Serum LBP Is Associated with Insulin Resistance in Women with PCOS

Abstract

IntroductionLipopolysaccharide-binding protein (LBP) is closely associated with many metabolic disorders. However, no study has been done to explore the relationship between LBP and polycystic ovary syndrome (PCOS). The objective of this study was to investigate whether the serum LBP level is elevated and associated with insulin resistance (IR) in PCOS.Participants and DesignIn this cross-sectional study, 117 PCOS patients and 121 age-matched controls were recruited. Hyperinsulinemic-euglycemic clamp was performed with an expression of M value for insulin sensitivity. Fasting serum samples were collected to detect LBP, lipids, insulin, sex hormones and high sensitive C reactive protein (hs-CRP). Pearson’s correlation and multiple linear regression was used to analyze the associations between M value and LBP level.SettingsThe study was performed in a clinical research center.ResultsCompared with controls, PCOS subjects had a significantly higher LBP concentration (33.03±14.59 vs. 24.35±10.31 μg/ml, p<0.001), and lower M value (8.21±3.06 vs. 12.31±1.72 mg/min/kg, p<0.001). Both in lean and overweight/obese individuals, serum LBP level was higher in PCOS subjects than that in controls. M value was negatively correlated with body mass index (BMI), fasting serum insulin, triglycerides, low-density lipoprotein cholesterol (LDL-c), free testosterone, high sensitive C reactive protein (hs-CRP) and LBP, whereas positively correlated with high-density lipoprotein cholesterol (HDL-c) and sex hormone binding globulin (SHBG). Serum LBP level was associated with M value after adjusting for BMI, fasting serum insulin, SHBG, as well as hs-CRP.ConclusionSerum LBP level significantly is elevated in PCOS, and is independently associated with IR in PCOS.

Sex hormone binding globulin - an important biomarker for predicting PCOS risk: A systematic review and meta-analysis

“…To our best knowledge, this is the first systematic review and meta-analysis of studies showing the potential Subtotal (I-squared = 0.0%, p = 0.848) Skrgatic L et al, 2012Joshi B et al, 2016Houjeghani S 2012Lauritsen MP et al, 2014Liu Q et al, 2008Song DK et al, 2014Meriem G et al, 2016Dikmen E et al, 2011 Subtotal (I-squared = 93.6%, p = 0.000) Pangaribuan B et al, 2012Baldani DP et al, 2015Schweighofer N et al, 2012Zhu Q et al, 2016 Case-control Study Anttila L et al, 1996Bendlov B et al, 2007Zhang Y et al, 2006ID Jamil AS et al, 2015 Cohort Study Koiou E et al, 2011Jayagopal et al, 2003Tarkun I et al, 2004El-Shal AS et al, 2013Abu HTM et al, 2016Alili A et al, 2014Chae SJ et al, 2010Prelevit GM et al, 1993 Subtotal (I-squared = 94.8%, p = 0.000) Ferk P et al, 2006Seiminska L et al, 2004Legro RS et al, 2002Tarkun S et al, 2010Atamer A et al, 2008Dadachanji R et al, 2015N. Xita et al, 2008Schüring AN et al, 2012Yasmin E et al, 2012 Cross-sectional Kim JW et al, 2012Kandasamy S et al, 2016Garcia MA et al, 2012Oh JY et al, 2009 -0.83 (-1.01, -0.64) endocrine abnormalities has been extensively studied.…”

“…A total of 39 observational cross sectional, prospective cohort, and case control studies involving 5,121 patients and 5,059 controls were included. Of all the studies, 18 were performed in Europe [Dadachanji et al 2015;Lauritsen et al 2014;Schweighofer et al 2012;Kim et al 2012;Pangaribuan et al 2012;Schüring et al 2012;Houjeghani et al 2012;Koiou et al 2011;Tarkun et al 2004;Liu et al 2008;Li et al 2006;Sieminska et al 2004;Richard 2002;Lin et al 2015;Jayagopal 2003;Elci et al 2016;Kim et al 2013;Danilowicz et al 2014], 18 in Asia [Zhu et al 2016;Jamil et al 2015;Baldani et al 2015;O'Reilly et al 2014;Martínez-García et al 2012;Alili and Idrizi 2014;El-Shal et al 2013;Skrgatic et al 2012;Dikmen 2011;Chae et al 2010;Oh et al 2009;Xita et al 2008;Bendlov et al 2007;Atamer et al 2008;Ferk et al 2007, Tarkun et al 2010Anttila et al 1996;Prelević 1993], 2 in Africa [Song et al 2014;Yasmin et al 2013], and 1 in North America [Kim et al 2014]. Studies used a variety of methods to identify patients with PCOS.…”

“…Radio Immuno Assay (RIA), Chemiluminiscence Assay (CLIA), and Electrochemiluminiscence Assay (ECLIA) were used in 4, 11, and 11 studies respectively. Five studies statistically found no significant differences in the mean SHBG levels [Zhu et al 2016;Schweighofer et al 2012;Dikmen 2011;Atamer et al 2008;Ferk et al 2007]. According to the quality scoring assessment checklist, 8 studies having a score of more than 8 were considered high quality studies [Zhu et al 2016;Jamil et al 2015;Baldani et al 2015, Alili andIdrizi 2014;Pangaribuan et al 2012;Dikmen 2011;Koiou et al 2011;Tarkun et al 2010].…”

Lispro administered by the QS-M Needle-Free Jet Injector generates an earlier insulin exposure

“…In phase I, nine subjects were administered with lispro (Humalog, Eli Lilly and Company, US) 0.2 IU/kg by the QS-M needle-free jet injector (Beijing QS Medical Technology Co., Ltd., China) and comparable volume of placebo solution (placebo solution consisted of normal saline, which was injected into an empty insulin vial under aseptic conditions) by an insulin pen (HumaPen ErgoII, Eli Lilly and Company, US) in the abdomen simultaneously; the remaining nine subjects were administered with lispro 0.2 IU/ kg by a conventional pen and a comparable volume of placebo solution by a needle-free jet injector in the abdomen simultaneously. Seven-hour euglycemic glucose clamp tests were then performed by an experienced nurse [12][13][14]. Two catheters were inserted intravenously.…”

A Review on the Link between Psoriasis Vulgaris and Polycystic Ovary Syndrome

“…Two of the following criteria are sufficient for diagnosis: oligo-or anovulation, biochemical or clinical hyperandrogenism, and polycystic ovaries on ultrasound examination [11]. Similar to psoriasis, the components of metabolic syndrome [12][13][14], obesity [13], diabetes [15], insulin resistance [16][17][18][19][20], dyslipidemia [21], hypertension [15,[22][23], and coronary artery disease [15] are reported to be closely related to PCOS. Based on these similarities, with the support of the literature, female patients with psoriasis in the reproductive period are thought to have a higher risk of PCOS [24][25][26].…”