Serum leptin correlates in fertile and idiopathic infertile Yemeni males: a comparative cross-sectional study

Al-Nawd, Ebraheem A.; Alshowafi, Fairouz K.; Abdullateef, Ahmed; Noman, Mohammad M. Abdulgabbar; Albadani, Rashad H.; Al-Mansoub, Majed Ahmed

doi:10.1080/19396368.2022.2113930

Cited by 1 publication

(2 citation statements)

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“…11 Some evidence indicated that leptin may have a direct negative effect on testicular steroidogenesis without inhibiting testosterone production. 12 Our previous studies reported that seminal plasma leptin levels in patients with idiopathic oligozoospermia and asthenospermia were higher than those in normal males. And seminal plasma leptin and sperm apoptosis rate were significantly increased in patients with impaired testicular spermatogenesis (varicocele and leukospermia).…”

Section: Introductionmentioning

confidence: 92%

“…And their fertility could be restored after leptin replacement therapy 11 . Some evidence indicated that leptin may have a direct negative effect on testicular steroidogenesis without inhibiting testosterone production 12 . Our previous studies reported that seminal plasma leptin levels in patients with idiopathic oligozoospermia and asthenospermia were higher than those in normal males.…”

Section: Introductionmentioning

confidence: 96%

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Leptin promotes proliferation of human undifferentiated spermatogonia by activating the PI3K/AKT/mTOR pathway

Xin,

Xiaoxuan,

Yixuan

et al. 2024

American J Rep Immunol

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BackgroundMale infertility is a common disease affecting male reproductive health. Leptin is an important hormone that regulates various physiological processes, including reproductive function. However, few experimental studies have been carried out to elucidate the mechanism of leptin's effects on male reproductive function.ObjectiveThe purpose of this study was to investigate the effects of leptin on testicular spermatogenesis and its mechanism, so as to provide potential targets for the treatment of patients with spermatogenic dysfunction.MethodsTesticular tissues were collected from eight prostate cancer patients undergoing surgical castration. GPR125‐positive spermatogonia were isolated by two consecutive magnetic activated cell sorting (MACS), followed by incubation with conditioned medium. To identify the signaling pathway(s) involved in the effects of leptin, undifferentiated spermatogonia were treated with different concentrations of leptin and antagonists of leptin‐related pathways. The proliferative effect of leptin was evaluated by cell counting using a hemocytometer. Expressions of p‐AKT, p‐ERK, p‐STAT, and p‐S6K were determined by western blotting analysis.ResultsLeptin promoted the growth of human GPR125‐positive spermatogonia in a concentration‐dependent manner. The most significant proliferative effect was observed using 100 ng/mL leptin after 6 days of culture. Leptin significantly increased the phosphorylation of STAT3, AKT, and ERK in undifferentiated spermatogonia. Phosphatidylinositol 3‐kinase (PI3K) inhibitor LY294002 inhibited the leptin‐induced activation of AKT, ERK, and downstream S6K. Treatment with the mammalian target of rapamycin (mTOR) inhibitor rapamycin also inhibited S6K phosphorylation. Moreover, both LY294002 and rapamycin were found to inhibit the leptin‐induced proliferation of undifferentiated spermatogonia. These results suggested that the leptin‐induced proliferation of GPR125‐positive spermatogonia was dependent on the PI3K/AKT/mTOR pathway. Further exploration of proliferation and apoptotic markers suggested that leptin may alleviate cell apoptosis by regulating the expression of Bax and FasL.ConclusionsA certain concentration of leptin (25∼100 ng/mL) could promote proliferation of undifferentiated spermatogonia, which was mediated by PI3K/AKT/mTOR pathway.

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Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 96%