publications

(11 citation statements)

References 22 publications

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“…The expression of IGFBP5 can reverse cisplatin resistance of ESCC [26]. On the other hand, CLAP-2, IGF-I and sTNF-R2 all anti-apoptotic proteins [27,28]. IGF-I can act on different apoptosis control points, including Bcl-2 family proteins, caspase inhibitors and death-induced receptor signal transduction [29].…”

Section: Discussionmentioning

confidence: 99%

POLE2 knockdown reduce tumorigenesis in esophageal squamous cells

et al. 2020

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Background: Esophageal squamous cell carcinoma (ESCC) is one of the most frequent malignant tumors originated from digestive system around the world and the treatment was limited by the unclear mechanism. DNA polymerase epsilon 2, accessory subunit (POLE2) is involved in DNA replication, repair, and cell cycle control, whose association with ESCC is still not clear. Methods: In this study, the expression level of POLE2 in ESCC tissues was detected by IHC. The POLE2 knockdown cell line was constructed, identified by qPCR and western blot and used for detecting cellular functions and constructing xenotransplantation mice model. MTT Assay, colony formation assay, flow cytometry, wound-healing assay and Transwell assay were used to detected cell proliferation, apoptosis and migration. Results: We firstly identified that the expression of POLE2 was overexpressed in ESCC. Moreover, the high expression of POLE2 can predict the tumor deterioration and poor prognosis of ESCC patients. Additionally, downregulation of POLE2 was involved in ESCC progression by promoting proliferation, migration, and inhibiting apoptosis in vitro. In vivo studies proved that POLE2 was positively correlated with ESCC tumor formation, which was consistent with the results in vitro. We also illuminated that POLE2 knockdown upregulated pro-apoptotic proteins (Bax, Caspase3, CD40L, FasL, IGFBP-5 and P21) and downregulated anti-apoptotic proteins (CLAP-2, IGF-I and sTNF-R2). In addition, POLE2 was involved in ESCC via targeting PI3K/Akt, Cyclin D1 signaling pathway. Conclusions: Therefore, POLE2 was proved to be involved in the development of ESCC, which may be a potential therapeutic target and bring new breakthroughs in the treatment of ESCC.

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Section: Discussionmentioning

confidence: 99%

POLE2 knockdown reduce tumorigenesis in esophageal squamous cells

et al. 2020

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“…Fortunately, decoy receptor 2 (DcR2), a transmembrane receptor of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), is a cellular senescent marker with an ectodomain proven to be shearable and detectable (3,7,19). DcR2 is detected in both senescent tumor cells and fibroblasts (3,18).…”

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confidence: 99%

Urinary DcR2 is a novel biomarker for tubulointerstitial injury in patients with diabetic nephropathy

Chen

Zhang

Chen

et al. 2017

American Journal of Physiology-Renal Physiology

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Tubulointerstitial injury (TII) plays a crucial role in the progression of diabetic nephropathy (DN), but lack of specific and sensitive biomarkers for monitoring TII in DN management. This study is to investigate whether urinary decoy receptor 2 (uDcR2) could serve as a novel noninvasive biomarker for assessing TII in DN. We recruited 311 type 2 diabetics and 139 DN patients who were diagnosed by renal biopsy. uDcR2 levels were measured by ELISA, and renal DcR2 expression was detected immunohistochemically. Associations between uDcR2 and renal DcR2 and renal functional parameters were evaluated. Receiver operating characteristics (ROC) curve analyzed area under the curve (AUC) of uDcR2 for assessing TII. Double staining was undertaken for renal DcR2 with proximal and distal tubular markers; senescent markers p16, p21, and senescence-associated β-galactosidase (SA-β-gal); and fibrotic markers collagen I and IV. We found DcR2 was primarily expressed in renal proximal tubules; uDcR2 levels were elevated per albuminuria stratum and correlated with renal functional parameters in diabetics and were associated with percentage of tubular DcR2 and TII score in DN. The uDcR2 had an AUC of 0.909 for assessing TII in DN by ROC analysis. Almost all tubular DcR2 was coexpressed with p16 and p21, and nearly more than one-half of tubular DcR2 was positive for SA-β-gal, primarily in collagen I- and IV-positive regions of DN. Our results indicate uDcR2 could potentially serve as a novel biomarker for TII and may reflect senescence of renal proximal tubular cells in DN pathogenesis.

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“…Many researches have reported that after cardiac transplantation myocardial cell showed an apoptosis process [31,32]. In current studies, myocyte apoptosis was detected in allografts, but not in isografts [33].…”

Section: Pkm2 Was Relevant To Cardiomyocytes Apoptosis In Allograftmentioning

confidence: 50%

“…Apoptosis, or "programmed cell death" is implicated in several different heart diseases, and the results observed in terms of the existence of a possible relationship between apoptosis and acute cellular rejection are disparate [33]. In addition, many enunciable studies intensively demonstrated the existence of apoptosis in cardiac tissue samples [32,33,37]. In general, most studies reveal a higher number of apoptosis parameters as the rejection grade increases.…”

Section: Discussionmentioning

confidence: 92%