Serum HLA class I antigens: markers and modulators of an immune response?

Puppo, Francesco; Scudeletti, Marco; Indiveri, Francesco; Ferrone, Soldano

doi:10.1016/0167-5699(95)80127-8

Cited by 117 publications

(97 citation statements)

References 23 publications

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“…Among them is the gender of the donors, since the level of sHLA-G is higher in women than in men [39]. Furthermore, as previously observed for classical HLA class I antigens (18,19), another important variable is represented by the HLA-G polymorphism. Thus, healthy individuals carrying the HLA-G*01013 allele or the "null" allele HLA-G*0105N have significantly lower sHLA-G levels than subjects carrying the more frequent HLA-G*01011 and HLA-G*01012 alleles.…”

Section: Expression Of Shla-g In Physiological Conditionsmentioning

confidence: 57%

“…sHLA-G has been described thus far in serum, in amniotic fluid and in CSF. Whether it is also present in urine, tears and milk, like classical HLA class I antigens (18,19), remains to be determined. Several variables have been found to influence the level of sHLA-G in serum.…”

Section: Discussionmentioning

confidence: 99%

“…Like other types of histocompatibility antigens (18,19), HLA-G are expressed not only on the cell surface, but also in body fluids. sHLA-G has been described thus far in serum, in amniotic fluid and in CSF.…”

Section: Discussionmentioning

confidence: 99%

“…Like classical sHLA class I antigens (18,19), sHLA-G1 inhibits the cytotoxic activity of HLA class I antigen restricted, antigen-specific CTL [9,30,32] sHLA-G5 also inhibits CD4 and CD8 T cell alloproliferation by blocking cell cycle progression. However sHLA-G5 does not induce apoptosis of alloreactive T cells [12].…”

Section: Molecular and Functional Characteristics Of Shla-gmentioning

confidence: 99%

“…Like other types of histocompatibility antigens (18,19), HLA-G are expressed not only on the surface membrane of cells, but also in body fluids. In the present paper we will review the molecular and functional characteristics of sHLA-G, their expression in physiological and pathological conditions and their potential clinical relevance.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Soluble HLA-G: Are they clinically relevant?

Pistoia¹,

Morandi²,

Wang³

et al. 2007

Seminars in Cancer Biology

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161

182

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HLA-G is a non-classical HLA-class Ib molecule with multiple immunoregulatory properties. Its main function in physiological conditions is to abrogate maternal NK cell activity against foetal tissue and to establish immune tolerance at maternal-foetal interface. HLA-G is expressed not only as a membrane bound molecule on the surface of cells, but also as a soluble moiety in body fluids. The major isoforms of HLA-G present in serum are soluble HLA-G1 and HLA-G5 which are generated by shedding or proteolytic cleavage of the membrane bound isoform and by secretion of a soluble isoform, respectively.Here we review the data about soluble HLA-G (sHLA-G) serum levels in different pathological conditions, including immune-mediated disorders, transplantation, and malignancies. In particular, we focus on sHLA-G expression and function in human neuroblastoma, a pediatric tumor, with special emphasis on a novel potential immuno escape mechanism utilized by NB to instruct monocytes to produce and release sHLA-G. Finally, the potential clinical relevance of sHLA-G serum levels is discussed.

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Section: Expression Of Shla-g In Physiological Conditionsmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Molecular and Functional Characteristics Of Shla-gmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Soluble HLA-G: Are they clinically relevant?

Pistoia¹,

Morandi²,

Wang³

et al. 2007

Seminars in Cancer Biology

Self Cite

161

182

View full text Add to dashboard Cite

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Quantitative cytometry of MHC class I digestion from living cells

Galati¹,

Arcelloni²,

Paroni³

et al. 1997

Cytometry

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Digestion of crude membrane preparations with papain releases the extracellular portion of major histocompatibility complex (MHC) class I molecules. MHC class I molecules are integral membrane glycoprotein complexes formed by the noncovalent association of 2 invariant molecules, the heavy chain and the β2‐microglobulin (β2‐m), to a wide array of peptides. The cleaved soluble moiety retains the antigenic properties of the intact membrane‐bound complex. Here we show that MHC class I digestion may be carried out on living cells, and we quantitate the surface expression of MHC complexes by a combined cytometric/high performance liquid chromatographic (HPLC) approach. Papain digestion results in time‐ and dose‐dependent disappearance of membrane MHC‐associated‐fluorescence as detected by FACS analysis with MHC‐specific monoclonal antibodies (mAbs). β2‐m and peptides became detectable by HPLC analysis and western blotting in the digestion buffer and were quantitated by comparison with purified standards. The cytometric assessment of the digestion allows one to simultaneously monitor efficacy and toxicity of the treatment. The procedure we describe allows to selectively retrieve by affinity chromatography MHC from the cell membrane, avoiding any contamination due to intracellular, “immature” MHC molecules. Cytometry 27:77–83, 1997. © 1997 Wiley‐Liss, Inc.

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Early Conceptus Growth and Immunobiologic Adaptations of Pregnancy

and

Adashi

2007

Clinical Obstetrics

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Serum HLA class I antigens: markers and modulators of an immune response?

Cited by 117 publications

References 23 publications

Soluble HLA-G: Are they clinically relevant?

Soluble HLA-G: Are they clinically relevant?

Quantitative cytometry of MHC class I digestion from living cells

Early Conceptus Growth and Immunobiologic Adaptations of Pregnancy

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