Serum IgG Responses to gp15 and gp40 Protein-Derived Synthetic Peptides From Cryptosporidium parvum

Abstract

Cryptosporidium spp. are responsible for moderate to severe diarrhea, mainly in children and immunocompromised patients. Using ELISA, the recognition of synthetic peptides generated from the sequences of the Cryptosporidium parvum gp40 and gp15 proteins by serum IgM and IgG antibodies from patients infected (cases) with Cryptosporidium hominis, C. parvum, and Cryptosporidium canis, and uninfected individuals (controls) was evaluated. A statistically significant difference (p = 0.0025) was found in terms of the recognition of peptides A133 and A32 between cases and controls. Additional studies are necessary to understand the potential of these peptides as vaccine candidates.

Specific Cryptosporidium antigens associate with reinfection immunity and protection from cryptosporidiosis

“…Likewise, Chro.30111 antibody responses showed evidence of protection during followup and at the end of the first year of followup postsampling, but not at the end of 2 years of followup. While plasma IgG anti-Cp23 and Cp17 had not been previously observed to be protective in our cohort (8,10), reanalysis of the IgG data in our study suggests that our use of the mixed models to determine the boundary of the positive response in the protein array data improved the specificity of the immunoassay results and accounted for the apparent difference in the study conclusions (Supplemental Figure 4) (7,44).…”

Control of the Waterborne Cryptosporidiosis: Evaluation of the Protective Role of Cryptosporidium parvum Oocysts Antigen in Infected Immunocompetent and Immunosuppressed Mice

“…Cryptosporidium sp. causes moderate to severe diarrhea, which is more common in young and immunocompromised people (Urrea-Quezada et al, 2022). Infections can develop from low doses of Cryptosporidium oocysts; so, it has major public health implications and can persist in the environment for long periods of time.…”