Serum free thiols in type 2 diabetes mellitus: A prospective study

Abstract

Highlights Serum thiols are a marker of oxidative stress. High serum thiols are associated with better glycemic control and less complications in T2DM. Free thiols have limited capability for the prediction of long-term complications.

The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL

“…In addition, there was (modest) additional value for free thiols for risk prediction of long-term complications. In agreement with these and previous results, we observed in the current study that free thiols declined with ageing and were higher in men than in women [11,12], whereas we also confirmed a positive relationship with e-GFR at least in univariate regression analysis [11].…”

“…Since a negative correlation between HbA1c and thiols has been reported [12], it should be taken into account that T2DM subjects in their study were poorly regulated with an average HbA1c of 74 ± 30 mmol/mol and of 78 mmol/mol, in newly diagnosed and medically treated T2DM subjects, respectively. Recently, we measured free thiols in a population of 929 T2DM outpatients treated in primary care [12]. In this cohort, we found significant multivariable associations between free thiols and the presence of macrovascular complications, age, BMI, diastolic blood pressure, HbA1c (all inverse associations), male sex, diabetes duration and use of platelet aggregation inhibitors (all positive associations).…”

“…In a small study among 40 T2DM subjects (20 newly diagnosed and 20 treated using oral blood glucose lowering therapy only) and 20 non-diabetic subjects they demonstrated lower plasma total thiol levels among persons with T2DM as compared to control subjects (605 ± 36 versus 665 ± 60 µmol/L) [34]. Since a negative correlation between HbA1c and thiols has been reported [12], it should be taken into account that T2DM subjects in their study were poorly regulated with an average HbA1c of 74 ± 30 mmol/mol and of 78 mmol/mol, in newly diagnosed and medically treated T2DM subjects, respectively. Recently, we measured free thiols in a population of 929 T2DM outpatients treated in primary care [12].…”

“…Furthermore, among renal transplant recipients higher free thiols concentrations were associated with a beneficial cardiovascular risk profile and a better patient and graft survival [11]. Recently, we demonstrated that in T2DM patients treated in primary care with high circulating free thiols concentrations were associated with lower HbA1c levels and less microvascular complications [12].…”

Systemic Oxidative Stress Is Increased in Postmenopausal Women and Independently Associates with Homocysteine Levels

“…Mean serum free thiol concentrations were 780 ± 80.9 µM for reproductive women and 762.9 ± 85.3 µM for menopausal women (p < 0.001 after correction for age, Figure S1). Concentration ranges of serum free thiols were in accordance with previous studies that used the same detection method [20][21][22][23][24]. Median age of reproductive women was 30 years (interquartile range (IQR) 22-42 years), whereas menopausal women had a median age of 54 years (IQR: 50-59 years).…”