Serum Insulin-like Activity in Hypophysectomized and Depancreatized (Houssay) Dogs

Schoeffling, K; Ditschuneit, H.; Petzoldt, R; Beyer, J.; Pfeiffer, E. F.; Sirek, Anna; Geerling, H.; Sirek, O. V.

doi:10.2337/diab.14.10.658

Cited by 26 publications

(7 citation statements)

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“…In the rat, for instance, the administration of streptozotocin which leads to a diabetic state and which is less severe than that following pancrea¬ tectomy, does not appear to be followed by a very marked fall in the NSILA-S levels . Reports from other laboratories dealing with ILA and NSILA after pancreatectomy are controversial (Steinke et al 1962;Schoeffling et al 1965;Edgahl 8c Goldberg 1962). This is best explained by the fact that NSILA or ILA was measured in whole serum without extraction so that the effect of a multitude of stimulatory and inhibitory factors was observed rather than that of NSILA-S proper (Oelz et al 1972).…”

Section: Discussionmentioning

confidence: 96%

Decrease of Non-Suppressible Insulin-Like Activity After Pancreatectomy and Normalization by Insulin Therapy

Eigenmann

Becker

Kammermann

et al. 1977

Acta Endocrinologica

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Non-suppressible insulin-like activity (NSILA-S) was determined in 5 dogs before and after pancreatectomy and again during insulin therapy. All NSILA-S determinations were carried out on serum samples which were passed over Sephadex G-50 columns equilibrated with 1 m acetic acid. The levels of NSILA-S decreased drastically shortly after pancreatectomy and rose slowly after institution of insulin therapy, to normal levels. During the period of severe diabetes after pancreatectomy the concentration of growth hormone was elevated. These findings indicate that 1) the pancreas cannot be the site of synthesis and release of NSILA-S, 2) NSILA-S levels do not always parallel growth hormone levels and 3) the synthesis and secretion of NSILA-S among other factors is under the control of insulin.Insulin-like effects of non-suppressible insulin-like activity (NSILA-S) have been extensively studied in vitro and in vivo (for review see Oelz et al. (1972)).

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Section: Discussionmentioning

confidence: 96%

Decrease of Non-Suppressible Insulin-Like Activity After Pancreatectomy and Normalization by Insulin Therapy

Eigenmann

Becker

Kammermann

et al. 1977

Acta Endocrinologica

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“…This concept was substantiated by the development of the more specific insulin immunoassay. Normal fasting serum levels of insulin derived from immunoassay data have approximated 15-20 uU/ml (14), whereas fasting levels of "insulin" measured in vitro bioassay techniques not altered by various physiological and pathological states, its biological significance has been questioned (6)(7)(8). Nevertheless, variations in serum ILA have been observed under certain conditions, and a possible physiological role has been implied (9,10).…”

Section: Introductionmentioning

confidence: 99%

The nature of human serum insulin-like activity (ILA): characterization of ILA in serum and serum fractions obtained by acid-ethanol extraction and adsorption chromatography

Poffenbarger¹,

Ensinck²,

Hepp³

et al. 1968

J. Clin. Invest.

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AB STRACT Studies were undertaken in an attempt to clarify the apparent heterogeneous nature of human serum insulin-like activity. Methods of preparative zone electrophoresis on Pevikon, acid-ethanol extraction of trichloroacetic acid serum protein precipitates, adsorption chromatography on DEAE-cellulose and Dowex 50, gel filtration chromatography, and insulin antiserum immunoreactivity were used. The results establish the presence of a substance in serum with in vitro biological properties similar to insuln but with different physicochemical properties. The major portion of serum ILA measured by bioassay techniques can be attributed to the effects of this substance. Whereas the in vitro biological effects of this substance on muscle and adipose cells were similar to those of crystalline insulin, the substance is distinguished from insulin by: (1) the failure of insulin antiserum to inhibit its in vitro biological effect; (2) a slower electrophoretic mobility (in the gamma-beta globulin zone); and (3) a larger molecular weight, between 40,000 and 50,-000 in these studies. It is similar to insulin since both are soluble in acid-ethanol. The results furThis work was presented in part as an abstract at the 26th Annual Meeting of The American Diabetes Association.

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“…72 However, a similar activity has recently been observed, manifesting constant (50 per cent) levels over a long period of time in the hypophysectomized pancreatectomized dog. 73 Crystalline insulin, regardless of origin, exhibits an identical effect on adipose tissues of a given species. However, when adipose tissues from different species are used, there are wide variations in its degree of activity.…”

Section: Assays: Comparison and Specificitymentioning

confidence: 97%

Insulin-like Activity

Batchelor

1967

Diabetes

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This presentation is a review of the ILA concept and considers (1) major insulin assays; description, comparison, and variation of results; (2) location of circulating ILA in the plasma proteins by various technics; (3) types of ILA: "free-bound," "typical-atypical," "suppressiblenonsuppressible," "NSILA," and other forms; (4) inhibitory or antagonistic factors: synalbumin-antagonistic albumin and nonesterified fatty acids; and (5) the ILA concept with respect to vascular membranes and the glucose transport theory. Also included are data comparing insulin levels obtained by the major assays, types of ILA-insulin levels and properties of the various forms of insulin. DIABETES 76: 418-34, June, 1967. During the past ten years much thought and investigative effort have been spent on the concept of insulinlike activity (ILA). Answers to questions pertaining to its origin, chemical composition, and possible physiologic role may well prove important in understanding the diabetic state. The purpose of this discussion will be to consider these questions. Since the concept of ILA is based on the significant differences in circulating insulin levels that result from the three major forms of insulin assay-(i) rat epididymal fat pad, (2) rat diaphragm, and (3) the radioimmunologic assay-it begins with a description of these technics. INSULIN ASSAYS IN VITROThe first type of biologic insulin assay in vitro, utilizing the isolated rat hemidiaphragm, was developed in the early 1950's. It is based on studies showing that small amounts of insulin accelerate both glucose utilization and (perhaps more specifically) glycogen synthesis by the rat diaphragm, 4 " 0 and that a quantitative relationship exists between the concentration of insulin and these effects. 7 ' 8

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Serum Insulin-like Activity in Hypophysectomized and Depancreatized (Houssay) Dogs

Cited by 26 publications

References 0 publications

Decrease of Non-Suppressible Insulin-Like Activity After Pancreatectomy and Normalization by Insulin Therapy

Decrease of Non-Suppressible Insulin-Like Activity After Pancreatectomy and Normalization by Insulin Therapy

The nature of human serum insulin-like activity (ILA): characterization of ILA in serum and serum fractions obtained by acid-ethanol extraction and adsorption chromatography

Insulin-like Activity

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