Serum levels of sICAM-1, sVCAM-1, sE-selectin, sP-selection in healthy Bulgarian people

Abstract

Background: Endothelial dysfunction is increasingly recognized as an important early feature of vascular disease. As the damage to endothelium is a key underlying factor in the development and progression of atherosclerotic processes, markers of endothelial abnormalities have been sought. Increased expression of cell adhesion molecules (CA Ms) on the vascular endothelium has been postulated to play a significant role in atherogenesis. Both in vitro and in vivo studies have suggested that different risk factors of atherosclerosis may increase expression of CA Ms. The elevated level of soluble forms of CA Ms in circulation is associated with a higher risk to future cardiovascular events in subjects predisposed to atherosclerosis. Objective: To determine the reference range for serum concentration of soluble cell adhesion molecules - sICA M-1, sVCA M-1, sE-selectin, sP-selectin. Materia l and methods: We studied 110 healthy people of Bulgarian nationality aged 18-65. The selection criteria for the reference group were made in accordance with the requirements of the International Federation of Clinical Chemistry (IFCC ). Serum concentrations of CA Ms were analysed by means of EL ISA assay. Results: The results are presented as central 95% interval and 0.90 confidence interval of the reference range. Reference ranges were determined for sICA M-1 (128.9 - 347.48 ng/ml), sVCA M-1 (170.42 - 478.36 ng/ml), sE-selectin (9.15 - 65.19 ng/ml) and sP-selectin (101.86 - 209.7 ng/ml). As we found no sex-related differences in the CA Ms concentrations (p > 0.05) there needed to be no separate reference intervals for men and women. The single-factor dispersion analysis we used in analysing the effect of age found no agerelated dependence (p > 0.05, F = 1.038) for the serum CA M concentrations in the 18-65 age range, which means that it is not necessary to establish reference intervals for smaller age ranges in this age group. Conclusion: The reference ranges for sICA M-1, sVCA M-1, sE-selectin, sP-selectin computed in accordance with the results distribution can be used as baseline criteria in clinical laboratory studies.

Increased Soluble VCAM-1 and Normal P-Selectin in Cystic Fibrosis: a Cross-Sectional Study

“…For instance, its serum levels in HS in our study were similar to that found by some other research groups [43, 55, 56]. They are, however, higher than reported in a number of other publications [30, 33, 57]. We suppose that this variability might be due to specificity of antibodies used in ELISA kits.…”

“…Another study, by Romano et al, reported sP-selectin to be higher in 20 CF patients compared with 20 HS [20] and to correlate with worse FEV1%. In our study, sP-selectin levels in patients and HS did not differ and the medians were within the reference range established by Deneva-Koycheva et al (102–210 ng/mL in the serum) [57]. …”

Review of biomarkers to assess the effects of switching from cigarettes to modified risk tobacco products

“…There are differences in sICAM levels in subjects of different ethnic backgrounds with levels being lower in individuals of African origin compared to whites (Hwang et al 1997, Lutsey et al 2006, Miller and Cappuccio 2007. Although plasma ICAM levels fall in children between the ages of 9 and 16 years (Nash et al 1996), recent work suggests that there are no agerelated differences between 23 and 74 years of age (Deneva-Koycheva et al 2011, Bottino et al 2015 which contradicts earlier evidence in which a linear relationship between the plasma concentration of sICAM and age were found in a comparison between subjects <40 (n ¼ 52) and subjects >55 (n ¼ 38) years of age (Miles et al 2001). There is evidence that higher levels of sICAM in older subjects is significantly associated with frailty (Lee et al 2016) and whilst there is no difference between healthy males and females (Deneva-Koycheva et al 2011), sICAM levels are lower in pre-menopausal women than in post-menopausal women (Nyberg et al 2014).…”

Expression of Soluble Intercellular Adhesion Molecule 1 in Colorectal Cancer and Potential Use as Treatment Response Index

“…However, this does not always facilitate timely detection of early recurrence and new metastases. Therefore, there is an increasing need for highly reliable and convenient tools for estimating the was no age-related dependence for serum sICAM-1 concentrations in the 18-65 age range for both sexes in 110 healthy volunteers of the same geographic region [33]. Another limitation was the short observation period because it assessed both the effect of systemic chemotherapy on sICAM-1 and CEA serum levels in patients with advanced CRC after three months of systemic chemotherapy instead of the standard treatment period of six months [34].…”