Serum gastrin and gastrin-like immunoreactivity of gastrointestinal mucosa in fetal, neonatal and adult rats

Kakumoto, Y; Yabana, Tsuyoshi; Sekiyama, N; Mitani, Jintai; Takasu, S.; Yachi, Akira; Wada, Takeo

doi:10.1007/bf02775449

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…Moreover, this hormone has been shown to induce or assist the maturation of struc¬ tural and functional elements in much of the gastrointestinal tract (Furihata et al 1972;Hen¬ ning & Sims 1979). A similar developmental pattern has been found in gastrin-secreting cells and serum or gastric tissue gastrin concentrations in young rats in relation to age, weaning or corticosterone admini¬ stration (Peitsch et al 1981;Lichtenberger 8c Johnson 1974; Kakumoto et al 1976;Larsson et al 1974). Accordingly, although tissue SLI con¬ tent per se cannot be used to assess the functional status of somatostatin cells, the present data sug¬ gest that feeding or corticosterone stimulates the development of somatostatin secreting cells along with gastrin secreting cells in the stomach and also that gastric somatostatin, together with gastrin, may play an important role in gastric digestion of food in young rats (Koshimizu 1983).…”

Section: Discussionsupporting

confidence: 55%

Development of gastric somatostatin-like immunoreactivity in response to corticosterone acetate and dietary changes in young rats

Okahata

Nishi

Muraki

et al. 1986

Acta Endocrinologica

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The effects of dietary changes from breast milk to solid food and corticosterone acetate administration on somatostatin-like immunoreactivity (SLI) in the gastric antrum and corpus were studied in young and adult rats. At 7 days of age, a single dose of corticosterone acetate (250 mg/kg of body weight) was injected in one group of rats, and a single dose of physiological saline was injected in another group. Each group was divided into two subgroups; one was fed only rat breast milk until 25 days of age and the other weaned (solid food alone) at 21 days of age. Gastric antrums and corpora were removed at various ages for assay of SLI concentrations by radioimmunoassay. In non-corticosterone treated rats, antral and corpus SLI increased gradually until 20 days of age, and after changing from breast milk to solid food on day 21 antral SLI increased 2-fold to the adult level, whereas corpus SLI remained constant. In corticosterone\x=req-\ treated rats, antral and corpus SLI on day 11 was 5-fold that on day 7, and thereafter remained constant before changing from breast milk to solid food; after weaning (solid food alone) antral SLI increased again to reach the adult level at 25 days of age whereas corpus SLI remained constant after weaning. Prolonged breast milk feeding alone did not influence antral or corpus SLI levels in either corticosterone-treated or non\x=req-\ treated rats as compared to levels on day 20. Gel filtration of antral and corpus SLI from 15 day old corticosterone-treated and non-treated, and 25 day old weaned (solid food alone) and unweaned (breast milk alone) rats showed almost the same results: only one peak coeluted with synthetic somatostatin-14. The resuits suggest that weaning (solid food alone) and corticosterone administration affect the antral and corpus SLI.Somatostatin is found in the antrum and corpus of the stomach and it has paracrine mechanism in gastric acid secretion (Arimura et al. 1975;Larsson 1981). It has recently been shown that steroid hormone administration in young unweaned rats results in the development of gastrointestinal en¬ zymes and hormones as seen in weaned animals (Furihata et al. 1972;Henning & Sims 1979). Especially in the stomach, the antral gastrin con¬ centration in unweaned rats increased after cor¬ ticosterone acetate administration as it did in weaned rats (Peitsch et al. 1981). Koshimizu has reported that somatostatin-like immunoreactivity (SLI) concentrations in the gastric antrum and corpus of immature rats increased with age up to 21 days of age (time of weaning) (Koshimizu 1983). In this investigastion, we studied the effects of weaning (changes from breast milk to solid food abruptly) and corticosterone acetate on an¬ tral and corpus SLI in young rats. This study differs from Koshimizu's study, particularly in its emphasis on the relationship of this peptide to dietary changes, weaning and breast milk alone until day 25 and to the effect of corticosterone administration.

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Section: Discussionsupporting

confidence: 55%

Development of gastric somatostatin-like immunoreactivity in response to corticosterone acetate and dietary changes in young rats

Okahata

Nishi

Muraki

et al. 1986

Acta Endocrinologica

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Development of antral gastrin-like immunoreactivity and pituitary CCK8/gastrin-like immunoreactivity in rats

et al. 1985

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Gel filtration of antral extract of adult rats revealed gastrin-17 and gastrin-34. Gel filtration of anterior pituitary extract showed CCK8 and gastrin-17, whereas posterior pituitary extract showed only a CCK8. Antral gastrin-like immunoreactivity (G-LI) increased after milk feeding and especially after weaning was started. Changes in diet may exert a profound influence on the ontogenic development of antral G-LI, but not pituitary CCK8/G-LI.

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Studies on immunoreactive gastrin with special regard to its molecular form and G cell counts in gastrointestinal mucosa of fetal, neonatal and adult rats

et al. 1978

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Changes of distribution of immunoreactive gastrin (IRG) and molecular forms of intra-tissue gastrin in the growth course of rats were examined. The results obtained were as follows; 1) IRG concentrations and G cell counts were extremely low in the fetal antral mucosa. However, a gradual increase of the above was observed during the neonatal suckling period, accompanied by a rapid increase at the commencement of feeding. 2) IRG concentrations in the duodenal mucosa of the fetal and neonatal period were markedly higher than that of adult rat. 3) Jejunal IRG concentration was negligible in the fetal period. The value obtained from the postnatal rats was equal to or higher than that of adult rat. 4) The major form of antral gastrin was G-17 throughout the fetal and adult age. No qualitative change of antral IRG in the growth course of rats was seen. 5) The major form of the duodeno-jejunal mucosa was G-17 in the fetal period and thereafter G-34 increased gradually in the growth course of rats. These results suggested that, (1) suckling and feeding appeared to be a trigger of the production and release of antral gastrin in the growth course of rats. (2) In the initial stage of the growth, G cells distributed in the duodeno-jejunal mucosa as well as in the antral mucosa may participated in the production and release mechanism of gastrin.

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Serum gastrin and gastrin-like immunoreactivity of gastrointestinal mucosa in fetal, neonatal and adult rats

Cited by 4 publications

References 13 publications

Development of gastric somatostatin-like immunoreactivity in response to corticosterone acetate and dietary changes in young rats

Development of gastric somatostatin-like immunoreactivity in response to corticosterone acetate and dietary changes in young rats

Development of antral gastrin-like immunoreactivity and pituitary CCK8/gastrin-like immunoreactivity in rats

Studies on immunoreactive gastrin with special regard to its molecular form and G cell counts in gastrointestinal mucosa of fetal, neonatal and adult rats

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