Serum <i>N</i>‐glycan profiling as a diagnostic biomarker for the identification and assessment of psoriasis

Zou, Chengyun; Huang, Chenjun; Li, Yan; Li, Xin; Xing, Meng; Li, Bin; Gao, Chunfang; Wang, Haiying

doi:10.1002/jcla.23711

Cited by 8 publications

(7 citation statements)

References 46 publications

(65 reference statements)

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“… 12 Nowadays, the disease‐related glycosylation alterations of serum and IgG have shown great potential in the biomarker field. 12 , 13 , 14 , 15 , 16…”

Section: Introductionmentioning

confidence: 99%

N‐glycan profiling alterations of serum and immunoglobulin G in immune thrombocytopenia

Wang

Huang

et al. 2021

Clinical Laboratory Analysis

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Background The glycosylation alterations of serum and IgG are involved in a variety of autoimmune and inflammatory diseases and have shown great potential in biomarker field. The diagnosis of immune thrombocytopenia (ITP) is exclusive. Our study aimed to discover the potential glyco‐biomarkers for auxiliary diagnosis of ITP. Methods The serum samples were obtained from 61 ITP patients and 35 healthy controls, and IgG samples were purified from 34 out of 61 ITP patients and 35 healthy controls. DNA sequencer‐assisted fluorophore‐assisted carbohydrate electrophoresis (DSA‐FACE) was used to analyze serum and IgG N‐glycan profiling. Results 6 of 12 serum N‐glycan peaks, 6 of 7 IgG N‐glycan peaks, serum fucosylation, and IgG galactosylation were significantly different between ITP patients and healthy controls (p < 0.05). IgG peak 7 showed good diagnostic efficacy for discriminating ITP patients from healthy individuals (AUC 0.967). ITP patients with severe thrombocytopenia had a significantly lower serum fucosylation than ITP patients with mild and moderate thrombocytopenia (p < 0.05). Serum fucosylation and serum peak 5 were correlated with platelet counts in ITP patients with severe thrombocytopenia, and the absolute values of correlation coefficient were both over 0.5. Conclusions The specific N‐glycan patterns of serum and IgG were observed in ITP patients. IgG peak 7 was a potential biomarker for auxiliary diagnosis of ITP.

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“… 12 Nowadays, the disease‐related glycosylation alterations of serum and IgG have shown great potential in the biomarker field. 12 , 13 , 14 , 15 , 16…”

Section: Introductionmentioning

confidence: 99%

N‐glycan profiling alterations of serum and immunoglobulin G in immune thrombocytopenia

Wang

Huang

et al. 2021

Clinical Laboratory Analysis

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“…Another study of serum analysis identified 12 different N-glycan, where 4 N-glycan were significantly increased in psoriasis patients compared to healthy subjects, and another 4 were decreased. The level of one N-glycan was increased gradually with the disease severity and could be a promising biomarker in early diagnosis of psoriasis and be exerted in progression monitoring [108]. The above findings suggest that glycosylation can be involved in the pathogenesis of psoriasis, but enlarged patient groups are needed for clinical validation.…”

Section: Post-translational Modifications (Ptms) Of Proteinsmentioning

confidence: 87%

Proteomic and Metabolomic Changes in Psoriasis Preclinical and Clinical Aspects

Radulska,

Pelikant-Małecka,

Jendernalik

et al. 2023

IJMS

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Skin diseases such as psoriasis (Ps) and psoriatic arthritis (PsA) are immune-mediated inflammatory diseases. Overlap of autoinflammatory and autoimmune conditions hinders diagnoses and identifying personalized patient treatments due to different psoriasis subtypes and the lack of verified biomarkers. Recently, proteomics and metabolomics have been intensively investigated in a broad range of skin diseases with the main purpose of identifying proteins and small molecules involved in the pathogenesis and development of the disease. This review discusses proteomics and metabolomics strategies and their utility in research and clinical practice in psoriasis and psoriasis arthritis. We summarize the studies, from in vivo models conducted on animals through academic research to clinical trials, and highlight their contribution to the discovery of biomarkers and targets for biological drugs.

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“…As an N-glycan spectrum detection technique, DSA-FACE has the advantages of high sensitivity, high throughput, high speed, simple operation, and high efficiency. It has been widely used in the detection of N-glycan in cancer and other diseases (19,20). In this study, we used this technique to analyze the serum N-glycan of patients with liver diseases and healthy subjects.…”

Section: Discussionmentioning

confidence: 99%

Serum N-glycan profiling as a diagnostic biomarker for the identification of hepatitis B virus-associated hepatocellular carcinoma

Guo¹,

Wan²,

Hu³

et al. 2022

J Gastrointest Oncol

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Background: Changes in N-glycosylation of proteins are thought to play a key role in cancer. This study aims to investigate the changes in the serum N-glycan profiles of patients with hepatitis B virus (HBV)-related liver disease, and to evaluate the role of N-glycan markers in the noninvasive diagnosis of hepatocellular carcinoma (HCC).Methods: Serum samples were available for 21 patients with HCC, 20 patients with liver cirrhosis (LC), 20 patients with chronic hepatitis B (CHB), and 20 healthy subjects. Serum N-glycans were released and analyzed using DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE). Serum AFP was determined by electrochemiluminescence (ECL) (AFP reference value range: <10 ng/mL).Results: There were characteristic changes in the serum N-glycan profiles of patients with HBV-related liver disease, including NA2FB, NA3, and NA3Fb. NA2FB was the most abundant in LC patients, while NA3Fb abundance was the highest in HCC patients. For HCC screening in patients, especially in patients with LC, the sensitive of Log peak 9 (94.4%) and Log (peak 9/peak 7) (88.9%) were better than alphafetoprotein (AFP) (33.3-61.1%), and their specificity was similar to that of AFP. The receiver operating characteristic (ROC) curve showed that the accuracy of Log peak 9 (AUC: 0.81±0.07) and Log (peak 9/peak 7) (AUC: 0.87±0.06) was better than that of AFP (AUC: 0.72±0.09), while the accuracy of AFP combined with the above 2 indexes was better than that of a single index. Moreover, Log (peak 9/peak 7) combined with AFP (AUC: 0.89±0.06) had the best accuracy in the diagnosis of HCC.Conclusions: Our research indicates that N-glycan may serve a new, valuable, and noninvasive alternative method for diagnosing HCC, and it may be a supplement to AFP in the diagnosis of HCC in patients with HBV-related liver disease.

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Serum N‐glycan profiling as a diagnostic biomarker for the identification and assessment of psoriasis

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 8 publications

References 46 publications

N‐glycan profiling alterations of serum and immunoglobulin G in immune thrombocytopenia

N‐glycan profiling alterations of serum and immunoglobulin G in immune thrombocytopenia

Proteomic and Metabolomic Changes in Psoriasis Preclinical and Clinical Aspects

Serum N-glycan profiling as a diagnostic biomarker for the identification of hepatitis B virus-associated hepatocellular carcinoma

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