Serum glycated albumin as good biomarker for predicting type 2 diabetes: A retrospective cohort study of China National Diabetes and Metabolic Disorders Survey

Bai, Yuanyuan; Fang, Yujie; Ming, Jie; Wei, Huigang; Zhang, Pinghua; Yan, Juan; Du, Yanliang; Li, Qiaoyue; Yu, Xuefeng; Guo, Ming; Liang, Shengru; Hu, Ruofan; Ji, Qiuhe

doi:10.1002/dmrr.3477

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…To date, glycated human serum albumin (GHSA) has been suggested as a new promising diabetes biomarker. 10 − 14 In diabetes patients, the GHSA level can be 2–3-fold higher than that of healthy people. 15 , 16 No fasting is required for GHSA measurement.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, many HbA1c-detecting strategies have been designed for effective HbA1c detection. − However, such methods are not suitable for pregnant women and patients with kidney and/or cardiovascular diseases. , Thus, new effective biomarkers for diabetes screening and monitoring are in urgent need. To date, glycated human serum albumin (GHSA) has been suggested as a new promising diabetes biomarker. − In diabetes patients, the GHSA level can be 2–3-fold higher than that of healthy people. , No fasting is required for GHSA measurement. GHSA was reported to provide more relevant and immediate glucose information relative to HbA1c in children, adolescents, and pregnant women. , Therefore, many attempts have been made to search for GHSA detection strategies. − Previously, liquid chromatography, , affinity chromatography, , colorimetry, Raman spectroscopy, and immunochemistry were studied to detect a GHSA level; however, such methods are costly and time-consuming.…”

Section: Introductionmentioning

confidence: 99%

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Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

et al. 2023

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Diabetes mellitus is a chronic metabolic disease involving continued elevated blood glucose levels. It is a leading cause of mortality and reduced life expectancy. Glycated human serum albumin (GHSA) has been reported to be a potential diabetes biomarker. A nanomaterial-based aptasensor is one of the effective techniques to detect GHSA. Graphene quantum dots (GQDs) have been widely used in aptasensors as an aptamer fluorescence quencher due to their high biocompatibility and sensitivity. GHSA-selective fluorescent aptamers are first quenched upon binding to GQDs. The presence of albumin targets results in the release of aptamers to albumin and consequently fluorescence recovery. To date, the molecular details on how GQDs interact with GHSA-selective aptamers and albumin remain limited, especially the interactions of an aptamer-bound GQD (GQDA) with an albumin. Thus, in this work, molecular dynamics simulations were used to reveal the binding mechanism of human serum albumin (HSA) and GHSA to GQDA. The results show the rapid and spontaneous assembly of albumin and GQDA. Multiple sites of albumins can accommodate both aptamers and GQDs. This suggests that the saturation of aptamers on GQDs is required for accurate albumin detection. Guanine and thymine are keys for albumin-aptamer clustering. GHSA gets denatured more than HSA. The presence of bound GQDA on GHSA widens the entrance of drug site I, resulting in the release of open-chain glucose. The insight obtained here will serve as a base for accurate GQD-based aptasensor design and development.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

et al. 2023

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“…The excess glucose is known to bind to human serum albumin (HSA) via the nonenzymatic glycation during hyperglycemia and form glycated HSA (GHSA) . HSA is the abundant protein carrier found in blood and is commonly used as an indicator for many diseases including diabetes and renal diseases. , GHSA has been suggested to be one of the potential diabetes biomarkers since its level in blood can be used for diabetes screening and monitoring in medical practice. , GHSA detection can also be employed for patients with kidney and liver problems whose hemoglobin A1c level (which is a common diabetes biomarker) may be inaccurate.…”

Section: Introductionmentioning

confidence: 99%

Structural and Dynamic Alteration of Glycated Human Serum Albumin in Schiff Base and Amadori Adducts: A Molecular Simulation Study

et al. 2023

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Human serum albumin (HSA) is a protein carrier in blood transporting metabolites and drugs. Glycated HSA (GHSA) acts as a potential biomarker for diabetes. Thus, many attempts have been made to detect GHSA. Glycation was reported to damage the structure and ligand binding capability, where no molecular detail is available. Recently, the crystal structure of GHSA has been solved, where two glucose isomers (pyranose/GLC and open-chain/GLO) are located at Sudlow’s site I. GLO was found to covalently bind to K195, while GLC is trapped by noncontact interactions. GHSA exists in two forms (Schiff base (SCH) and Amadori (AMA) adducts), but how both disrupt albumin activity microscopically remains unknown. To this end, molecular dynamics simulations were performed here to explore the nature of SCH and AMA. Both forms are found to alter the main protein dynamics, resulting in (i) the widening of Sudlow’s site I entrance, (ii) the size reduction of nine fatty acid-binding pockets, (iii) the enlargement of Sudlow’s site I and the shrinking of Sudlow’s site II, (iv) the enhancement of C34 reactivity, and (v) the change in the W214 microenvironment. These unique characteristics found here can be useful for understanding the effect of glycation on the albumin function in more detail and designing specific and selective GHSA detection strategies.

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Section: Introductionmentioning

confidence: 99%

“…10 HbA1c alone is also a poor biomarker for prediabetes. 11 Both the HbA1c and fasting plasma glucose assays will miss patients with IGT, leading to underdiagnosis of prediabetes in high-risk populations. 12 13 OGTT is inconvenient, time-consuming, labourintensive, and relatively costly, and often perceived as unpleasant.…”

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confidence: 99%

Self-administered oral glucose tolerance test with capillary glucose measurements for the screening of diabetes mellitus in high-risk adults: a feasibility study

Tan

et al. 2021

BMJ Open Diab Res Care

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IntroductionEarly diagnosis of prediabetes based on blood sampling for the oral glucose tolerance test (OGTT) is crucial for intervention but multiple barriers hinder its uptake. This study aimed to assess the feasibility and precision of a self-administered capillary OGTT for type-2 diabetes mellitus (T2DM) in high-risk individuals.Research design and methodsParticipants with history of gestational diabetes or prediabetes were recruited in primary care. Due to their prediabetic status and previous diagnosis of gestational diabetes mellitus, a proportion of participants had previous experience doing OGTT. They self-administered the capillary OGTT and concurrently their venous glucose samples were obtained. They filled a questionnaire to collect their demographic information, views of their capillary OGTT, and their preferred site of the test.ResultsAmong 30 participants enrolled in this feasibility study, 93.3% of them felt confident of performing the capillary OGTT themselves, and 70.0% preferred the test at home. Older, less educated participants found it less acceptable. Mean capillary glucose values were significantly higher than venous glucose values, with mean difference at 0.31 mmol/L (95% CI 0.13 to 0.49) at fasting, and 0.47 mmol/L (95% CI 0.12 to 0.92) 2 hours post-OGTT. Capillary and venous glucose measurements were correlated for fasting (r=0.95; p<0.001) and 2-hour-post-OGTT (r=0.95;p<0.001). The Fleiss-Kappa Score (0.79, p<0.0001) indicated fair agreement between the two methods. The capillary OGTT had excellent sensitivity (94.1%) and negative predictive value (NPV=91.7%) in identifying prediabetes or T2DM status, vis-a-vis to venous glucose samples.ConclusionSelf-administered capillary OGTT is feasible and acceptable, especially among younger adults, with excellent sensitivity and NPV compared with plasma-based OGTT.

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Serum glycated albumin as good biomarker for predicting type 2 diabetes: A retrospective cohort study of China National Diabetes and Metabolic Disorders Survey

Cited by 9 publications

References 39 publications

Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

Structural and Dynamic Alteration of Glycated Human Serum Albumin in Schiff Base and Amadori Adducts: A Molecular Simulation Study

Self-administered oral glucose tolerance test with capillary glucose measurements for the screening of diabetes mellitus in high-risk adults: a feasibility study

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