Serum Level of Matrix Metalloproteinase-2 and -9 in Patients with Laryngeal Squamous Cell Carcinoma and Clinical Significance

Lotfi, Alireza; Mohammadi, Ghodrat; Saniee, Lale; Mousaviagdas, Mehrnoosh; Chavoshi, Hadi; Tavassoli, Atena

doi:10.7314/apjcp.2015.16.15.6749

Cited by 13 publications

(17 citation statements)

References 19 publications

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“…SOX2-OT-knockdown markedly reduced the expression of Bcl-2 protein, and increased the protein levels of Bax and cleaved caspase 3. In addition, it has been reported that increased expression of matrix metalloproteinases, particularly MMP-7 and MMP-9, is associated with occurrence and development of laryngeal carcinoma malignization (28)(29)(30). The present data suggested that the expression levels of MMP-7 and MMP-9 were markedly reduced by SOX2-OT-silencing.…”

Section: Discussionsupporting

confidence: 56%

lncRNA SOX2‑OT regulates laryngeal cancer cell proliferation, migration and invasion and induces apoptosis by suppressing miR‑654

Pan

Sun

et al. 2020

Exp Ther Med

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Laryngeal carcinoma is the most common type of malignant tumor in the head and neck. Long non-coding RNAs (lncRNAs) serve crucial roles in numerous biological processes. The present study aimed to investigate the role of lncRNA SOX2-OT in laryngeal cancer and to reveal the underlying mechanisms. Reverse transcription-quantitative PCR assays were used to measure the expression levels of SOX2-OT in the laryngeal cell lines. Furthermore, cell proliferation, apoptosis, migration and invasion were assessed by CCK-8, flow cytometry, wound healing and Transwell assays, respectively. Western blot assay was performed to detect the protein expressions. In addition, a dual-luciferase reporter assay was performed to confirm the direct interaction between SOX2-OT and microRNA (miR)-654. The data demonstrated that SOX2-OT level were significantly increased in the laryngeal cell lines. Furthermore, SOX2-OT silencing markedly promoted apoptosis and suppressed the proliferation, migration and invasion of TU-177 cells. A dual-luciferase reporter assay revealed that miR-654 was a direct target of SOX2-OT. Moreover, downregulation of miR-654 could attenuate cell apoptosis and accelerate cell proliferation, migration and invasion in TU-177 cells. In summary, the present study reported that knockdown of SOX2-OT could suppress cell proliferation, migration and invasion, and induce apoptosis in laryngeal cancer by targeting miR-654.

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Section: Discussionsupporting

confidence: 56%

lncRNA SOX2‑OT regulates laryngeal cancer cell proliferation, migration and invasion and induces apoptosis by suppressing miR‑654

Pan

Sun

et al. 2020

Exp Ther Med

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“…16 In another recent study done by Lofti A et al, where they evaluated the serum levels of MMP-2 and MMP-9 in 20 patients with OSCC and compared them to those of 20 normal subjects, both markers were found to be significantly increased in OSCC patients compared to healthy subjects. 13 The distribution of sites of OSCC among the subjects was similar to that proposed by Warnakulsuriya S., who also observed similar kind of occurrence of OSCC in their study, buccal cancer being more common among Asian populations due to betel quid/tobacco chewing habits. 5 When comparison was made based on the site of occurrence in subjects with OSCC, no significant difference in the mean levels of serum MMP-2 was observed.…”

Section: Discussionsupporting

confidence: 87%

“…MMP-2 has been detected in serum of breast cancer, prostate cancer, colorectal cancer, and laryngeal cancer patients. [10][11][12][13] Serum MMP-2 levels have been found to be increased in them as compared to the control group.…”

Section: Discussionmentioning

confidence: 90%

“…Similar results were obtained by Lofti A et al, in their study where the higher serum levels of MMP-2 were significantly correlated with increase in tumor grade (p=0.001). 13 Lastly, when mean serum MMP-2 levels in all three groups were compared, it was seen that mean serum level was higher in subjects with OSMF than normal subjects with statistically significant difference. Likewise, a statistically significant difference in mean serum MMP-2 level was seen in OSCC subjects when compared to OSMF and normal subjects.…”

Section: Discussionmentioning

confidence: 91%

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Serum levels of matrix metalloproteinase-2 in patients with oral submucous fibrosis and oral squamous cell carcinoma

Kherde

Gosavi

2020

Int J Res Med Sci

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Background: Matrix metalloproteinase-2 (MMP-2), which is supposed to enable cancer cells cross the basement membrane and metastasize by selectively cleaving type IV collagen, is anticipated to be a good diagnostic and prognostic marker in oral squamous cell carcinoma (OSCC) and oral submucous fibrosis (OSMF). Thus, the study is aimed to estimate and compare the serum MMP-2 levels in patients with OSMF and OSCC.Methods: The study was conducted on 88 subjects, divided into three groups; Group I (healthy subjects, n=28), Group II (patients with OSMF, n=30), and Group III (patients with OSCC, n=30). Serum levels of MMP-2 were estimated and compared among the groups and further with the clinical parameters within the groups.Results: The mean serum MMP-2 levels in patients with OSMF (2.87±1.04 ng/mL) and with OSCC (11.55±2.16 ng/mL) were significantly higher than the healthy subjects (0.93±0.26 ng/mL) (p <0.0001, for both). Furthermore, the mean serum MMP-2 levels in OSMF subjects had a positive association with inter-incisal opening (IIO), however, there was no association with the degree of burning sensation. Likewise, in subjects with OSCC, levels of serum MMP-2 showed positive association with histopathological grades, however, significant association with the site of occurrence and primary tumor size was not found.Conclusions: Elevated serum MMP-2 levels can be used as a screening tool in the early detection of OSMF and OSCC cases. Moreover, MMP-2 might be a good marker in evaluating the tumor grade in OSCC and the IIO in OSMF.

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“…Numerous studies have revealed that MMPs are overexpressed in several types of malignant tumours, including oral cancer [6–8]. Of the MMPs, MMP-2 and MMP-9, known as the gelatinases that degrade the main constituent of the basement membrane and type IV collagen, have been recognised as crucial in cancer invasion and metastasis.…”

Section: Introductionmentioning

confidence: 99%

Melatonin inhibits TPA-induced oral cancer cell migration by suppressing matrix metalloproteinase-9 activation through the histone acetylation

et al. 2016

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Melatonin exerts antimetastatic effects on liver and breast cancer and also inhibits matrix metalloproteinase (MMP) activity. However, the detailed impacts and underlying mechanisms of melatonin on oral cancer cell metastasis are still unclear. This study showed that melatonin attenuated the 12-O-tetradecanoylphorbol-13-acetate-induced migration of oral cancer cell lines, HSC-3 and OECM-1. Zymography, quantitative real-time PCR, and Western blotting analyses revealed that melatonin lessened MMP-9 enzyme activity as well as the expression of MMP-9 mRNA and protein. Furthermore, melatonin suppressed the phosphorylation of the ERK1/2 signalling pathway, which dampened MMP-9 gene transcription by affecting the expression of transcriptional coactivators, such as CREB-binding protein (CREBBP) and E1A binding protein p300 (EP300), and decreasing histone acetylation in HSC-3 and OECM-1 cells. Examinations on clinical samples exhibited that MMP-9, CREBBP, and EP300 were significantly increased in oral cancer tissues. Moreover, the relative level of CREBBP was positively correlated with the expression of MMP-9 and EP300. In conclusion, we demonstrated that melatonin inhibits the motility of HSC-3 and OECM-1 cells in vitro through a molecular mechanism that involves attenuation of MMP-9 expression and activity mediated by decreased histone acetylation.

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Serum Level of Matrix Metalloproteinase-2 and -9 in Patients with Laryngeal Squamous Cell Carcinoma and Clinical Significance

Cited by 13 publications

References 19 publications

lncRNA SOX2‑OT regulates laryngeal cancer cell proliferation, migration and invasion and induces apoptosis by suppressing miR‑654

lncRNA SOX2‑OT regulates laryngeal cancer cell proliferation, migration and invasion and induces apoptosis by suppressing miR‑654

Serum levels of matrix metalloproteinase-2 in patients with oral submucous fibrosis and oral squamous cell carcinoma

Melatonin inhibits TPA-induced oral cancer cell migration by suppressing matrix metalloproteinase-9 activation through the histone acetylation

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