Serum levels of osteopontin are increased in SIRS and sepsis

Vaschetto, Rosanna; Nicola, Stefania; Olivieri, Carlo; Boggio, Elena; Piccolella, Fabio; Mesturini, Riccardo; Damnotti, Federica; Colombo, Davide; Navalesi, Paolo; Corte, Francesco Della; Dianzani, Umberto; Chiocchetti, Annalisa

doi:10.1007/s00134-008-1268-4

Cited by 60 publications

(51 citation statements)

References 37 publications

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“…In accordance with a recent report revealing elevated circulating levels of OPN in patients with bacterial sepsis predominantly suffering from pneumonia [10], we demonstrate elevated local and systemic levels of OPN during Klebsiella pneumonia in mice. OPN was released rapidly into the bronchoalveolar space upon infection of the airways with K. pneumoniae, where it contributed significantly to the early recruitment of neutrophils.…”

Section: Discussionsupporting

confidence: 93%

Osteopontin promotes host defense during Klebsiella pneumoniae-induced pneumonia

Windt¹,

Hoogerwerf²,

Vos³

et al. 2010

European Respiratory Journal

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Klebsiella pneumoniae is a common cause of nosocomial pneumonia. Osteopontin (OPN) is a phosphorylated glycoprotein involved in inflammatory processes, some of which is mediated by CD44. The aim of this study was to determine the role of OPN during K. pneumoniaeinduced pneumonia.Wild-type (WT) and OPN knockout (KO) mice were intranasally infected with 10 4 colony forming units of K. pneumoniae, or administered Klebsiella lipopolysaccharides (LPS). In addition, recombinant OPN (rOPN) was intranasally administered to WT and CD44 KO mice. During Klebsiella pneumonia, WT mice displayed elevated pulmonary and plasma OPN levels. OPN KO and WT mice showed similar pulmonary bacterial loads 6 h after infection; thereafter, Klebsiella loads were higher in lungs of OPN KO mice and the mortality rate in this group was higher than in WT mice. Early neutrophil recruitment into the bronchoalveolar space was impaired in the absence of OPN after intrapulmonary delivery of either Klebsiella bacteria or Klebsiella LPS. Moreover, rOPN induced neutrophil migration into the bronchoalveolar space, independent from CD44. In vitro, OPN did not affect K. pneumoniae growth or neutrophil function.In conclusion, OPN levels were rapidly increased in the bronchoalveolar space during K. pneumoniae pneumonia, where OPN serves a chemotactic function towards neutrophils, thereby facilitating an effective innate immune response.

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Section: Discussionsupporting

confidence: 93%

Osteopontin promotes host defense during Klebsiella pneumoniae-induced pneumonia

Windt¹,

Hoogerwerf²,

Vos³

et al. 2010

European Respiratory Journal

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“…Based on these pro-inflammatory functions, OPN is therefore considered as a pro-inflammatory molecule and have been well investigated in a wide range of chronic inflammatory diseases (17–20). In contrast to its chronic pathophysiological role, current literatures show inadequate evidence on its role in acute inflammatory diseases, even though a few studies were carried-out in non-sterile inflammatory disease conditions, like polymicrobial sepsis, and Pneumococcal pneumonia infections (28–30). Therefore, dealing with the elucidation of the role of OPN in sterile and acute inflammation will enhance our understanding towards implementing OPN as a novel therapeutic target in a wide range of inflammatory diseases.…”

Section: Discussionmentioning

confidence: 99%

Neutralization of Osteopontin Ameliorates Acute Lung Injury Induced by Intestinal Ischemia-Reperfusion

Hirano

Aziz

Yang

et al. 2016

Shock

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Intestinal ischemia-reperfusion (I/R) is associated with acute respiratory distress syndrome (ARDS). Osteopontin (OPN), a glycoprotein secreted from immune-reactive cells, plays a deleterious role in various inflammatory diseases. Considering OPN as a pro-inflammatory molecule, we hypothesize that the treatment with its neutralizing antibody (anti-OPN Ab) protects mice against intestinal I/R-induced acute lung injury (ALI). Intestinal I/R was induced in mice by superior mesenteric artery (SMA) occlusion with a vascular clip. After 45 minutes of occlusion, the clip was removed and anti-OPN Ab (25 µg/mouse) or normal IgG isotype control (25 µg/mouse) was immediately administrated intravenously. Blood, small intestine, and lung tissues were collected at 4 hours after reperfusion for various analyses. After intestinal I/R, mRNA and protein levels of OPN were significantly induced in the small intestine, lungs, and blood relative to sham-operated animals. Compared with the IgG control group, treatment of anti-OPN Ab significantly reduced plasma levels of pro-inflammatory cytokine and chemokine (IL-6 and MIP-2) and organ injury markers (AST, ALT, and LDH). The histological architecture of the gut and lung tissues in anti-OPN Ab-treated intestinal I/R-induced mice showed significant improvement versus the IgG control mice. The lung inflammation measured by the levels of IL-6, IL-1β, and MIP-2 was also significantly downregulated in the anti-OPN Ab-treated mice as compared with the IgG control mice. Besides, the lung MPO and neutrophil infiltration in anti-OPN Ab-treated mice showed significant reduction as compared with the IgG control animals. In conclusion, we have demonstrated beneficial outcomes of anti-OPN Ab treatment in protecting against ALI, implicating a novel therapeutic potential in intestinal I/R.

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“…Cytokine concentrations on the other hand, showed the highest concentrations 1 day after injury and were lower, but still higher than in healthy reference knees, at later time-points after injury. Since OPN is known to have proinflammatory abilities 17,36,37 and SPARC correlated moderately to levels of pro-inflammatory cytokines in this study, we hypothesize that OPN and SPARC, normally not found in abundance in other tissues than bone, may cause prolonged inflammation in the acutely injured joint. Considering the increasing body of evidence suggesting that inflammation is involved in the OA process, and specifically post-traumatic OA development 34 , we suggest that this hypothesis should be further tested.…”

Section: Table IIImentioning

confidence: 93%

Cartilage and bone markers and inflammatory cytokines are increased in synovial fluid in the acute phase of knee injury (hemarthrosis) – a cross-sectional analysis

Swärd

Frobell

Englund

et al. 2012

Osteoarthritis and Cartilage

141

155

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Serum levels of osteopontin are increased in SIRS and sepsis

Abstract: 45. SIRS/Sepsis: clinical studies.

Cited by 60 publications

References 37 publications

Osteopontin promotes host defense during Klebsiella pneumoniae-induced pneumonia

Osteopontin promotes host defense during Klebsiella pneumoniae-induced pneumonia

Neutralization of Osteopontin Ameliorates Acute Lung Injury Induced by Intestinal Ischemia-Reperfusion

Cartilage and bone markers and inflammatory cytokines are increased in synovial fluid in the acute phase of knee injury (hemarthrosis) – a cross-sectional analysis

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