Serum-induced macrophage activation is related to the severity of septic shock

Peck, George C.; Andrades, Michael Éverton; Lorenzi, Rodrigo; Costa, M. Da; Petronilho, Fabrícia; Moreira, Jcf; Dal‐Pizzol, Felipe

doi:10.1007/s00011-009-8088-z

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…The monocyte/macrophage content was compared with that of age-matched organs from deaths that had occurred in the intensive care unit. Patients dying with sepsis, with inflammation, and after ventilation often show a tissue pattern of macrophage activation syndrome, making this a ''high normal'' control [3][4][5][6]. Our patient's bone marrow showed numerous CD68 PGM-1-positive/ CD163-negative osteoclasts and a few osteoblasts but only rare hematopoietic elements and virtually no stroma ( Fig.…”

Section: Case Reportmentioning

confidence: 91%

A Novel TCIRG1 Gene Mutation Leads to Severe Osteopetrosis with Altered Content of Monocytes/Macrophages in Several Organs

et al. 2012

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Osteopetrosis (OP) is a clinically and genetically heterogeneous disease. Defects in the TCIRG1 gene are most frequently implicated in the osteoclast-rich form of OP. Little is known about the content and/or function of monocytes and macrophages of various organs rich in those cells in patients with OP. We report a patient with a novel TCIRG1 gene mutation that led to an osteoclast-rich OP. A bone marrow transplant failed to engraft, and the patient developed pulmonary hypertension. At autopsy he was found to have abnormal remodeling of the pulmonary vasculature and alveolar proteinosis. Alveolar macrophages were decreased. Pulmonary findings in this patient could be at least partially explained by abnormal surfactant metabolism due to depleted or defective alveolar macrophages.

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Section: Case Reportmentioning

confidence: 91%

A Novel TCIRG1 Gene Mutation Leads to Severe Osteopetrosis with Altered Content of Monocytes/Macrophages in Several Organs

et al. 2012

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“…It is known that the serum of septic patients is able to induce the activation of macrophages and the production of IL-10; also, the phagocytic indices of these macrophages were correlated with the severity and mortality of the disease (Peck et al 2009). Murine models of lethal sepsis have higher protein carbonyl levels and an imbalance in SOD/CAT (superoxide dismutase/ catalase) when compared with the nonlethal group, indicating that oxidative stress is exacerbated in the lethal model (Andrades et al 2005).…”

Section: Introductionmentioning

confidence: 99%

The oxidation of HSP70 is associated with functional impairment and lack of stimulatory capacity

Grunwald

Pires

Zanotto-Filho

et al. 2014

Cell Stress and Chaperones

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Expression of intracellular HSP70 is associated with cytoprotective effects against a wide range of stressful stimuli, such as inflammation, oxidative stress, hypoxia, endotoxins, infections, and fever. This cytoprotective effect is mainly attributed to their ability to stabilize protein structures through chaperone-like reversible interactions. HSP70 was recently detected in the extracellular medium, and its presence in serum is commonly associated with pathological situations, where it exerts modulatory effects on cells of the immune system. Previously, we have described the relationship between serum HSP70 levels, oxidant status, and clinical outcome of septic patients; the group of patients with higher prooxidant status and higher serum HSP70 had also higher mortality. To investigate the possible association between oxidized HSP70 and cytoprotection or cell death, we incubated RAW 264.7 macrophages with oxidized HSP70 and evaluated nitrite production, cell proliferation, cell viability, TNF-α release, and phagocytic activity. We also evaluated structural modifications caused by oxidation in purified HSP70. Oxidation of HSP70 altered its protein structure; besides, the modulatory effect of oxidized HSP70 on RAW264.7 cells was different from that of native HSP70. Macrophages treated with oxidized HSP70 presented lower proliferation and viability, lower phagocytic activity, and lower TNF-α release. These results indicate that oxidation of extracellular HSP70 modified its signaling properties, causing alterations on its modulatory effects on macrophage function and viability.

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Nitric Oxide in Sepsis and Hemorrhagic Shock: Beneficial or Detrimental?

Loughran

Zhao

et al. 2017

Nitric Oxide

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Serum-induced macrophage activation is related to the severity of septic shock

Abstract: Markers of antiinflammation are predominant in more severe types of sepsis suggesting that antiinflammation is related to mortality.

Cited by 4 publications

References 29 publications

A Novel TCIRG1 Gene Mutation Leads to Severe Osteopetrosis with Altered Content of Monocytes/Macrophages in Several Organs

A Novel TCIRG1 Gene Mutation Leads to Severe Osteopetrosis with Altered Content of Monocytes/Macrophages in Several Organs

The oxidation of HSP70 is associated with functional impairment and lack of stimulatory capacity

Nitric Oxide in Sepsis and Hemorrhagic Shock: Beneficial or Detrimental?

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