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1961
DOI: 10.1071/bi9610248
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Serum ?-Globulin Polymorphism in Mice

Abstract: SummaryData are presented to show that there are three serum fl-globulin types in laboratory mice controlled by a pair of alleles. Each allele appears to give rise to three electrophoretically distinct zones in starch gel. Within the inbred strain AI AGS there was variation between mice in the intensity of staining of the three zones. Reciprocal mating data gave no evidence of an effect of fl-globulin type on segregation ratios as has been reported for cattle.

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Cited by 271 publications
(102 citation statements)
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“…Buds (2 to 3 per tree) were ground in the trisHCl buffer pH 7.5 (WENDEL and WEEDEN, 1989). The enzymes were separated in 12 % starch gel, prepared in the lithium-borate electrode buffer pH 8.2, tris-citrate gel buffer pH 8.2, tris-citrate electrode buffer pH 7.0, and histidine-tris gel buffer pH 7.0 (ASHTON and BRADEN, 1961;CONKLE et al,1982;WENDEL and WEEDEN, 1989). The extracts were stored at -80°C until the electrophoretic analysis.…”
Section: Plant Materials and Isozyme Analysismentioning
confidence: 99%
“…Buds (2 to 3 per tree) were ground in the trisHCl buffer pH 7.5 (WENDEL and WEEDEN, 1989). The enzymes were separated in 12 % starch gel, prepared in the lithium-borate electrode buffer pH 8.2, tris-citrate gel buffer pH 8.2, tris-citrate electrode buffer pH 7.0, and histidine-tris gel buffer pH 7.0 (ASHTON and BRADEN, 1961;CONKLE et al,1982;WENDEL and WEEDEN, 1989). The extracts were stored at -80°C until the electrophoretic analysis.…”
Section: Plant Materials and Isozyme Analysismentioning
confidence: 99%
“…A morpholine-citrate buffer with pH 6.1 (Wendel & Weeden, 1989) was used for the following enzymes: aconitase (EC 4.2.1.3, ACO), phosphogluconate dehydrogenase (EC 1.1.1.44, PGD), phosphoglucoisomerase (EC 5.3.1.9, PGI) and shikimate dehydrogenase (EC 1.1.1.25, SKD), and a LiOH-borate buffer with pH 8.3 (Ashton & Braden, 1964) was used for aspartate aminotransferase (EC 2.6.1.1, AAT), phosphoglucoisomerase (EC 5.3.1.9, PGI) and triose phosphate isomerase (EC 5.3.1.1, TPI). Hence, both buffer systems were used for the PGI system.…”
Section: Scabiosa Columbaria L and S Canescens W And Kmentioning
confidence: 99%
“…1. Routine screening was performed by horisontal electrophoresis in polyacrylamide slabs using the discontinous buffer system by ASHTON and BRADEN (1961). Reference samples were provided by Dr. G .…”
Section: Methodsmentioning
confidence: 99%