Serum Hepatitis B Virus DNA in Acute Hepatitis Type B

Pontisso, Patrizia; Bortolotti, Flavia; Schiavon, E; Chemello, Liliana; Alberti, Alfredo; Realdi, Giuseppe

doi:10.1159/000199309

Cited by 16 publications

(5 citation statements)

References 4 publications

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“…On the other hand, antibodies against the pHSA receptor which have been shown in serum during acute hepatitis B may have an important role for viral clearance (Alberti et al, 1984;Pontisso et al, 1983b). As a result, it would be useful to use HBsAg particles carrying the pHSA receptor in vaccination programs (Michel et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo synthesis of the hepatitis B virus surface antigen and of the receptor for polymerized human serum albumin from recombinant human adenoviruses.

Ballay¹,

Levrero²,

Buendía³

et al. 1985

The EMBO Journal

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We have developed an adenovirus vector to express foreign proteins under the control of the adenovirus Ela promoter. Two recombinant plasmids, harbouring either the S gene or the pre-S2 region and the S gene of hepatitis B virus under the control of the Ela promoter, were used to construct two recombinant adenoviruses. These two viruses direct the synthesis of hepatitis B virus surface antigen (HBsAg) particles during the time course of an infectious cycle. When the pre-S2 region is present in the constructed virus, the synthesis of particles carrying the receptor for polymerized human serum albumin (pHSA) is observed. Moreover, the inoculation of rabbits with this latter purified recombinant adenovirus elicits the production of antibodies that react with both HBsAg and pHSA receptor.

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo synthesis of the hepatitis B virus surface antigen and of the receptor for polymerized human serum albumin from recombinant human adenoviruses.

Ballay¹,

Levrero²,

Buendía³

et al. 1985

The EMBO Journal

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“…The appearance of antibodies to pHSA receptor in the serum is observed during recovery from hepatitis B, characterized by the seroconversion HBeAg/anti-HBe antibodies, but never during the evolution toward chronicity (5,6). This suggests that antibodies to pHSA receptor are important for viral clearance.…”

mentioning

confidence: 99%

Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin.

Michel¹,

Pontisso²,

Sobczak³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

173

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“…Serum HBV-DNA Serum HBV-DNA was studied according to a method previously described [12,13,17], Briefly, 100 pi of denaturated serum were spotted under vacuum on nylon membranes, fixed at 80 °C for 2 h and hybrid ized with an HBV-DNA insert radiolabeled with 32P. The probe, kindly provided by C. Brechot (Pasteur Institute, Paris, France), was purified from the cloning vector (pBR 322) by electrophoresis after EcoRI diges tion.…”

Section: Liver Hbcagmentioning

confidence: 99%

Cryptogenic Chronic Liver Disease and Serum or Liver Hepatitis B Virus Markers

Diodati¹,

Pontisso²,

Bonetti³

et al. 1988

Digestion

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In an attempt to evaluate a possible correlation between cryptogenic chronic liver disease and a present or past hepatitis B virus (HBV) infection, we studied 17 patients with hepatitis B surface antigen (HB_sAg)-negative, nonalcoholic chronic liver disease; 9 of them were positive for serum HB_sAg detected by a solid-phase enzyme immunoassay with monoclonal antibody (M-EIA) and 8 were negative for the same marker. Liver hepatitis B core antigen (HB_cAg), studied by an indirect immunofluorescence technique, was present in 55.5% of the patients positive for serum HB_sAg by M-EIA. In the same group of patients, liver HBV-DNA was found in 66.6% of the patients. On the other hand, only 1 patient without serum positivity for HB_sAg by M-EIA was positive for liver HB_cAg and HBV-DNA. None of our patients showed serum positivity for HBV-DNA sequences. We conclude that HBV infection may be a possible cause of cryptogenic chronic liver disease; this HBV-related, HB_sAg-negative chronic liver disease seems to have no viral replication or undetectable levels of HBV-DNA in serum. HB_sAg, detected by a monoclonal assay, seems to be a suitable marker to identify this subgroup of patients with HB_sAg-negative chronic liver disease.

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Serum Hepatitis B Virus DNA in Acute Hepatitis Type B

Cited by 16 publications

References 4 publications

In vitro and in vivo synthesis of the hepatitis B virus surface antigen and of the receptor for polymerized human serum albumin from recombinant human adenoviruses.

In vitro and in vivo synthesis of the hepatitis B virus surface antigen and of the receptor for polymerized human serum albumin from recombinant human adenoviruses.

Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin.

Cryptogenic Chronic Liver Disease and Serum or Liver Hepatitis B Virus Markers

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