Serum exosomal miRNA as biomarkers for Retinoblastoma

Ravishankar, Hemanth; Mangani, Abubakar Siddiq; Moses, Grace Lydia Phoebe; Mani, Sathya; Parameswaran, Sowmya; Khetan, Vikas; Ganesan, Suganeswari; Krishnakumar, Subramanian

doi:10.1016/j.exer.2020.108184

Cited by 12 publications

(10 citation statements)

References 23 publications

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“…In this comprehensive study, we characterized RB serum sEVs coding and non-coding RNA content, and explored the miRNA-mRNA, and lncRNA-miRNA-mRNA regulatory interactions. Until now, only one previous study had analyzed small non-coding miRNAs in serum sEVs of RB patients using NGS technology [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, RB sEV miRNA expression profiles form our study correlated well with previous RB serum vs. control serum miRNA profiles by Beta et al [ 62 ] ( Table S9 ). Previous RB sEVs miRNA data revealed that except 301b-3p and 216b-5p, the miRNA expression in sEVs and corresponding RB tumor tissue samples were not matching [ 21 ]. Interestingly, these two miRNAs were not expressed in our samples.…”

Section: Discussionmentioning

confidence: 99%

“…In pursuit of identifying EV-based biomarkers, various clinical trials have been completed for prostate cancer (NCT02702856, NCT03031418, NCT03235687, NCT04720599), and a few are in progress for lung (NCT05058768), oropharyngeal (NCT02147418), and ovarian cancers (NCT03738319) [ 13 , 14 , 15 , 16 ]. However, studies on RB EVs are limited and are confined to RB cell line, and primary cell cultures established from enucleated eyes of RB patients [ 17 , 18 , 19 , 20 , 21 ]. sEVs derived from WERI RB1 cells were shown to promote tumor growth and mediate tumor deterioration in RB murine xenograft model via miR-92a, 20a, 129a, and 17, C-X-C chemokine receptor type 4, and thrombospondin-1 [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, RB-derived small EVs can also promote the angiogenesis of human vesicle endothelial cells via miR-92a-3p [ 19 ]. Moreover, only the shortlisted sEV miRNAs from RB cell lines have been validated in RB tumors and corresponding serum sEVs [ 21 ]. Nonetheless, the comprehensive analysis of total RNA contents (coding and non-coding (ncRNAs)) and their interactions in RB serum sEVs has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Analysis of Serum Small Extracellular Vesicles-Derived Coding and Non-Coding RNAs from Retinoblastoma Patients for Identifying Regulatory Interactions

Manukonda

Yenuganti

Nagar

et al. 2022

Cancers

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The present study employed nanoparticle tracking analysis, transmission electron microscopy, immunoblotting, RNA sequencing, and quantitative real-time PCR validation to characterize serum-derived small extracellular vesicles (sEVs) from RB patients and age-matched controls. Bioinformatics methods were used to analyze functions, and regulatory interactions between coding and non-coding (nc) sEVs RNAs. The results revealed that the isolated sEVs are round-shaped with a size < 150 nm, 5.3 × 1011 ± 8.1 particles/mL, and zeta potential of 11.1 to −15.8 mV, and expressed exosome markers CD9, CD81, and TSG101. A total of 6514 differentially expressed (DE) mRNAs, 123 DE miRNAs, and 3634 DE lncRNAs were detected. Both miRNA-mRNA and lncRNA-miRNA-mRNA network analysis revealed that the cell cycle-specific genes including CDKNI1A, CCND1, c-MYC, and HIF1A are regulated by hub ncRNAs MALAT1, AFAP1-AS1, miR145, 101, and 16-5p. Protein-protein interaction network analysis showed that eye-related DE mRNAs are involved in rod cell differentiation, cone cell development, and retinol metabolism. In conclusion, our study provides a comprehensive overview of the RB sEV RNAs and regulatory interactions between them.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comprehensive Analysis of Serum Small Extracellular Vesicles-Derived Coding and Non-Coding RNAs from Retinoblastoma Patients for Identifying Regulatory Interactions

Manukonda

Yenuganti

Nagar

et al. 2022

Cancers

View full text Add to dashboard Cite

show abstract

“…The presence of exosomal miRNAs from RB primary tissue samples was analyzed in the corresponding serum samples by Ravishankar et al But, none of the tested miRNAs which are expressed in RB cell lines and primary RB tissue samples were expressed in serum. Probably, serum exosomal miRNAs are not ideal prognostic factors for RB, rather exosomal miRNAs derived from other ocular fluids such as AH and VH may serve the purpose [114]. Various clinical trials have been initiated for investigating exosomes as diagnostic and prognostic biomarkers for non-ocular cancers.…”

Section: Exosomes In Rbmentioning

confidence: 99%

Exosomes in the visual system: New avenues in ocular diseases

Manukonda

Attem

Yenuganti

et al. 2022

TUB

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Exosomes are a subgroup of membrane-bound extracellular vesicles secreted by all cell types and present virtually in all biological fluids. The composition of exosomes in the same cell type varies in healthy and disease conditions. Hence, exosomes research is a prime focus area for clinical research in cancer and numerous age-related metabolic syndromes. Functions of exosomes include crucial cell-to-cell communication that mediates complex cellular processes, such as antigen presentation, stem cell differentiation, and angiogenesis. However, very few studies reported the presence and role of exosomes in normal physiological and pathological conditions of specialized ocular tissues of the eye and ocular cancers. The eye being a protected sense organ with unique connectivity with the rest of the body through the blood and natural passages, we believe that the role of exosomes in ocular tissues will significantly improve our understanding of ocular diseases and their interactions with the rest of the body. We present a review that highlights the existence and function of exosomes in various ocular tissues, their role in the progression of some of the neoplastic and non-neoplastic conditions of the eyes.

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Tumor‐associated exosomes in cancer progression and therapeutic targets

Liu,

Wu,

Pan

et al. 2024

MedComm

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Exosomes are small membrane vesicles that are released by cells into the extracellular environment. Tumor‐associated exosomes (TAEs) are extracellular vesicles that play a significant role in cancer progression by mediating intercellular communication and contributing to various hallmarks of cancer. These vesicles carry a cargo of proteins, lipids, nucleic acids, and other biomolecules that can be transferred to recipient cells, modifying their behavior and promoting tumor growth, angiogenesis, immune modulation, and drug resistance. Several potential therapeutic targets within the TAEs cargo have been identified, including oncogenic proteins, miRNAs, tumor‐associated antigens, immune checkpoint proteins, drug resistance proteins, and tissue factor. In this review, we will systematically summarize the biogenesis, composition, and function of TAEs in cancer progression and highlight potential therapeutic targets. Considering the complexity of exosome‐mediated signaling and the pleiotropic effects of exosome cargoes has challenge in developing effective therapeutic strategies. Further research is needed to fully understand the role of TAEs in cancer and to develop effective therapies that target them. In particular, the development of strategies to block TAEs release, target TAEs cargo, inhibit TAEs uptake, and modulate TAEs content could provide novel approaches to cancer treatment.

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Serum exosomal miRNA as biomarkers for Retinoblastoma

Cited by 12 publications

References 23 publications

Comprehensive Analysis of Serum Small Extracellular Vesicles-Derived Coding and Non-Coding RNAs from Retinoblastoma Patients for Identifying Regulatory Interactions

Comprehensive Analysis of Serum Small Extracellular Vesicles-Derived Coding and Non-Coding RNAs from Retinoblastoma Patients for Identifying Regulatory Interactions

Exosomes in the visual system: New avenues in ocular diseases

Tumor‐associated exosomes in cancer progression and therapeutic targets

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