Serum IgG galactosylation in psoriatic arthritis patients undergoing a biological treatment. A preliminary report

Jakubiak-Augustyn, Anna; Sokolik, Renata; Duk, Maria; Szechiński, Jacek; Wiland, Piotr; Lisowska, Elwira; Sobczyński, Maciej; Krotkiewski, Hubert

doi:10.5114/reum.2014.47230

Cited by 1 publication

(2 citation statements)

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“…It should also be mentioned that in lectin-ELISA method, glycan-lectin reaction reflect the reactions that occur in living organisms, including the availability of sugar residues for lectins, which additionally allows to deepen the knowledge about mechanisms of these interactions. The profile and degree of IgG sialylation and galactosylation/agalactosylation was determined using modified solid phase enzyme-linked immunosorbent assay, lectin-ELISA described by us earlier [31][32][33][34] . The method was based on the reactivity of IgG glycan moieties with specific biotinylated lectins: Maackia amurensis agglutinin (MAA, recognizing sialic acid α2,3-linked) and Sambucus nigra agglutinin (SNA, which recognize sialic acid α2,6-linked), Ricinus communis agglutinin I (RCA-I) which detect the terminal galactose; Griffonia simplicifolia lectin II (GSL-II) detecting the terminal GlcNAc (Vector Laboratories Inc., Burlingame, CA, USA).…”

Section: Discussionmentioning

confidence: 99%

“…Another important aspect was the comparison of native serum IgG glycosylation with glycosylation of IgG isolated from serum, and evaluation the potential diagnostic suitability of glycan analysis when glycoprotein is not isolated earlier from biological fluid. The degree of IgG sialylation and galactosylation/agalactosylation was analysed using a modified solid phase enzyme-linked immunosorbent assay, lectin-ELISA [31][32][33][34] .…”

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confidence: 99%

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Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis

Sołkiewicz

Krotkiewski

Jędryka

et al. 2021

Sci Rep

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Endometriosis is an inflammatory disease which diagnostics is difficult and often invasive, therefore non-invasive diagnostics methods and parameters are needed for endometriosis detection. The aim of our study was to analyse the glycosylation of native serum IgG and IgG isolated from sera of women classified as: with endometriosis, without endometriosis but with some benign ginecological disease, and control group of healthy women, in context of its utility for differentiation of advanced endometriosis from the group of healthy women. IgG sialylation and galactosylation/agalactosylation degree was determined using specific lectins: MAA and SNA detecting sialic acid α2,3- and α2,6-linked, respectively, RCA-I and GSL-II specific to terminal Gal and terminal GlcNAc, respectively. The results of ROC and cluster analysis showed that the serum IgG MAA-reactivity, sialylation and agalactosylation factor may be used as supplementary parameters for endometriosis diagnostics and could be taken into account as a useful clinical tool to elucidate women with high risk of endometriosis development. Additionally, we have shown that the analysis of native serum IgG glycosylation, without the prior time-consuming and expensive isolation of the protein, is sufficient to differentiation endometriosis from a group of healthy women.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%