Serum Ige Studies in Atopic Dermatitis

Clendenning, William E.; Clack, W E; Ogawa, Makio; Ishizaka, Kimishige

doi:10.1111/1523-1747.ep12676481

Cited by 70 publications

(32 citation statements)

References 11 publications

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“…Clinical observations have suggested a mild correlation between serum IgE levels and the extent and/or severity of disease, especially with cutaneous lesions [4,5]. The antigen-specific IgE bound to Fcε receptors expressed on mast cells induces the release of inflammatory mediators, such as histamine, leukotrienes, prostaglandins and cytokines, in response to the binding of multivalent antigens, which subsequently cross-links the Fcε receptors.…”

Section: Introductionmentioning

confidence: 99%

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Suzuki¹,

Oomizu²,

Yanase³

et al. 2009

Int Arch Allergy Immunol

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Background: Oral administration of pulverized Konjac glucomannan (KGM) reduces increased plasma IgE and the amount of Ε-germline transcript (ΕGT) in the spleen, as well as preventing the development of dermatitis in mice. To elucidate the mechanism of action of pulverized KGM, we solubilized KGM and studied its effect on IgE in vitro and in vivo. Methods: Solubilized KGM was prepared by acid hydrolysis, and we analyzed the effective molecular size for the suppression of IgE production and ΕGT in vitro and the level of plasma IgE induced by immunization with ovalbumin in BALB/c mice. Results: The production of IgE and ΕGT in splenic cells, but not purified B cells, was inhibited by hydrolyzed KGM (KGM hydrolyzed with 0.25 N HCl; H-KGM) at the optimal size of between 10 and 500 kDa. However, no effect was observed when H-KGM was substituted with unhydrolyzed KGM in vitro. IgE production from purified B cells cocultured with purified monocytes, but not with purified T cells, was inhibited by H-KGM. The release of IFNγ in cultures of monocytes but in purified B cells with or without T cells was enhanced in the presence of H-KGM. Injection of mice with H-KGM also suppressed the production of plasma IgE and IgG1 but not IgG2a in vivo. Conclusion: KGM at an optimal size prevents germline class-switching and IgE production both in vitro and in vivo. H-KGM may be useful as a tool to study the mechanism of action of KGM and as a dietary supplement to prevent atopic diseases.

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Section: Introductionmentioning

confidence: 99%

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Suzuki¹,

Oomizu²,

Yanase³

et al. 2009

Int Arch Allergy Immunol

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“…However, assessment methods are far from being standardized, and therapeutic studies are often difficult to interpret. Efforts have been done recently to classify patients in groups of severity [2][3][4] and to estab lish simple and reliable assessment methods [5][6][7][8][9][10][11]. Var iants of the scoring system proposed in psoriasis (PASI score [12]) have been published [13.…”

Section: Introductionmentioning

confidence: 99%

Severity Scoring of Atopic Dermatitis: The SCORAD Index

Schallreuter¹,

Levenig²,

Berger³

et al. 1993

Dermatology

1,865

270

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Background. Assessment methods for atopic dermatitis (AD) are not standardized, and therapeutic studies are difficult to interpret. Aims. To obtain a consensus on assessment methods in AD and to use a statistical method to develop a composite severity index.Methods. Consensus definitions were given for items used in the scoring system (extent, intensity, subjective) and illustrated for intensity items. Slides were reviewed to address within and between-observer variability by a group of 10 trained clinicians, and data were statistically evaluated with a two way analysis of variance. Two variants of an assessment system were compared in 88 patients at 5 different institutions. Data were analyzed using principal-component analysis. Results. For 5 intensity items studied (erythema, edema/papulation, oozing/crusts, excoriations, lichenification), within- and between-observer variability was good overall, except for edema/papulation which was difficult to assess with slides. In the series of 88 patients, principal-component analysis allowed to extract two unrelated components: the first one accounting for 33% of total variance was interpreted as a ‘severity’ component; the second one, accounting for 18% of variance, was interpreted as a ‘profile’ component distinguishing patients with mostly erythema and subjective symptoms and those with mostly lichenification and dryness and lower subjective symptoms. Of the two evaluation systems used, the one using the rule of nine to assess extent was found more workable than the one using a distribution × intensity product. A scoring index (SCORAD) combining extent, severity and subjective symptoms was mathematically derived from the first system and showed a normal distribution of the population studied. Conclusion. The final choice for the evaluation system was mostly made based on simplicity and easy routine use in outpatient clinics. Based on mathematical appreciation of weights of the items used in the assessment of AD, extent and subjective symptoms account for around 20% each of the total score, intensity items representing 60%. The so-designed composite index SCORAD needs to be further tested in clinical trials.

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“…While a clinical sensitivity can only be confirmed by carefully controlled challenge and avoidance studies [4,5], it remains important to accurately quantitate the foodspecific IgE antibody response. As skin reactivity may not accurately reflect circulating food-specific IgE antibody [6], and radioallergosorbent test (RAST) can be adversely affected by the very high total IgE le vels present in these patients [7,8], it is likely that no single approach to assessment is adequate.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Skin Reactivity and Specific Antibody to Egg and Milk Proteins in Patients with Atopic Dermatitis: Comparison with Responses to the House Dust Mite Antigen

Rowntree¹,

Mitchell²,

Platts-Mills³

1986

Int Arch Allergy Immunol

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Sixty patients with atopic dermatitis attending an allergy clinic were assessed for evidence of skin sensitivity and serum antibodies to egg and milk proteins. Prick skin test responses to egg were found in 23 patients and in 74% of these positive egg radioallergosorbent test (RAST) was demonstrable. Positive prick test for milk were present in 10 patients, but only 30% gave a positive milk RAST. Quantitative intradermal skin testing, RAST, and a double antibody antigen binding radioimmunoassay confirmed the presence of IgE antibody to egg proteins but indicated that the levels were very low when compared to those seen to the house dust mite antigen in sensitive patients. In contrast, IgG antibody to purified egg and milk proteins was present in large amount in most patients, the levels being significantly higher than in non-allergic controls.

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Serum Ige Studies in Atopic Dermatitis

Cited by 70 publications

References 11 publications

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Severity Scoring of Atopic Dermatitis: The SCORAD Index

Assessment of Skin Reactivity and Specific Antibody to Egg and Milk Proteins in Patients with Atopic Dermatitis: Comparison with Responses to the House Dust Mite Antigen

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