Serum Human Telomerase Reverse Transcriptase Messenger RNA as a Novel Tumor Marker for Hepatocellular Carcinoma

Miura, Naruhisa; Maeda, Yoshiko; Kanbe, Takamasa; Yazama, Hiroaki; Takeda, Yohei; Sato, Reina; Tsukamoto, Tomoe; Sato, Emi; Marumoto, Akira; Harada, Tomonari; Sano, Akiko; Kishimoto, Yosuke; Hirooka, Yasuaki; Murawaki, Yoshikazu; Hasegawa, Junichi; Shiota, Goshi

doi:10.1158/1078-0432.ccr-04-1487

Cited by 74 publications

(63 citation statements)

References 23 publications

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“…It has been reported that the sensitivity and specificity of hTERT mRNA in detecting HCC are 88.2% and 70.0%, respectively, which excel those of conventional tumor markers, such as AFP mRNA, AFP and DCP [50] . Moreover, it has been indicated that the expression of serum hTERT mRNA, which is associated with the serum concentration of AFP, tumor size, and tumor differentiation degree (P < 0.001, each), may be a valuable indicator of poor prognosis for HCC patients [50,51] . There are some other markers in this category, which could be used as diagnostic or prognostic indicators for HCC.…”

Section: Genesmentioning

confidence: 99%

“…Furthermore, it has also been demonstrated to be a novel and available marker for HCC diagnosis. The expression of hTERT mRNA in the serum of HCC patients is significantly higher than that in the serum of healthy adults or patients with nonmalignant hepatopathy [50,51] , and the use of newly developed real-time quantitative reverse transcription polymerase chain reaction may improve the effectiveness of determination [50] . It has been reported that the sensitivity and specificity of hTERT mRNA in detecting HCC are 88.2% and 70.0%, respectively, which excel those of conventional tumor markers, such as AFP mRNA, AFP and DCP [50] .…”

Section: Genesmentioning

confidence: 99%

“…The expression of hTERT mRNA in the serum of HCC patients is significantly higher than that in the serum of healthy adults or patients with nonmalignant hepatopathy [50,51] , and the use of newly developed real-time quantitative reverse transcription polymerase chain reaction may improve the effectiveness of determination [50] . It has been reported that the sensitivity and specificity of hTERT mRNA in detecting HCC are 88.2% and 70.0%, respectively, which excel those of conventional tumor markers, such as AFP mRNA, AFP and DCP [50] . Moreover, it has been indicated that the expression of serum hTERT mRNA, which is associated with the serum concentration of AFP, tumor size, and tumor differentiation degree (P < 0.001, each), may be a valuable indicator of poor prognosis for HCC patients [50,51] .…”

Section: Genesmentioning

confidence: 99%

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Serum tumor markers for detection of hepatocellular carcinoma

Zhou¹

2006

WJG

300

259

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Alpha-fetoprotein and alpha-fetoprotein-L3Alpha fetoprotein (AFP) is a fetal specific glycoprotein produced primarily by the fetal liver. Normally, its serum concentration falls rapidly after birth and its synthesis in adult life is repressed. However, greater than 70% of HCC patients have a high serum concentration of AFP because of the tumor excretion. Forty years after its discovery, serum AFP remains a most useful tumor marker in screening HCC patients. The serum concentration of 20 ng/mL is the most commonly used cut-off value to differentiate HCC patients from healthy adults in clinical researches. However, some investigations have showed that the cut-off value is fluctuant in different ethnic groups. REVIEW Serum tumor markers for detection of hepatocellular carcinoma AbstractHepatocellular carcinoma (HCC) is one of the most frequent malignant tumors and is the second most common cause of cancer death in China. Therefore, it is very important to detect this disease and the recurrence at its earlier period. Serum tumor markers, as the effective method for detecting hepatocellular carcinoma for a long time, could be divided into 4 categories: oncofetal antigens and glycoprotein antigens; enzymes and isoenzymes; genes; and cytokines. Serum alpha fetoprotein (AFP) is the most widely used tumor marker in detecting patients with hepatocellular carcinoma, and has been proven to have capability of prefiguring the prognosis. However, it has been indicated that AFP-L3 and DCP excel AFP in differentiating hepatocellular c a r c i n o m a f r o m n o n m a l i g n a n t h e p a t o p a t h y a n d detecting small hepatocellular carcinoma. Some tumor markers, such as human cervical cancer oncogene and human telomerase reverse transcriptase mRNA, have also been indicated to have higher accuracies than AFP. Furthermore, some other tumor markers, such as glypican-3, gamma-glutamyl transferase II, alpha-lfucosidase, transforming growth factor-beta1, tumorspecific growth factor, have been indicated to be available supplementaries to AFP in the detection. AFP mRNA has been shown to correlate with the metastasis and recurrence of HCC, and it may be the most useful marker to prefigure the prognosis. Some other markers, such as gamma-glutamyl transferase mRNA, vascular endothelial growth factor, and interleukin-8, could also be used as available prognostic indicators, and the simultaneous determination of AFP and these markers may detect the recurrence of HCC at its earlier period.

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Section: Genesmentioning

confidence: 99%

Section: Genesmentioning

confidence: 99%

Section: Genesmentioning

confidence: 99%

See 1 more Smart Citation

Serum tumor markers for detection of hepatocellular carcinoma

Zhou¹

2006

WJG

300

259

View full text Add to dashboard Cite

show abstract

“…Serum tumor markers, such as carcinoembryonic antigen (cea) and carbohydrate antigen (ca) 19-9, have been widely used as convenient diagnostic assays, but their lack of sufficient sensitivity and specificity for the early detection of (14,15). Several studies have determined that tumor-associated circulating cell-free mrna in the plasma or serum is increased in cancer patients (16)(17)(18)(19)(20). at least some of these nucleic acids are released from tumor cells and can be considered new non-invasive biomarkers for diagnosing disease or predicting patient prognosis (21).…”

Section: Introductionmentioning

confidence: 99%

Combination of circulating CXCR4 and Bmi-1 mRNA in plasma: A potential novel tumor marker for gastric cancer

Zhang

Qian

et al. 2009

Mol Med Rep

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Abstract. cell-free nucleic acids in plasma have been reported to be a novel tumor marker. However, their exact significance in gastric cancer is unclear. in the present study, we focused on circulating CXCR4 and Bmi-1 mrna in plasma and evaluated their diagnostic values for patients with gastric cancer. CXCR4 and Bmi-1 mrna levels, as well as levels of cea and ca19-9 proteins, were determined in the plasma of 89 gastric cancer patients. The levels of these markers were significantly higher in cancer patients than in healthy subjects; however, there was a decrease in their expression after surgery. The sensitivity of detection for the combination of circulating CXCR4 and Bmi-1 mrna was higher than the sensitivities of detection for cea and ca19-9. in patients expressing both genes, CXCR4 and Bmi-1 mrna levels were strongly correlated. expression of the two circulating genes was also strongly correlated with the CEA level, and was significantly associated with the age of the patient, histological type, clinical stage, extent of cell differentiation and gastric cancer metastasis. our results indicate that the combined expression of circulating CXCR4 and Bmi-1 mrna represents a novel tumor marker, superior to traditional markers such as cea and ca19-9, for the diagnosis and monitoring of gastric cancer. IntroductionGastric cancer is one of the most lethal malignant tumors worldwide, with a 5-year survival rate of only 30-40% in patients with aggressive-phase disease. lymph node metastases are found in most patients with advanced gastric cancer. However, treatment of early-stage disease results in higher survival rates (1). Thus, the early detection of gastric cancer and prediction of the metastatic potential of the tumor are necessary to improve the survival rate.it has been reported that the chemokine cXc receptor 4 (cXcr4) and the cXcl12 ligand are involved in the metastasis of various types of cancer, including gastric carcinoma (2). The CXCR4 gene is located on the long arm of chromosome 2 and encodes 352 amino acids. The protein is a seven-transmembrane domain-containing G protein-coupled receptor, and is the unique high-affinity receptor for CXCL12 (3). It has been demonstrated that cancer cells express chemokine receptors, and that their corresponding ligands are frequently expressed at the site of tumor metastasis (4,5). Muller et al revealed that CXCR4 was highly expressed in human breast cancer cells and their distal metastases, while CXCL12 expression was increased at the common sites of breast cancer metastasis, such as the lymph nodes, lungs and liver (6). This indicates that CXCR4 is a potential tumor marker for predicting the locations and probability of tumor metastasis.The Bmi-1 gene is a member of the polycomb family that is located on the short arm of chromosome 10 and encodes a 326-amino acid protein. By deregulating two tumor suppressors, p16inK4a and p19arF (p14arF in humans), Bmi-1 can inhibit the apoptosis or senescence induced by p53 and prb (7,8). Furthermore, Bmi-1 plays a role in the act...

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“…The biological roles of circulating mRNA are still unclear, although its physiological significance has been investigated during the last several years [14]. Recently, the plasma circulating mRNA in cancer patients [7] and pregnant women [8] has been detected and analyzed with respect to sensitivity and specificity. These studies successfully demonstrated the clinical values of the detection for early cancer diagnosis and monitoring, and prenatal diagnosis of abnormal development.…”

mentioning

confidence: 99%

Utility of Plasma Circulating mRNA as a Marker to Detect Hepatic Injury

Kudo

Ochi

Shimada

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Utility of plasma circulating mRNA as a molecular marker to detect hepatic injury was evaluated. Total RNA was isolated from plasma of the rat liver fibrosis models at various time points, and plasma circulating mRNAs of major liver-derived genes, albumin and haptoglobin, were measured by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). Sensitivity and kinetics of plasma circulating mRNA were compared with those of plasma alanine aminotransferase (ALT) activity. We have found that the measurement of plasma circulating mRNA is more sensitive than plasma ALT activity, and enables early detection of hepatic injury. The plasma circulating mRNA will serve as a novel and highly sensitive molecular marker for hepatic injury. KEY WORDS: alanine aminotransferase, hepatic injury, plasma circulating mRNA.

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Serum Human Telomerase Reverse Transcriptase Messenger RNA as a Novel Tumor Marker for Hepatocellular Carcinoma

Cited by 74 publications

References 23 publications

Serum tumor markers for detection of hepatocellular carcinoma

Serum tumor markers for detection of hepatocellular carcinoma

Combination of circulating CXCR4 and Bmi-1 mRNA in plasma: A potential novel tumor marker for gastric cancer

Utility of Plasma Circulating mRNA as a Marker to Detect Hepatic Injury

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