Serum Factor in Renal Compensatory Hyperplasia

Lowenstein, Leah M.; Stern, Arnold

doi:10.1126/science.142.3598.1479

Cited by 97 publications

(36 citation statements)

References 5 publications

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“…Many diverse type experiments and assays suggest the presence of a circulating renotropic factor [8,11,[13][14][15][16][17][18]20]. Some investigators have injected test sera into living animals and estimated DNA synthesis by the incorporation of :IH-Tdr into DNA [10,19] or added test sera to tissue cultures and measured the mitotic index [13]. In 1970, we found that sera removed from UNI rats enhance :1H-Tdr monophosphate incorporation into DNA [18], Over the following years, using a similar in vitro assay system, we described a renotropic system in rats [16].…”

Section: Discussionmentioning

confidence: 99%

Autoradiographic Studies of the Rat Renotropic System

Castillo¹,

Robertson²,

Goldin³

et al. 1980

Nephron

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Rat sera, 10–30 h after unilateral nephrectomy (UNI), enhance ³H-thymidine (³H-Tdr) incorporation into DNA of incubating renal tissue from control rats. Stimulation is even greater when extracts from remaining growing kidneys 20 h after UNI are combined with sera from rats after UNI. UNI extracts, i. e., extracts from the kidney remaining after uninephrectomy, are nonstimulatory alone. UNI sera and UNI sera plus UNI extracts could theoretically augment ³H-Tdr incorporation into renal DNA via dilutional means rather than enhanced DNA synthesis. To determine if our results were secondary to enhanced DNA synthesis, we performed our in vitro assay using the labelling of nuclei via autoradiography as another index. The addition of UNI sera compared to sera from sham-operated rats (SHAM) in seven paired experiments enhanced incorporation of ³H-Tdr into DNA by 30% (p < 0.02) and the addition of both UNI sera and UNI extracts compared to SHAM sera and SHAM extracts enhanced incorporation by 48% (p < 0.001). Unlike a dilutional effect, nuclear labelling also increased in these same seven experiments: UNI sera versus SHAM sera increased 25% (p < 0.05) and UNI sera + UNI extracts versus SHAM sera + SHAM extracts increased 37% (p < 0.01). We conclude that UNI sera and UNI sera + UNI extracts enhance ³H-Tdr incorporation into DNA by augmenting DNA synthesis, driving cells into the ‘S’ phase. The use of ³H-Tdr incorporation into DNA in our assay does estimate DNA synthesis.

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Section: Discussionmentioning

confidence: 99%

Autoradiographic Studies of the Rat Renotropic System

Castillo¹,

Robertson²,

Goldin³

et al. 1980

Nephron

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“…DISCUSSION Existence of circulating renotropins, factors affecting some aspect of renal cellular growth, is supported by the ability of sera from UNI animals to enhance isotopic labeling of nucleic acids in renal tissue cultures and intact kidneys (10)(11)(12)(13)(14)(15)(16). In 1970, we utilized an in vitro assay to detect a circulating renotropin that stimulates DNA synthesis as measured by isotopic labeling of DNA in rat kidney slices (3).…”

mentioning

confidence: 99%

A renotropic system in rats.

Preuss¹,

Goldin²

1976

J. Clin. Invest.

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“…There have been numerous reports of circulating or humoral factors which alter renal RNA and/or DNA metabolism [6,11,12,17,20], Factors that both stimulate [11,12] and inhibit [20] biochemical growth have been described. Increased RNA and/or DNA accumulation would, thus, result from in creased levels of renal responsiveness to a stimulating factor or from decreased levels of renal responsiveness to an inhibiting fac tor.…”

Section: Discussionmentioning

confidence: 99%

“…and (2) What is the relationship between the biochemical (RNA and DNA) and the physiological (RBF and GFR) changes? While numerous experimentals manipulations and plasma or urine factors have been shown to alter renal RNA, DNA and/or protein metabolism [6,11,12,17,20], the absence of information on the effects of these on renal function precludes definitive conclusions being made with re gard to the overall 'growth' response. Thus resolution of the second question is requisite to elucidation of possible mechanisms.…”

Section: Introductionmentioning

confidence: 99%

RNA and DNA Contents of Kidneys from Rats with Unilateral Ureteral Ligation

Murray¹,

Malvin²,

Northrup³

1979

Kidney Blood Press Res

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Renal RNA and DNA contents, as well as estimates of renal plasma flow and glomerular filtration rate, were studied in albino Sprague-Dawley rats subjected to either unilateral nephrectomy and/or unilateral ureteral ligation. RNA and DNA contents of ligated kidneys were markedly higher than those of the contralateral, unligated kidney 24 and 48 h post ligation. These increases in RNA and DNA content of the ligated kidney occurred in the presence of relative decreases inreal plasma flow and glomerular filtration rate in the ligated kidney. Renal RNA and DNA contents were also increased in the remaining kidney following unilateral nephrectomy. The effect of ureteral ligation and unilateral nephrectomy on renal RNA content was found to be additive. These results are inconsistent with theories stating that changes in renal RNA and DNA content are the result of increased renal plasma flow and glomerular filtration rate. The data support a role for a circulating factor which either stimulates renal RNA and DNA accumulation from the lumental aspect of tubular cells or inhibits renal RNA and DNA accumulation from the peritubular aspect of these cells.

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Serum Factor in Renal Compensatory Hyperplasia

Cited by 97 publications

References 5 publications

Autoradiographic Studies of the Rat Renotropic System

Autoradiographic Studies of the Rat Renotropic System

A renotropic system in rats.

RNA and DNA Contents of Kidneys from Rats with Unilateral Ureteral Ligation

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