Abstract:SummaryData are presented to show that there are three serum fl-globulin types in laboratory mice controlled by a pair of alleles. Each allele appears to give rise to three electrophoretically distinct zones in starch gel. Within the inbred strain AI AGS there was variation between mice in the intensity of staining of the three zones. Reciprocal mating data gave no evidence of an effect of fl-globulin type on segregation ratios as has been reported for cattle.
“…Buds (2 to 3 per tree) were ground in the trisHCl buffer pH 7.5 (WENDEL and WEEDEN, 1989). The enzymes were separated in 12 % starch gel, prepared in the lithium-borate electrode buffer pH 8.2, tris-citrate gel buffer pH 8.2, tris-citrate electrode buffer pH 7.0, and histidine-tris gel buffer pH 7.0 (ASHTON and BRADEN, 1961;CONKLE et al,1982;WENDEL and WEEDEN, 1989). The extracts were stored at -80°C until the electrophoretic analysis.…”
Section: Plant Materials and Isozyme Analysismentioning
Sorbus torminalis is a rare forest tree species in Poland. Allelic and genotypic structures at 25 isozyme gene loci were observed in 20 populations from Poland, situated mainly along a latitudinal gradient. Levels of genetic diversity were high both at the species level (P = 44 %, H e = 0.435) and within populations (mean P = 40.8 %, mean H e = 0.373). Levels of differentiation among populations were relatively high (F ST = 0.17) and a noticeable geographic structure of this differentiation was detected. The population in the 'Brekinia' reserve had a unique genetic structure of a clonal population and was the most distinct from the other populations.
“…Buds (2 to 3 per tree) were ground in the trisHCl buffer pH 7.5 (WENDEL and WEEDEN, 1989). The enzymes were separated in 12 % starch gel, prepared in the lithium-borate electrode buffer pH 8.2, tris-citrate gel buffer pH 8.2, tris-citrate electrode buffer pH 7.0, and histidine-tris gel buffer pH 7.0 (ASHTON and BRADEN, 1961;CONKLE et al,1982;WENDEL and WEEDEN, 1989). The extracts were stored at -80°C until the electrophoretic analysis.…”
Section: Plant Materials and Isozyme Analysismentioning
Sorbus torminalis is a rare forest tree species in Poland. Allelic and genotypic structures at 25 isozyme gene loci were observed in 20 populations from Poland, situated mainly along a latitudinal gradient. Levels of genetic diversity were high both at the species level (P = 44 %, H e = 0.435) and within populations (mean P = 40.8 %, mean H e = 0.373). Levels of differentiation among populations were relatively high (F ST = 0.17) and a noticeable geographic structure of this differentiation was detected. The population in the 'Brekinia' reserve had a unique genetic structure of a clonal population and was the most distinct from the other populations.
“…A morpholine-citrate buffer with pH 6.1 (Wendel & Weeden, 1989) was used for the following enzymes: aconitase (EC 4.2.1.3, ACO), phosphogluconate dehydrogenase (EC 1.1.1.44, PGD), phosphoglucoisomerase (EC 5.3.1.9, PGI) and shikimate dehydrogenase (EC 1.1.1.25, SKD), and a LiOH-borate buffer with pH 8.3 (Ashton & Braden, 1964) was used for aspartate aminotransferase (EC 2.6.1.1, AAT), phosphoglucoisomerase (EC 5.3.1.9, PGI) and triose phosphate isomerase (EC 5.3.1.1, TPI). Hence, both buffer systems were used for the PGI system.…”
Section: Scabiosa Columbaria L and S Canescens W And Kmentioning
We quantified allozyme diversity and quantitative genetic variation within and between six populations of the locally rare Scabiosa canescens and the more common and widespread S. columbaria (Dipsacaceae). For the within-population component, we quantified allozyme diversity as the expected heterozygosity (H e ), followed by a sib analysis to estimate the heritability (h 2 ) for eight vegetative and floral characters. There was no consistent association between h 2 and H e , or between h 2 and current population size. Regarding the betweenpopulation component, we compared the overall level of population differentiation for the allozymes (F ST ), assumed to be selectively neutral, with the corresponding estimates for the quantitative traits (Q ST ) to determine if the marker genes and the polygenic characters have been influenced by the same evolutionary force(s). Differences between estimates of F ST and Q ST for S. canescens were too small to be declared as significant, indicating that neutral phenotypic evolution has led to the observed differentiation in this species. Our data indicate higher levels of phenotypic differentiation in S. columbaria with values of Q ST exceeding the allozymic F ST for all traits. This finding indicates a great potential for S. columbaria to respond to local selection pressures, perhaps explaining why this species has a broader ecological amplitude than S. canescens.
“…1. Routine screening was performed by horisontal electrophoresis in polyacrylamide slabs using the discontinous buffer system by ASHTON and BRADEN (1961). Reference samples were provided by Dr. G .…”
Electrophoretic variants of serum transferrin were studied in a series of 4400 individuals from northern Sweden. The individuals were distributed according to place of birth into 23 subpopulations. Five different transferrin variants (B0, B0–1, B2, Dchi and DFin) were found. A significant regional variation was found for two variants (B0–1 and DChi), which show a relatively high frequency in Finland. The highest frequencies of these variants were found in parishes located along the Finnish border. Using the B0–1 and DChi variants as markers, the Finnish admixture in these parishes was estimated to about 70 per cent. The B0–1 and DChi variants appear to have been introduced into Sweden by different historical migrations.
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