Serum Levels of KL-6 for Predicting the Occurrence of Radiation Pneumonitis After Stereotactic Radiotherapy for Lung Tumors

Hara, Ryusuke; Itami, Jun; Komiyama, Takafumi; Katoh, Daiki; Kondo, Tatsuya

doi:10.1378/chest.125.1.340

Cited by 58 publications

(31 citation statements)

References 12 publications

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“…Upon epithelial breakdown, KL-6 is thought to leak into the circulation, where it can be measured by a commercially available ELISA kit. KL-6 has been studied extensively in mainly Japanese patients, where serum levels were found to be increased in various interstitial lung diseases such as radiation pneumonitis [22][23][24], CTD-associated lung disease [25][26][27] and drug induced pneumonitis [28]. Interestingly, KL-6 may have a role in fibrosis itself, as it was shown to induce proliferation of lung fibroblasts in vitro [29].…”

Section: Kl-6mentioning

confidence: 99%

Serum biomarkers in idiopathic pulmonary fibrosis

Blink

Wijsenbeek

Hoogsteden

2010

Pulmonary Pharmacology & Therapeutics

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Section: Kl-6mentioning

confidence: 99%

Serum biomarkers in idiopathic pulmonary fibrosis

Blink

Wijsenbeek

Hoogsteden

2010

Pulmonary Pharmacology & Therapeutics

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“…Previous studies have emphasized the importance of this investigation in the field of occupational exposure [1], nuclear accident [2], and for early markers of clinical response to radiation therapy [3][4][5][6]. A report from the International Atomic Energy Agency has recently pointed out the increasing interest in the characterization of molecular profiles that predict normal tissue radiotherapy response [7].…”

Section: Introductionmentioning

confidence: 99%

Time‐course analysis of mouse serum proteome changes following exposure of the skin to ionizing radiation

et al. 2007

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Radiation-induced lesion outcomes of normal tissues are difficult to predict. In particular, radiotherapy or local exposure to a radioactive source by accident can trigger strong injury to the skin. The finding of biomarkers is of fundamental relevance for the prediction of lesion apparition and its evolution, and for the settlement of therapeutic strategies. In order to study radiationinduced cutaneous lesions, we developed a mouse model in which the dorsal skin was selectively exposed to ionizing radiation (IR). 2-D difference gel electrophoresis (2-D DIGE) coupled with MS was used to investigate proteins altered in expression and/or PTM in serum. Proteome changes were monitored from 1 day to 1 month postirradiation, at a dose of 40 Gy, in this specific model developing reproducible clinical symptoms ranging from erythema to skin ulceration with wound healing. About 60 proteins (including some isoforms and likely post-translational variants), representing 20 different proteins, that exhibited significant and reproducible kinetic expression changes, were identified using MS and database searches. Several proteins, down-or up-regulated from day one, could prove to be good candidates to prognosticate the evolution of a skin lesion such as necrosis. In addition, we observed shifts in pI of several spot trains, revealing potential PTM changes, which could also serve as indicators of irradiation or as predictors of lesion severity.

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“…Prior studies have highlighted the importance of this research in the epidemiologic field of occupational exposure (1), exposure assessments in cases of environmental or industrial accidents (2), astronaut exposure in manned space missions (3)(4)(5), and for early markers of clinical response to radiation therapy for cancer (6)(7)(8). Recently, fears of radiological terror incidents have renewed the search for rapid and simple methods to identify exposed individuals within large populations and to predict health effects (9,10).…”

Section: Introductionmentioning

confidence: 99%

Discovering Clinical Biomarkers of Ionizing Radiation Exposure with Serum Proteomic Analysis

et al. 2006

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In this study, we sought to explore the merit of proteomic profiling strategies in patients with cancer before and during radiotherapy in an effort to discover clinical biomarkers of radiation exposure. Patients with a diagnosis of cancer provided informed consent for enrollment on a study permitting the collection of serum immediately before and during a course of radiation therapy. High-resolution surfaceenhanced laser desorption and ionization-time of flight (SELDI-TOF) mass spectrometry (MS) was used to generate high-throughput proteomic profiles of unfractionated serum samples using an immobilized metal ion-affinity chromatography nickel-affinity chip surface. Resultant proteomic profiles were analyzed for unique biomarker signatures using supervised classification techniques. MS-based protein identification was then done on pooled sera in an effort to begin to identify specific protein fragments that are altered with radiation exposure. Sixty-eight patients with a wide range of diagnoses and radiation treatment plans provided serum samples both before and during ionizing radiation exposure. Computer-based analyses of the SELDI protein spectra could distinguish unexposed from radiation-exposed patient samples with 91% to 100% sensitivity and 97% to 100% specificity using various classifier models. The method also showed an ability to distinguish high from low dose-volume levels of exposure with a sensitivity of 83% to 100% and specificity of 91% to 100%. Using direct identity techniques of albuminbound peptides, known to underpin the SELDI-TOF fingerprints, 23 protein fragments/peptides were uniquely detected in the radiation exposure group, including an interleukin-6 precursor protein. The composition of proteins in serum seems to change with ionizing radiation exposure. Proteomic analysis for the discovery of clinical biomarkers of radiation exposure warrants further study. (Cancer Res 2006; 66(3): 1844-50)

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Serum Levels of KL-6 for Predicting the Occurrence of Radiation Pneumonitis After Stereotactic Radiotherapy for Lung Tumors

Cited by 58 publications

References 12 publications

Serum biomarkers in idiopathic pulmonary fibrosis

Serum biomarkers in idiopathic pulmonary fibrosis

Time‐course analysis of mouse serum proteome changes following exposure of the skin to ionizing radiation

Discovering Clinical Biomarkers of Ionizing Radiation Exposure with Serum Proteomic Analysis

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