Serum haemolytic and agglutinating activity as indicators of fish immunocompetence: their suitability in stress and dietary studies

Tort, L.; Gómez, E.; Montero, Daniel; Sunyer, J. Oriol

doi:10.1007/bf00175219

Cited by 117 publications

(75 citation statements)

References 22 publications

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“…In other teleosts, such as Sparus aurata [8,55,56], Salmo trutta [6] and Oreochromis niloticus [57] the same time period is sufficient to observe a return to normality.…”

Section: Discussionmentioning

confidence: 99%

“…With respect to the haemagglutinating activity, agglutinins are recognised to be a key component of innate immune system in many fish species, which are active against homologous or heterologous red blood cells [8,10].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, some components of innate immune system (e.g. lysozyme, haemolytic and haemagglutinating activity) are used as indicators of immunocompetence in fish exposed to stress [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Maricchiolo¹,

Caruso²,

Genovese³

2008

TOFISHSJ

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Abstract:The physiological effects of acute stress (induced by an intraperitoneal injection) on the haematocrit, haemoglobin, serum cortisol and glucose, haemagglutinins and lysozyme were investigated in juvenile sea bass Dicentrarchus labrax. A total of 40 fish were subjected to an intraperitoneal injection. Eight fish were sampled at 0, 2, 4, 24 and 48 h after acute stress and compared to unstressed fish (controls) sacrificed at the same times. Intraperitoneal injection resulted in significant changes in cortisol and glucose 4 and 2 hours after exposure to stress, respectively, but not in haematocrit and haemoglobin levels. No significant differences between stressed and control fish were recorded for lysozyme content and haemagglutinating activity throughout the experiment. In juvenile sea bass acute stress caused by intraperitoneal injection was mostly associated with the increase in common stress indicators (cortisol and glucose), while no clear response in terms of non-specific immune parameters was evidenced.

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“…In other teleosts, such as Sparus aurata [8,55,56], Salmo trutta [6] and Oreochromis niloticus [57] the same time period is sufficient to observe a return to normality.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Maricchiolo¹,

Caruso²,

Genovese³

2008

TOFISHSJ

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“…Circulating leucocyte counts, often used as a parameter to assess the immunological health of fish, have been shown here not to accurately reflect the real health status of the fish because circulating levels of leucocytes are maintained by the immunological organs. Other studies in our laboratory have shown that in gilthead sea bream differences in circulating lymphocyte numbers were noted between 3 and 9 d after acute or chronic disturbance (Sufier & Tort 1995, Suner et al 1995, Tort et al 1996.…”

Section: Discussionmentioning

confidence: 79%

Handling, confinement and anaesthetic exposure induces changes in the blood and tissue immune characteristics of gilthead sea bream

Cubero¹,

Molinero²

1997

Dis. Aquat. Org.

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The gilthead sea bream Sparus aurata is a species of great ~nterest for aquaculture, and in the last few years its culture has increased in the Mediterranean. This study was carried out to elucidate the mechanisms underlying the immunological response of fish after handling and confinement, procedures commonly associated with fish transport, as well as to determine the putative protective role of an anaesthetic (tricaine methanosulphonate) during confinement. Handling produces changes not only in circulating blood cells but also in the immunological cell populations of the thymus, spleen and pronephros. A stronger response in immunological cells was obtained when handllng was followed by confinement. Circulating white blood cells returned to normal approximately 48 h after the onset of stress, whereas immunological tissue cells recovered later. The presence of an anaesthetic partially prevented the circulatory response, suggesting that the immunological response was less and supporting therefore the belief that the anaesthetic plays a protective role. However, it worsened the effect of handling plus confinement on haematopoietic organs, indicating that examining immunological cells in circulation only can lead to a false conclusion. Our results suggest that fish are not completely recovered until at least 144 h (6 d) after handling and transport, when cellular recovery in immunological organs occurs.

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“…Mean corpuscular hemoglobin content (MCHC) was calculated according to the formula: MCHC = Hb (g/dl)/ Hk. Plasma from these fresh blood samples was used to analyze alternative complement activity (ACH50) following Tort et al (1996). Plasma from the remaining five blood samples from each tank were used in the lysozyme analysis following the procedures of Hutchinson and Manning (1996) and Magnadottir et al (1999).…”

Section: Feeding and Monitoringmentioning

confidence: 99%

A dietary dairy/yeast prebiotic and flaxseed oil enhance growth, hematological and immunological parameters in channel catfish at a suboptimal temperature (15°C)

Thompson

Lochmann

Phillips

et al. 2015

Animal

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Channel catfish raised in the southern United States require two growing seasons to reach market size. Growing seasons are separated by a cool period of about 3 months when feed intake and growth are greatly reduced. A cool-weather feeding strategy to improve feed intake, growth or health of catfish might improve survival and reduce the time needed to achieve market size. We conducted a feeding trial with channel catfish at a suboptimal temperature (15°C) to determine the effects of supplementing diets with either a dairy/yeast prebiotic or flaxseed oil (high in 18:3n-3) compared with a control with soybean oil (high in 18:2n-6). The trial was conducted in recirculating systems with 1140-l tanks containing 100 fish each (mean initial weight 61.4 g ± 0.43 s.e.m.). A 28%-protein basal diet was supplemented with 20 g/kg cellulose and 20 g/kg soybean oil (SBO, control), 20 g/kg cellulose and 20 g/kg flaxseed oil (FLAX) or 20 g/kg of a dairy/yeast prebiotic and 20 g/kg soybean oil (PREB). Fish were fed once daily to satiation and weighed every 3 weeks to track growth. Hematology, non-specific immune responses, proximate and fatty acid composition of muscle were determined to assess diet effects. Catfish-fed FLAX or PREB had higher weight gain, feed consumption and lysozyme activity than fish fed SBO. Total n-3 fatty acids in muscle were higher in fish fed SBO or FLAX than those fed PREB. Total n-6 long-chain polyunsaturated acids were higher in muscle of fish fed PREB than those fed SBO. Fatty acids in the PREB and SBO diets were similar, so the PREB appeared to increase elongation and desaturation of n-6 fatty acids in muscle. Flaxseed oil and the dairy/yeast prebiotic both have potential to increase catfish performance at a low temperature.

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Serum haemolytic and agglutinating activity as indicators of fish immunocompetence: their suitability in stress and dietary studies

Cited by 117 publications

References 22 publications

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Handling, confinement and anaesthetic exposure induces changes in the blood and tissue immune characteristics of gilthead sea bream

A dietary dairy/yeast prebiotic and flaxseed oil enhance growth, hematological and immunological parameters in channel catfish at a suboptimal temperature (15°C)

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