Serum from plasma rich in growth factors regenerates rabbit corneas by promoting cell proliferation, migration, differentiation, adhesion and limbal stemness

Abstract

s-PRGF promotes proliferation and migration and influences limbal stemness, adhesion and fibrosis during corneal healing.

Antioxidant Role of PRGF on RPE Cells after Blue Light Insult as a Therapy for Neurodegenerative Diseases

“…It has been widely used in oral implantology and traumatology in order to enhance wound healing [29][30][31]. With this same purpose, it has been used in ophthalmology to treat ocular surface diseases such as persistent epithelium defects (PED) and other pathologies [32][33][34][35][36]. It contributes to the acceleration of the healing process by increasing the proliferation and migration rate of cells.…”

Effects of three blood derived products on equine corneal cells, an in vitro study

“…However, there have been contradicting results in past research, some of which revealed PRGF as the most potent blood derived product [12,33]. Differences in study designs regarding use of control groups may be a contributing factor to the significant differences in results [13]. Furthermore, variable production processes for PRGF and PRP may significantly influence the outcome of in vitro studies and clinical trials [19].…”

“…A number of studies on the beneficial properties of PRP in equine orthopaedic disorders and wound healing exist [5][6][7][8]. Recently, PRP and plasma rich in growth factors (PRGF) have been introduced in human ophthalmology, with promising results [9][10][11][12][13]. PRP has proven particularly beneficial in the treatment of nonhealing corneal ulcers, otherwise resistant to medical therapy [10].…”

Levels of oxidative DNA damage are low in ex vivo engineered human limbal epithelial tissue

“…Limbal stem cell deficiency may cause recurrent corneal epithelial erosions, inflammatory reactions and ingrowth of fibro-vascular tissue onto the corneal surface. Removal of the diseased tissue and transplantation of ex vivo engineered epithelium may restore the integrity of the corneal surface (Pellegrini et al 1997;Nakamura et al 2004;Sangwan et al 2011;Pathak et al 2013;Romero et al 2014;Zakaria et al 2014;Ljubimov & Saghizadeh 2015;Szab o et al 2015;Etxebarria et al 2017;Liu et al 2017). Removal of the diseased tissue and transplantation of ex vivo engineered epithelium may restore the integrity of the corneal surface (Pellegrini et al 1997;Nakamura et al 2004;Sangwan et al 2011;Pathak et al 2013;Romero et al 2014;Zakaria et al 2014;Ljubimov & Saghizadeh 2015;Szab o et al 2015;Etxebarria et al 2017;Liu et al 2017).…”

“…Clinical symptoms include ocular pain and loss of vision (Tseng 1996;Dua et al 2000). Removal of the diseased tissue and transplantation of ex vivo engineered epithelium may restore the integrity of the corneal surface (Pellegrini et al 1997;Nakamura et al 2004;Sangwan et al 2011;Pathak et al 2013;Romero et al 2014;Zakaria et al 2014;Ljubimov & Saghizadeh 2015;Szab o et al 2015;Etxebarria et al 2017;Liu et al 2017). For such engineering, a sample containing limbal epithelium is removed from the donor eye, and cultures may be initiated by the suspension culture technique or by the explant culture technique.…”