Serum haptoglobin concentrations in concurrent hemolysis and acute-phase reaction.

Warkentin, Donald L.; Marchand, Anthony; Lente, Frederick Van

doi:10.1093/clinchem/33.7.1265

Cited by 14 publications

(4 citation statements)

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“…This finding is of particular importance, because it had been assumed that acute‐phase‐driven stimulation of Hp synthesis would counterbalance Hp depletion in haemolysis [7,9]. To the best of our knowledge, there has been only one study on this issue reporting haemolysis‐induced Hp depletion apparently not counterbalanced by an acute‐phase reaction in surgery patients [28]. The provided level of evidence, however, appears to be limited because in that study Hp depletion was recorded only on the first postoperative day, probably before Hp synthesis was increased by surgery‐induced inflammation.…”

Section: Discussionmentioning

confidence: 99%

Influence of clinical factors on the haemolysis marker haptoglobin

Körmöczi

Säemann

Buchta

et al. 2006

Eur J Clin Investigation

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Section: Discussionmentioning

confidence: 99%

Influence of clinical factors on the haemolysis marker haptoglobin

Körmöczi

Säemann

Buchta

et al. 2006

Eur J Clin Investigation

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“…Serum haptoglobin concentrations significantly decreased after surgery in 56 of 58 patients. Of those patients, 41 (70.7%) had haptoglobin less than 25 mg/dl . Therefore, haptoglobin testing is theoretically less reliable for the detection of hemolysis during periods of inflammation, and individual results should be interpreted in the clinical context.…”

Section: Limitationsmentioning

confidence: 99%

Haptoglobin testing in hemolysis: Measurement and interpretation

2014

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Haptoglobin is primarily produced in the liver and is functionally important for binding free hemoglobin from lysed red cells in vivo, preventing its toxic effects. Because haptoglobin levels become depleted in the presence of large amounts of free hemoglobin, decreased haptoglobin is a marker of hemolysis. Despite its ubiquity and importance, a paucity of literature makes testing difficult to interpret. This review highlights the many physiological roles that have been recently elucidated in the literature. Different methodologies have been developed for testing, including spectrophotometry, immunoreactive methods, and gel electrophoresis. These are covered along with their respective advantages and disadvantages. As there is no single gold standard for hemolysis, validation studies must rely on a combination of factors, which are reviewed in this article. Pitfalls and limitations of testing are also addressed. False positives can occur in improper specimen preparations, cirrhosis, elevated estrogen states, and hemodilution. False negatives can occur in hypersplenism and medications such as androgens and corticosteroids. Haptoglobin testing in the setting of inflammation is additionally discussed as interpretation can be difficult in this setting. Given the widespread use of haptoglobin testing, it is vital that clinicians and laboratory staff understand the principles and correct interpretation of this test.

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“…Therefore, serum Hp level is often used as a diagnostic marker for hemolytic processes. [14][15][16][17][18]…”

Section: Introductionmentioning

confidence: 99%