Serum ferritin concentration and isoferritin patterns detected by immunoradiometric assay.

Abstract

SummaryTwo-site immunoradiometric assay for serum ferritin was developed by anti-human liver ferritin antiserum applied on polyvinyl V bottom microtiter plates. The coefficient of variation was 4.4-5.9% of liver ferritin dissolved in 1/4 diluted human serum at a concentration of 0.625-125ng/ml. The lowest concentration (0.625ng/ml) was statistically distinguishable from the background by Student's t test (p<0.05). Isoferritin patterns of serum ferritin and tissue ferritins were examined by the combination of gel isoelectric focussing and 2-site immunoradiometric assay in order to explore any similarities in pIs within these ferritins. Although serum ferritin had wide range of pIs, its basic isomers corresponded well to those of liver and spleen ferritin. Geometric means and their 95% confidence limits in serum ferritin concentration were 70 ng/ml and 12-411ng/ml, respectively, in sera of 205 healthy males (16-25 years), and 12ng/ml and 1-211ng/ml, respectively, in 421 healthy females (16-59 years). Keywords serum ferritin, isoferritin, anti-human liver ferritin, immuno radiometric assay, gel isoelectric focussing, p1, liver ferritin Ferritin is a high molecular weight iron-containing protein, which is distributed intracellularly in many tissues and can frequently be found by conventional methods in the sera of patients with certain liver diseases (1). However, recent progress in immunoradiometric assay disclosed it as a common component of all sera (2-6) and showed its parallel relation to the amount of storage iron (1,4,(7)(8)(9). Accordingly, serum ferritin concentration has drawn attention as being a reliable indicator in evaluating iron nutrition. The present study describes a modification of 2-site immunoradiometric assay for ferritin using polyvinyl V-bottom microtiter plates (10) coated by anti-human liver ferritin antiserum as an immunoadsorbent, and discusses the rationality of the antiserum 87

Improvement in Iron Deficiency Anemia through Therapy with Ferric Ammonium Citrate and Vitamin C and the Effects of Aerobic Exercise.

“…Iron deficiency anemia is a common nutritional problem in premenopausal women in Japan (1,2) and most other countries, affecting more than 10% of the world population (3)(4)(5)(6)(7). Various iron preparations have been used for the treat ment of iron deficiency anemia (8)(9)(10)(11).…”

Determination of Absorption and Endogenous Excretion of Iron in Man by Monitoring Fecal Excretion of a Stable Iron Isotope (58Fe).

“…The iron absorption calculated by our formula showed good agreement with that estimated by Janghorbani's formula. binding capacity (TIBC) were immunochemically analyzed (16). The measure ment of the dietary intake of iron from the foods ingested by volunteers was carried out at Japan Food Research Laboratory (Osaka, Japan).…”