Serum FSH-suppressing activity of human recombinant inhibin A in male and female rats

Robertson, D. M.; Prisk, M. D.; McMaster, J. W.; Irby, D. C.; Findlay, J. K.; Kretser, David Morritz de

doi:10.1530/jrf.0.0910321

Cited by 31 publications

(10 citation statements)

References 4 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, we have found that inhibin administered intravenously exerted a marked suppression on spontaneous GH secretion in male rats, at a dose similar to that used by others to inhibit plasma FSH levels and pituitary FSH-β mRNA levels [15, 16, 17, 18]. Furthermore, in gonadectomized rats, inhibin also showed its inhibitory effect on basal GH secretion, and antagonized the stimulatory effect of DHT.…”

Section: Discussionsupporting

confidence: 69%

Inhibin Suppresses in vivo Growth Hormone Secretion

et al. 1998

View full text Add to dashboard Cite

Although the effects of inhibin on gonadotropin synthesis and secretion have been extensively studied, the role of inhibin in the neuroregulation of in vivo growth hormone (GH) secretion still remains to be elucidated. In the present work, we investigated the effects of inhibin on spontaneous GH secretion in three different groups of conscious adult male rats: intact, gonadectomized, and dihydrotestosterone (DHT)-treated gonadectomized animals. We found that inhibin administration (100 µg/kg, i.v.) led to a marked suppression in spontaneous GH secretion in all the groups studied. This significant decrease was assessed by the area under the curve in intact (311.1 ± 163.3 vs. 3,882.1 ± 1,084.6 ng/ml/6 h, p < 0.01), gonadectomized (416.6 ± 120.9 vs. 2,078.5 ± 298.4 ng/ml/6 h, p < 0.01) and gondadectomized rats treated with DHT (755.0 ± 102.3 vs. 4,539.3 ± 1,670.6 ng/ml/6 h, p < 0.01). Furthermore, intravenous inhibin significantly reduced in vivo GH responses to GHRH (10 µg/kg, i.v.) in both intact and gonadectomized rats. These findings suggest a role of inhibin on in vivo GH secretion in the male rat.

show abstract

Section: Discussionsupporting

confidence: 69%

Inhibin Suppresses in vivo Growth Hormone Secretion

et al. 1998

View full text Add to dashboard Cite

show abstract

“…The completion of a selection phase is defined in cattle by both ultrasound measurements (Savio et al, 1988;Sirois and Fortune, 1988) and the intrafollicular ratio of oestradiohprogesterone concentrations (Ireland andRoche, 1982, 1983a, b) (Richards, 1980) (Adams et al, 1992 (Price and Webb, 1988) and inhibin (Beard et al, 1990;Rivier and Vale, 1991;Robertson et al, 1991), produced by the follicle, control the release of FSH in a negative fashion. The relative importance of both hormones in the regulation of FSH concentration is not clear, but the endocrine data generated in this experiment (Fig.…”

Section: Statistical Analysesmentioning

confidence: 99%

Selection, dominance and atresia of follicles during the oestrous cycle of heifers

Sunderland

Crowe²,

Boland³

et al. 1994

Reproduction

200

150

View full text Add to dashboard Cite

show abstract

“…The preparation used (batch 7) was similar to that characterized by Tierney et al [6] and used by Robertson et al [7] to suppress serum FSH in castrated male and female rats. The preparation was stored in 35% acetonitrile containing 0.1% trifluoroacetic acid at -70 0 C. Prior to use, it was thawed and the acetonitrile was removed by evaporation under nitrogen; the remaining sample was gel filtered via a Sephadex G25 column (PD 10; Pharmacia, Uppsala, Sweden).…”

Section: Inhibinmentioning

confidence: 99%

“…This consists of a disulfide-linked heterodimer of an a and 3 A subunit with two molecular-size forms (31 kDa and 34 kDa) attributed to differing degrees of glycosylation. Robertson et al [7] demonstrated that the recombinant material could suppress serum FSH levels by 50% (with maximal suppression from 4 to 8 h postinjection) in 3-day-castrated 35-day-old male rats and by 30% (at 8 h after injection) in short-term-ovariectomized 85-day-old female rats. Serum LH was not affected by the administration of hrI in either experiment.…”

Section: Introductionmentioning

confidence: 99%

Gonadotropin-Suppressing Activity of Human Recombinant Inhibin in the Male Rat is Age Dependent1

Wreford

O’Connor

Kretser

1994

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

We have examined the age-dependent effects of human recombinant inhibin A (hrI) on gonadotropin levels in intact male rats. A single s.c. injection of hrI in the dose range 6.25-100 ng/g BW resulted in a significant and consistent decrease (20-40%) in serum FSH measured 6 h later, at the higher (50 and 100 ng/g) doses, in animals 25-91 days of age. Administration of the same doses in animals < or = 20 days old had no significant effect on serum FSH levels. Similarly, no effect of hrI (100 ng/g) on serum FSH levels was observed in 15-day-old rats when FSH levels were monitored from 1 to 8 h. No effect of hrI was observed on serum LH levels except in 40-day-old rats, which exhibited a consistent and significant reduction at the higher (25-100 ng/g) doses of inhibin. Serum inhibin levels 6 h after the injection were not significantly altered at any dose in young animals (< or = 30 days); however, in older animals (40, 50, and 91 days) there was a significant elevation in circulating inhibin levels at the higher doses, strongly suggesting an age-related change in clearance. Testicular inhibin levels were not affected by administration of the exogenous material except at the highest dose (100 ng/g) in the oldest animals (91 days), when an approximately 30% reduction in both the concentration and the total content of inhibin was observed. The data clearly demonstrate that the effect of hrI on serum gonadotropins is age dependent and that changes in clearance may contribute to the period during which circulating inhibin levels are elevated postinjection.

show abstract

Serum FSH-suppressing activity of human recombinant inhibin A in male and female rats

Cited by 31 publications

References 4 publications

Inhibin Suppresses in vivo Growth Hormone Secretion

Inhibin Suppresses in vivo Growth Hormone Secretion

Selection, dominance and atresia of follicles during the oestrous cycle of heifers

Gonadotropin-Suppressing Activity of Human Recombinant Inhibin in the Male Rat is Age Dependent1

Contact Info

Product

Resources

About