Serum‐free in vitro cultivation of
            <i>Theileria annulata</i>
            and
            <i>Theileria parva</i>
            schizont‐infected lymphocytes

Zweygarth, E.; Nijhof, Ard M.; Knorr, Sarah; Ahmed, Jabbar S.; AL-Hosary, Amira; Obara, Isaiah; Bishop, Richard P.; Josemans, Antoinette I.; Clausen, Peter‐Henning

doi:10.1111/tbed.13348

Cited by 10 publications

(11 citation statements)

References 18 publications

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“…In this article (Zweygarth et al, 2020), the authors would like to make the follow correction to a sentence published in section 2.1 Theileria spp. schizont‐infected cell line, 2nd paragraph on page 36, so that it reads: ‘‘All T. parva ‐infected cell lines originated from South Africa.…”

mentioning

confidence: 99%

Retracted: Corrigendum

2021

Transbound Emerg Dis

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In this article (Zweygarth et al., 2020), the authors would like to make the follow correction to a sentence published in section 2.1 Theileria spp. schizont-infected cell line, 2nd paragraph on page 36, so that it reads: ''All T. parva-infected cell lines originated from South Africa. The T. parva cell cultures 9656 and 9620 were kindly provided by Ms Boitumelo Maboko of the Onderstepoort Veterinary Institute (OVI). They were previously initiated at the OVI from the blood of calves on which ticks collected in 2017 at a cattle farm in KwaZulu-Natal province had fed.Cell culture 8160 was established in 2007 from the blood of cattle infested with Rhipicephalus zambesiensis adult ticks that had fed as nymphs on a carrier animal infected with the buffalo-derived Hluhluwe 3 isolate (Potgieter et al., 1988)."This sentence replaces the original sentence, which was: ''All T. parva-infected cell lines originated from South Africa. They were previously established at the Onderstepoort Veterinary Institute (OVI) by feeding ticks collected in a KwaZulu-Natal private game reserve in 2017 at the OVI on calves. Blood from these calves was used to initiate T. parva cell cultures 8160, 9596 and 9620.

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mentioning

confidence: 99%

Retracted: Corrigendum

2021

Transbound Emerg Dis

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“…Both Theileria species mentioned above are transmitted through tick saliva, infect lymphocytes and form macroschizonts in the vertebrate host. This causes uncontrolled cell proliferation and immortalization (Spooner et al 1989 ; Zweygarth et al 2020 ). The disease they cause has the same or similar phenotypic characteristics as cancer (Tretina et al 2015 ).…”

Section: Protozoan Diseasesmentioning

confidence: 99%

Prevention of tick-borne diseases: challenge to recent medicine

Hromníková

Furka

et al. 2022

Biologia

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Ticks represent important vectors and reservoirs of pathogens, causing a number of diseases in humans and animals, and significant damage to livestock every year. Modern research into protection against ticks and tick-borne diseases focuses mainly on the feeding stage, i.e. the period when ticks take their blood meal from their hosts during which pathogens are transmitted. Physiological functions in ticks, such as food intake, saliva production, reproduction, development, and others are under control of neuropeptides and peptide hormones which may be involved in pathogen transmission that cause Lyme borreliosis or tick-borne encephalitis. According to current knowledge, ticks are not reservoirs or vectors for the spread of COVID-19 disease. The search for new vaccination methods to protect against ticks and their transmissible pathogens is a challenge for current science in view of global changes, including the increasing migration of the human population. Highlights • Tick-borne diseases have an increasing incidence due to climate change and increased human migration • To date, there is no evidence of transmission of coronavirus COVID-19 by tick as a vector • To date, there are only a few modern, effective, and actively- used vaccines against ticks or tick-borne diseases • Neuropeptides and their receptors expressed in ticks may be potentially used for vaccine design

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“…Rhipicephalus appendiculatus ticks were collected from bovine pastures with a history of Corridor disease outbreaks in Pongola in 2017 and not from a game reserve as erroneously reported by Zweygarth et al (2020). They fed on bovines 9620 and 9656, erroneously called 9596 by Zweygarth et al (2020) at ARC-OVR quarantine facility. In vitro cultures were initiated upon presentation of T. parva clinical symptoms.…”

Section: And 9656mentioning

confidence: 99%

South African Buffalo-Derived Theileria parva Is Distinct From Other Buffalo and Cattle-Derived T. parva

et al. 2021

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Theileria parva is a protozoan parasite transmitted by the brown-eared ticks, Rhipicephalus appendiculatus and Rhipicephalus zambeziensis. Buffaloes are the parasite’s ancestral host, with cattle being the most recent host. The parasite has two transmission modes namely, cattle–cattle and buffalo–cattle transmission. Cattle–cattle T. parva transmission causes East Coast fever (ECF) and January disease syndromes. Buffalo to cattle transmission causes Corridor disease. Knowledge on the genetic diversity of South African T. parva populations will assist in determining its origin, evolution and identify any cattle–cattle transmitted strains. To achieve this, genomic DNA of blood and in vitro culture material infected with South African isolates (8160, 8301, 8200, 9620, 9656, 9679, Johnston, KNP2, HL3, KNP102, 9574, and 9581) were extracted and paired-end whole genome sequencing using Illumina HiSeq 2500 was performed. East and southern African sample data (Chitongo Z2, Katete B2, Kiambu Z464/C12, Mandali Z22H10, Entebbe, Nyakizu, Katumba, Buffalo LAWR, and Buffalo Z5E5) was also added for comparative purposes. Data was analyzed using BWA and SAMtools variant calling with the T. parva Muguga genome sequence used as a reference. Buffalo-derived strains had higher genetic diversity, with twice the number of variants compared to cattle-derived strains, confirming that buffaloes are ancestral reservoir hosts of T. parva. Host specific SNPs, however, could not be identified among the selected 74 gene sequences. Phylogenetically, strains tended to cluster by host with South African buffalo-derived strains clustering with buffalo-derived strains. Among the buffalo-derived strains, South African strains were genetically divergent from other buffalo-derived strains indicating possible geographic sub-structuring. Geographic sub- structuring was also observed within South Africa strains. The knowledge generated from this study indicates that to date, ECF is not circulating in buffalo from South Africa. It also shows that T. parva has historically been present in buffalo from South Africa before the introduction of ECF and was not introduced into buffalo during the ECF epidemic.

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Serum‐free in vitro cultivation of Theileria annulata and Theileria parva schizont‐infected lymphocytes

Cited by 10 publications

References 18 publications

Retracted: Corrigendum

Retracted: Corrigendum

Prevention of tick-borne diseases: challenge to recent medicine

South African Buffalo-Derived Theileria parva Is Distinct From Other Buffalo and Cattle-Derived T. parva

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