“…Using ELISA, the qualitative and quantitative differences between class-specific antibodies (IgA, IgE, IgG, and IgM) can be determined. In general, IgG and IgM antibodies, but not IgA antibodies, develop against vaccine antigens following primary immunization (146,332,579,757,821). Interestingly, persons who have been primed by infection respond to immunization with not just an IgG response but also an IgA response (446).…”
Bordetella respiratory infections are common in people (B. pertussis) and in animals (B. bronchiseptica). During the last two decades, much has been learned about the virulence determinants, pathogenesis, and immunity of Bordetella. Clinically, the full spectrum of disease due to B. pertussis infection is now understood, and infections in adolescents and adults are recognized as the reservoir for cyclic outbreaks of disease. DTaP vaccines, which are less reactogenic than DTP vaccines, are now in general use in many developed countries, and it is expected that the expansion of their use to adolescents and adults will have a significant impact on reducing pertussis and perhaps decrease the circulation of B. pertussis. Future studies should seek to determine the cause of the unique cough which is associated with Bordetella respiratory infections. It is also hoped that data gathered from molecular Bordetella research will lead to a new generation of DTaP vaccines which provide greater efficacy than is provided by today's vaccines
“…Using ELISA, the qualitative and quantitative differences between class-specific antibodies (IgA, IgE, IgG, and IgM) can be determined. In general, IgG and IgM antibodies, but not IgA antibodies, develop against vaccine antigens following primary immunization (146,332,579,757,821). Interestingly, persons who have been primed by infection respond to immunization with not just an IgG response but also an IgA response (446).…”
Bordetella respiratory infections are common in people (B. pertussis) and in animals (B. bronchiseptica). During the last two decades, much has been learned about the virulence determinants, pathogenesis, and immunity of Bordetella. Clinically, the full spectrum of disease due to B. pertussis infection is now understood, and infections in adolescents and adults are recognized as the reservoir for cyclic outbreaks of disease. DTaP vaccines, which are less reactogenic than DTP vaccines, are now in general use in many developed countries, and it is expected that the expansion of their use to adolescents and adults will have a significant impact on reducing pertussis and perhaps decrease the circulation of B. pertussis. Future studies should seek to determine the cause of the unique cough which is associated with Bordetella respiratory infections. It is also hoped that data gathered from molecular Bordetella research will lead to a new generation of DTaP vaccines which provide greater efficacy than is provided by today's vaccines
“…Various ELISAs for detection of pertussis antibodies use different antigen preparations, including purified FHA and PT (23), partially purified FHA (57,60), whole cells (54,114), and whole-cell sonic extracts (84,103,104,129,159). Onorato and Wassilak suggest that, while use of whole-cell or bacterial sonic extracts may increase the sensitivity of the ELISA, it may also increase the risk of cross-reactivity with other microorganisms (121).…”
Bordetella pertussis, the causative agent of whooping cough, produces an acute and chronic respiratory infection in infants and young children. B. pertussis is still a major health problem of young children throughout the world even though effective immunization against whooping cough is available. While predominantly a childhood disease, it has been reported also to be a cause of persistent cough in adults. This review discusses the numerous bacterial virulence factors that may play roles in the pathogenesis of pertussis and in immunity to infection. The present problems with pertussis diagnosis, recent advances, and future prospects for new and improved rapid diagnostics tests also are explored.
“…Table IV shows that negative antibody results in culture-negative children with pertussis were particularly common in the 6-month-3 year age-range, possibly owing to an immaturity of IgA antibody response at that age. Nagel and Poot-Scholtens (1983), using an ELISA for serum IgA antibody, found that the time after onset for most babies < 1 year old to produce antibody was 2 6 weeks, compared with around 3 weeks for children > 1 year old. Another explanation for some negative results could be that the antibody test used is not as sensitive as it could be.…”
Summary. Pernasal aspirate (PNA) was obtained from 543 children during a 6-month period when whooping cough was prevalent. Three tests for diagnosing pertussis were performed on the PNA : (a) examination of direct smears by immunofluorescence (IF) for Bordetella pertussis; (b) culture ; and (c) estimation of B. pertussis-specific immunoglobulin-A antibody (P-IgA) by an enzyme-linked immunosorbent assay (ELISA). On clinical review, 395 children were assessed to have had pertussis (P children) and 148 children not to have had pertussis (non-P children). The non-P children comprised 66 admitted to hospital for acute respiratory infections and 82 outpatients suspected of having pertussis. Analysis of the results of the tests on the PNAs of the non-P children helped to assess the P-IgA test. The analysis showed that artificial immunisation against pertussis did not affect the antibody results, but that non-specific positive results occur requiring the labelling of many P-IgA results as "doubtful". Among the 395 P children, 36% yielded positive cultures and more than half of these also had positive IF tests. The ELISA for P-IgA was positive in 24% of all the P children, equivalent to nearly 40% of the culture-negative P children. For the 148 non-P children, IF and culture-negative by definition, the P-IgA test was positive in 9%. The antibody test result was doubtful in 28% of the P children and in 40% of the non-P children. Estimation of P-IgA antibodies in PNA is a useful and economic complement to culture and IF in the diagnosis of pertussis. The occurrence of non-specific positive results makes evaluation of the clinical features of the child essential to the interpretation of the test.
IntroductionIn affluent countries, whooping cough is a rare cause of death. For this reason the frequency with which the infection causes prolonged family distress is liable to be discounted. Johnston et al. (1985) recently wrote : "Parents suffered especially from fears for the life and health of their child and from loss of sleep". In the absence of an effective way of shortening the course of the illness (Broomhall and Herxheimer, 1984), prevention continues to be the only practical option.
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