Serum
            <i>Helicobacter Pylori</i>
            CagA Antibody As A Biomarker For Gastric Cancer in East-Asian Countries

Shiota, Seiji; Matsunari, Osamu; Watada, Masahide; Yamaoka, Yoshio

doi:10.2217/fmb.10.135

Cited by 58 publications

(56 citation statements)

References 57 publications

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“…Faecal-oral transmission is another route of infection related to sanitary conditions; water supply and sewage are considered important factors for H. pylori infection (Goh et al, 2011). After the Second World War, sanitary conditions improved in Japan and the prevalence of H. pylori infection decreased (Shiota et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Analysis of intra-familial transmission of Helicobacter pylori in Japanese families

Osaki

Konno

Yonezawa

et al. 2015

Journal of Medical Microbiology

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Intra-familial infection is considered to be one of the main routes of transmission for Helicobacter pylori in Japan. We assessed the genomic profiles of H. pylori isolates from family members by multi-locus sequence typing (MLST) and identified the original strain infecting the index child. A total of 19 isolates from five families were analysed by MLST using seven housekeeping genes and by random amplification of polymorphic DNA (RAPD)-PCR. Phylogenetic analysis was performed using nucleotide sequences of the seven loci. Two or more different types of H. pylori strains were indicated in three (K-1, K-2 and K-5) out of five families. Independent genotypes of H. pylori strains were detected from all members of the other two families suggesting that these strains (K26-28 and K29-33) may be dominant. Mother-to-child transmission of H. pylori was demonstrated in four out of five families, whilst transmission from father-to-child and sibling-tosibling were demonstrated in two families and one family, respectively.

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Section: Discussionmentioning

confidence: 99%

Analysis of intra-familial transmission of Helicobacter pylori in Japanese families

Osaki

Konno

Yonezawa

et al. 2015

Journal of Medical Microbiology

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“…Moreover, meta-analyses identified a significant relationship between vacA m-region genotype and cagA status and the development of diseases in Southeast Asia. Importantly, most of the H. pylori strains isolated from countries with high incidences of gastric cancer and anti-cagA antibody can be used as a biomarker for gastric cancer even in East Asian countries [55,[77][78].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of cagA and vacA among Helicobacter pylori-infected patients in Iran: a systematic review and meta-analysis

Sayehmiri

Kiani

Sayehmiri

et al. 2015

J Infect Dev Ctries

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The varieties of infections caused by Helicobacter pylori may be due to differences in bacterial genotypes and virulence factors as well as environmental and host-related factors. This study aimed to investigate the prevalence of cagA and vacA genes among H. pylori-infected patients in Iran and analyze their relevance to the disease status between two clinical groups via a meta-analysis method. Different databases including PubMed, ISI, Scopus, SID, Magiran, Science Direct, and Medlib were investigated, and 23 relevant articles from the period between 2001 and 2012 were finally analyzed. The relevant data obtained from these papers were analyzed by a randomeffects model. Data were analyzed using R software and STATA. The prevalence of cagA and vacA genes among H. pylori-infected patients was 70% (95% CI, 64-75) and 41% (95% CI, 24.3-57.7), respectively. The prevalence of duodenal ulcers, peptic ulcers, and gastritis among cagA+ individuals was 53% (95% CI, 20-86), 65% (95% CI, 34-97), and 71% (95% CI, 59-84), respectively. Odds ratio (OR) between cagA-positive compared with cagA-negative patients showed a 1.89 (95% CI, 1.38-2.57) risk of ulcers. In conclusion, the frequency of cagA gene among H. pylori strains is elevated in Iran and it seems to be more frequently associated with gastritis. Therefore, any information about cagA and vacA prevalence among different H. pylori-infected clinical groups in the country can help public health authorities to plan preventive policies to reduce the prevalence of diseases associated with H. pylori infection.

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“…On the other hand, there were no false positive results and therefore the Western-rCagA showed 100% specificity. This is also a good result for the rCagA of this study because the lack of specificity of antiCagA tests has been attributed in part to differences in the CagA antigens used (12,30).…”

Section: Discussionmentioning

confidence: 56%

“…However, the accuracy of the tests developed with this regard has been questioned because the results differed when they were used in different populations. It has been suggested that an important issue to this heterogeneity appeared to result from differences in the antigens used to detect anti-CagA antibodies (12). Consequently, new studies using the already existent CagA antigens, and/or some new design fragments of the cytotoxin, should be done to evaluate the reactivity of representative populations to different antigens.…”

Section: Discussionmentioning

confidence: 99%

Clonaje y expresión de un fragmento recombinante del gen cagA de Helicobacter pylori y su evaluación preliminar en el serodiagnóstico

et al. 2013

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Introduction:Helicobacter pylori strains expressing cytotoxic CagA protein are more commonly associated with peptic ulceration, atrophic gastritis and gastric adenocarcinoma than those lacking CagA. Determination of anti-CagA antibodies, therefore, acquires a relevant clinical significance in the serological detection of H. pylori infection and disease risk prediction. However, the CagA-serology has been questioned due to the differences found in their performance evaluations in different populations. Objective: To obtain a recombinant CagA fragment useful for serodiagnosis of H. pylori infection Methods: A fragment of the cagA gene was cloned into a prokaryotic T7 RNA polymerase expression vector. A recombinant C-terminal His 6 -tagged CagA was expressed, subsequently solubilized with urea and purified by immobilized metal affinity chromatography. The performance of the recombinant protein was evaluated using 180 human serum samples with an in-house Western blot assay compared to the Helicoblot 2.1 reference test. Results: The expressed His 6 -tagged CagA showed an immunoreactive 80kDa band as was revealed by SDS-PAGE and Western blot analysis using two different specific anti-CagA polyclonal antibodies. The recombinant protein was successfully purified obtaining a 93% of purity. The performance analysis of the purified recombinant antigen showed good immunoreactivity and exhibited values of sensitivity, specificity and accuracy of 88.1%, 100% and 92.7%, respectively. Conclusion: The CagA fragment of the study may constitute a useful tool for serological diagnosis of CagA-positive H. pylori infection. Clonación y expresión de un fragmento recombinante del gen cagA de Helicobacter pylori y su evaluación preliminar en el serodiagnóstico Introducción. Las cepas de Helicobacter pylori que expresan la citotoxina CagA, se asocian más frecuentemente con úlcera péptica, gastritis atrófica y adenocarcinoma gástrico que las que carecen de esta citotoxina. Por lo anterior, el determinar la presencia de anticuerpos anti-CagA adquiere gran importancia clínica en la detección serológica de la infección por H. pylori y la predicción del riesgo de enfermedades. Sin embargo, los métodos serológicos que emplean CagA han sido cuestionados debido a las diferencias encontradas en las evaluaciones de su desempeño en diversas poblaciones. Objetivo. Obtener un fragmento recombinante de la proteína CagA para el serodiagnóstico de la infección por H. pylori. Materiales y métodos. Un fragmento del gen cagA fue clonado en un vector de expresión procariota que contenía el promotor de la T7 ARN polimerasa. El fragmento de la proteína CagA con seis histidinas en la región C-terminal, se expresó, se solubilizó con urea y se purificó por cromatografía de afinidad con iones metálicos inmovilizados. El desempeño de la proteína recombinante se evaluó empleando un método in house de Western Blot y 180 sueros humanos. Los resultados se compararon con la prueba de referencia Helicoblot 2.1. 547 Biomédica 2013;33:546-53 Recombinant CagA fragment fo...

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Serum Helicobacter Pylori CagA Antibody As A Biomarker For Gastric Cancer in East-Asian Countries

Cited by 58 publications

References 57 publications

Analysis of intra-familial transmission of Helicobacter pylori in Japanese families

Analysis of intra-familial transmission of Helicobacter pylori in Japanese families

Prevalence of cagA and vacA among Helicobacter pylori-infected patients in Iran: a systematic review and meta-analysis

Clonaje y expresión de un fragmento recombinante del gen cagA de Helicobacter pylori y su evaluación preliminar en el serodiagnóstico

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