Serum Level of egr-miR-2a-3p as a Potential Diagnostic Biomarker for Cystic Echinococcosis

Karami, Maryam Fasihi; Beiromvand, Molouk; Rafiei, Alireza; Dayer, Dian; Rahdar, Mahmoud; Bahreini, Amin; Dastyar, Ali Asghar

doi:10.1007/s11686-022-00641-2

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…One study developed a stem‐loop RT‐qPCR assay based on Echinococcus granulosus ‐derived egr‐miR‐2a‐3p, effectively distinguishing healthy subjects from cystic echinococcosis patients. ROC analysis revealed its good diagnostic value for cystic echinococcosis, with an AUC of 0.8176 17 . Another study identified two parasite‐derived miRNAs, egr‐miR‐71 and egr‐let‐7, exclusively detected in the plasma of E. granulosus ‐infected individuals.…”

Section: Discussionmentioning

confidence: 99%

“…ROC analysis revealed its good diagnostic value for cystic echinococcosis, with an AUC of 0.8176. 17 Another study identified two parasite-derived miRNAs, egr-miR-71 and egr-let-7, exclusively detected in the plasma of E. granulosus-infected individuals. Their expression levels significantly decreased at 3 and 6 months post-surgery, suggesting potential use as novel biomarkers for E. granulosus infection detection and post-surgery follow-up.…”

Section: Discussionmentioning

confidence: 99%

“…Parasite‐derived miRNAs, such as miR‐277 and miR‐3479‐3p, demonstrate promise as novel diagnostic biomarkers for detecting human Schistoma japonicum infection 16 . Additionally, research has identified egr‐miR‐2a‐3p as a potential diagnostic biomarker for cystic echinococcosis (CE) 17 . Previous studies have revealed that cestodes share the same miRNA let‐7 (let‐7‐5p), 18 which has been shown to be one of the highly expressed miRNAs in cestodes 19‐21 .…”

Section: Introductionmentioning

confidence: 99%

“…16 Additionally, research has identified egr-miR-2a-3p as a potential diagnostic biomarker for cystic echinococcosis (CE). 17 Previous studies have revealed that cestodes share the same miRNA let-7 (let-7-5p), 18 which has been shown to be one of the highly expressed miRNAs in cestodes. [19][20][21] In addition, the expression level of let-7-5p was significantly upregulated in the serum and liver of metacestodes-infected animals and humans, [22][23][24] suggesting its potential as a target molecule for diagnosing metacestodiasis.…”

Section: Introductionmentioning

confidence: 99%

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Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Wang,

Pu,

Liu

et al. 2024

The FASEB Journal

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Metacestodiasis is an infectious disease caused by the larval stage of cestode parasites. This disease poses a serious health hazard to wildlife, livestock, and humans, and it incurs substantial economic losses by impacting the safety of the livestock industry, the quality of meat production, and public health security. Unfortunately, there is currently no available molecular diagnostic method capable of distinguishing cysticercus‐ and Echinococcus‐derived microRNAs (miRNAs) from other helminthes and hosts in the plasma of metacestode‐infected animals. This study aims to develop a specific, sensitive, and cost‐efficient molecular diagnostic method for cysticercosis and echinococcosis, particularly for early detection. The study developed a rolling circular amplification (RCA)‐assisted CRISPR/Cas9 detection method based on parasite‐derived miRNA let‐7‐5p. Using a series of dilutions of the let‐7 standard, the limit of detection (LOD) of the qPCR, RCA, and RCA‐assisted CRISPR/Cas9 methods was compared. The specificity of qPCR and CRISPR/Cas9 was evaluated using four artificially synthesized let‐7 standards from different species. A total of 151 plasma samples were used to evaluate the diagnostic performance. Additionally, the study also assessed the correlation between plasma levels of let‐7‐5p, the number of Taenia pisiformis cysticerci, and the weight of Echinococcus multilocularis cysts. The results demonstrated that the RCA‐assisted CRISPR/Cas9 assay could significantly distinguish let‐7 from cestodes and other species, achieving a LOD of 10 aM; the diagnostic sensitivity and specificity for rabbit cysticercosis and mouse E. multilocularis were 100% and 97.67%, and 100% and 100%, respectively. Notably, let‐7‐5p gradually increased in the plasma of T. pisiformis‐infected rabbits from 15 days post infection (dpi), peaked at 60 dpi, and persisted until 120 dpi. In E. multilocularis‐infected mice, let‐7‐5p gradually increased from 15 dpi and persisted until 90 dpi. Furthermore, the expression of let‐7‐5p positively correlated with the number of cysticerci and cyst weight. These results indicated that the let‐7‐5p‐based RCA‐assisted CRISPR/Cas9 assay is a sensitive and specific detection method that can be used as a universal diagnostic method for metacestodiasis, particularly for early diagnosis (15 dpi).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%