Serum group I pepsinogen levels in patients with peptic ulcer and normal subjects.

Nakanome, Chiyuki; Akai, Hiroaki; Gotō, Yoshio

doi:10.1620/tjem.139.151

Cited by 11 publications

(10 citation statements)

References 15 publications

(5 reference statements)

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“…In this study, both serum pepsinogens A and C were increased in duodenal ulcer disease, äs was previously reported for serum pepsinogen A (10,15,16,20,21) and serum pepsinogen C (10,20). In contrast to other studies (10,20), we observed a significant reduction of the pepsinogen A to pepsinogen C ratio in these patients.…”

Section: Discussionsupporting

confidence: 56%

Radioimmunoassay of Human Pepsinogen A and Pepsinogen C

Biemond¹,

Jansen²,

Crobach³

et al. 1989

Clinical Chemistry and Laboratory Medicine

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Summary:We describe the development of radioimmunoassays to measure both human pepsinogen A and pepsinogen C concentrations in serum. The antibodies were raised in goats by immunization with purified pepsinogen A or C. The affinity constants of the respective antibodies were 20 · l O 10 1/mol and 7 · l O 10 1/mol.Pepsinogens A and C were labeled with Na 125I by the chloramine T method. The binding between labels and antibodies was inhibited by 0.50 at 0.82 ng pepsinogen A per tube and 2.1 ng pepsinogen C per tube. The detection limits of the assay of pepsinogen A and C were 0.12 μg/l and 1.8 μg/l, respectively.Pepsinogen A and C were purified and added to a patient serum, showing a good recovery in the radioimmunoassays. Serial dilution of another patient serum, which contained a high concentration of both antigens, showed curves parallel to the Standard curves. The intra-and interassay variations of these radioimmunoassays were evaluated. The intra-assay coefficients of Variation for pepsinogen A were found to vary from 0.03 to 0.102 at concentrations in serum in the normal r nge, while the inter-assay coefficient of Variation ranged from 0.118 to 0.194 at the same concentrations in serum. For the pepsinogen C radioimmunoassay we found intra-assay coefficients of Variation between 0.126 and 0.147 at concentrations in serum in the normal r nge, while the inter-assay coefficient of Variation ranged from 0.174 to 0.325 for the same sera.In 201 blood donors we found a me n serum concentration of pepsinogen A of 59 μg/l and a mean serum concentration of pepsinogen C of 15 μg/l. There was a significant relationship between these values (r = 0.779, p < 0.001).The concentrations of both pepsinogens were increased in 10 patients with duodenal ulcer (132 and 67 μg/l, respectively). Serum pepsinogen A was decreased in 14 patient« with partial gastrectomy (34 μg/l) and in 6 with achlorhydria (9 μg/l), accompanied by raised (34 μg/l) and!inormal (20 μg/l) mean pepsinogen C concentrations in the respective groups. In 4 patients with a total gastrectomy the mean serum concentration of both pepsinogen A and C reached the zero level (5 and 4 μg/l, respectively). These results not only underline the accuracy of the measurements but also point to their clinical relevance. ntro uction. § secrete^ by f un( jj c , pyloric and proximal duodenal Pepsinogen A and C are the proenzymes of pepsin A mucosa (1). Exocrine-secreted pepsinogens function and pepsin C, respectively, which belong to the group s zymogens for the main proteolytic activity of gasof gastric acid aspartic endoproteiiiases. Pepsinogen tric juice. There is also a small endocrine excretion of A is secreted by fundic mucosa, while pepsinogen C pepsinogens without a known function, but by exploiting this phenomenon, we can gain Informatioñ on the condition of the gastric mucosa by measurePepsin* *Α; EC 3 4.23.1 ment of the serum concentration of pepsinogen A and Pepsin C; gastricsin; EC 3.4.23.3 pepsinogen C (2, 3).

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Section: Discussionsupporting

confidence: 56%

Radioimmunoassay of Human Pepsinogen A and Pepsinogen C

Biemond¹,

Jansen²,

Crobach³

et al. 1989

Clinical Chemistry and Laboratory Medicine

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“…Previous studies have shown that the serum PG I level and/or I/II ratio reflect the morphological (Samloff et al 1982;Borch et al 1989) and functional statuses (Samloff et al 1975;Nakanome et al 1983;Miki et al 1987;Feldman et al 1988) of the gastric mucosa. Thus, many studies have reported a significant correlation between serum PG values and gastric acid secretion levels (Samloff et al 1975;Nakanome et al 1983;Miki et al 1987;Haruma et al 1993;Kinoshita et al 1997;Feldman et al 1988;Iijima et al 2005;Derakhshan et al 2006), although none has demonstrated specific cutoff serum PG values for individual gastric acid secretion levels, which would help estimate the relative risk for various upper GI diseases.…”

Section: Introductionmentioning

confidence: 99%

Cutoff Serum Pepsinogen Values for Predicting Gastric Acid Secretion Status

Iijima

Koike

Abe

et al. 2014

Tohoku J. Exp. Med.

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Measurement of the gastric acid secretion is useful for estimating the risk for various diseases in the upper gastro-intestinal tract; however, the procedure causes significant distress to the subjects. Pepsinogens I and II are secreted from the gastric fundic glands, and thus, the serum pepsinogen levels reflect the gastric functional statuses. The aim of this study is to establish appropriate serum pepsinogen cutoff points for predicting the gastric acid secretion status. In a total of 627 Japanese subjects, gastric acid secretion was measured with an endoscopic gastrin test, and the serum pepsinogen values and serum Helicobacter (H.) pylori-IgG antibody were also measured. After checking the correlation between gastric acid secretion and serum pepsinogen, the receiver operating characteristics analyses were employed for determining the most suitable cutoff points of serum pepsinogen for the gastric acid secretion status (i.e., hypochlorhydria, profound hypochlorhydria, and hyperchlorhydria). The pepsinogen I/II ratio and pepsinogen I showed the best correlation with gastric acid secretion in H. pylori-positive and H. pylori-negative subjects, respectively. The serum pepsinogen I/II ratio (or pepsinogen I in cases of H. pylori-negative subjects) was useful to determine the gastric acid secretion status with acceptable to outstanding diagnostic accuracy (the range of the area under the curve: 0.79-0.93). The diagnostic accuracy was further improved after stratifying the subjects by H. pylori-infection status. Estimating gastric acid secretion levels by simple measurement of serum pepsinogens will have significant clinical implications in estimating the risks for various diseases of the upper gastrointestinal tract.

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“…Integrated decremental response of serum PG I for 120 min after atropine injection was calculated from the area circumscribed by the curve below the basal value. Serum PG I levels were measured by radioimmunoassay described previously (Nakanome et al 1983). The assay could detect changes of 1 ng/ml with 95° confidence.…”

Section: Methodsmentioning

confidence: 99%

“…We previously investigated the serum group I pepsinogen (PG I) levels in patients with peptic ulcer and normal subjects, and demonstrated that the mean serum PG I level in the subjects roentgenologically free from gastrointestinal diseases was higher than the value in the subjects with endoscopically normal gastric and duodenal mucosa (Nakanome et al 1983). The normal range of gastric pepsin has been defined as the range in the subjects with endoscopically normal gastric mucosa (Yamagata et al 1983).…”

mentioning

confidence: 99%

“…Patients with gastric ulcer or duodenal ulcer have been found to show high levels of serum PG I (Samloff et al 1975;Nakanome et al 1983). In addition, patients wih postoperative recurrent ulcer have been reported to show a marked and significant elevation of serum PG I levels as compared with patients without postoperative recurrent ulcer (Samloff et al 1976;Stabile et al 1978).…”

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confidence: 99%

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