Serum Isomerase in Leukemia

Abstract

Bodansky (1954a) recently described a method for the determination of serum isomnerase and examined the extent to which the serum enzyme activity can be correlated with clinical and biochemical evidence of tumour growth. He found good correlation in breast and prostatic cancer (Bodansky, 1954b(Bodansky, , 1955.In view of the considerable isomerase activity shown by erythrocytes and leukocytes, we have determined the serum isomerase concentrationi in diseases involving the cellular elements of the blood. MIIATERIALS AND METHODSThe serum phosphohexose isomerase activity was determined according to the mnethod of Bodansky (1954a). This determination was carried out on fresh serum obtained from normal controls, patients with variousnon-malignantdiseaseswith normal leukocyte counts, patients with chronic myelocytic and chronic lymphocytic leukaemia, and patients with leukocytosis due to infection. Many of the leukemia group were under treatment at the time of isomerase estimation. Those patients with chronic myelocytic leukemia were receiving radiation or dimethylsulphonoxylbutane (Myleran®), and those with chronic lymphocytic leukemia, radiation or p-(Di-2-chloroethylamino)-phenylbutyric acid (CB1348). Fig. 2 shows the relationship between the serum isomerase and the number of cells of the myelocytic series in chronic myelocytic leukemia before and after treatment, chronic lymphocytic leukemia and infection. In the chronic myelocytic

Serum phosphohexose isomerase levels in patients with head and neck cancer

“…Our results in the present study demonstrate that serum PHI levels were increased by more than 100 per cent in patients with head and neck cancer (Table I). Serum PHI levels have been shown to be raised by nearly l-to-18 fold in various types of cancer (Israels and Delory, 1956;Schwartz et al, 1962a, b;Griffith and Beck, 1963;Damle et al, 1971). The rise, however, was less in cancer of the head and neck region, as compared to that of the breast, prostate or liver.…”

A routine method for the determination of phosphoglucose isomerase activity in body fluid

“…The serum activity is greatly increased in acute hepatitis (Bruns and Jacob, 1954;Bruns, 1957;Bing et al, 1957;Bodansky, Krugman, Ward, Schwartz, Giles, and Jacobs, 1959). Israels and Delory (1956), Israels, Delory, Hnatiuk, and Friesen (1958) and Blanchaer, Green, MacLean, and Hollenberg (1958) found raised activity in myelocytic leukaemia but not in the leucocytosis of infection or in lymphocytic leukaemia. Bodansky, Gershten, and Wilson (1954) and Myers and Bodansky (1957) reported the assay to be useful in following progress during palliative treatment for metastatic carcinoma of the breast and also of the prostate (Bodansky, Calitri, and Wilson, 1955).…”