Serum deprivation alters lipid profile in HN9.10e embryonic hippocampal cells

Garcia‐Gil, Mercedes; Lazzarini, Andrea; Lazzarini, Remo; Floridi, Emanuela; Cataldi, Samuela; Floridi, Ardesio; Albi, Elisabetta

doi:10.1016/j.neulet.2014.12.059

Cited by 9 publications

(10 citation statements)

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“…(2011) by using the Ultra Performance Liquid Chromatography system tandem mass spectrometer (Applied Biosystems, Italy). The lipid species were separated, identified, and analyzed as previously reported ( Garcia-Gil et al. , 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Very-long-chain fatty acid sphingomyelin in nuclear lipid microdomains of hepatocytes and hepatoma cells: can the exchange from C24:0 to C16:0 affect signal proteins and vitamin D receptor?

Lazzarini

Macchiarulo

Floridi

et al. 2015

MBoC

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Section: Methodsmentioning

confidence: 99%

Very-long-chain fatty acid sphingomyelin in nuclear lipid microdomains of hepatocytes and hepatoma cells: can the exchange from C24:0 to C16:0 affect signal proteins and vitamin D receptor?

Lazzarini

Macchiarulo

Floridi

et al. 2015

MBoC

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“…Analyses were carried out as reported by as reported by Lazzarini et al [ 42 ] using the Ultra Performance Liquid Chromatography system tandem mass spectrometer (Applied Biosystems, Italy). The lipid species were separated, identified, and analyzed as previously reported [ 42 , 43 ]. The samples were separated on a Phenomenex Kinetex phenyl-hexyl 100 A column (50 × 4.60-mm diameter, 2.6-μm particle diameter) with a precolumn security guard Phenomenex ULTRA phenyl-hexyl 4.6.…”

Section: Methodsmentioning

confidence: 99%

“…The system needed to be reconditioned for 5 min with 50 % solvent B before the next injection. The SM species were identified by using positive turbo-ion spray and modality multipole-reaction monitoring as reported by Lazzarini et al [ 42 , 43 ].…”

Section: Methodsmentioning

confidence: 99%

Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health

et al. 2016

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BackgroundSphingomyelin plays very important roles in cell function under physiological and pathological conditions. Physical and chemical stimuli produce reactive oxygen species that stimulate acid sphingomyelinase to induce apoptosis. Antioxidant plants of the traditional Chinese Pharmacopoeia, such as Lycium Barbarum and Lycium Chinense, have become increasingly popular in Western countries. We investigated the effects of Lycium Chinense on acid sphingomyelinase and sphingomyelin species in relation to gene expression.MethodsWe prepared Lycium Chinense berry extracts and evaluated their antioxidant properties. Increasing amount of extracts was used to test cytotoxic and genotoxic effect on HepG2 cells. Gene expression, protein amount and enzyme activity of acid sphingomyelinase were tested by RT-PCR, immunoblotting and enzymatic activity assay, respectively. Sphingomyelin species were analyzed by UFLC MS/MS. A panel of 96 genes involved in oxidative stress, proliferation, apoptosis and cancer was used to test the effect of LC on gene expression. GLRX2, RNF7, and PTGS1 proteins were analyzed by immunoblotting.ResultsWe showed that Lycium Chinense berries have high antioxidant properties, have an IC50value of 9.55 mg/mL, do not induce genotoxic effect and maintain high level of cell viability. The berry extracts inhibit acid sphingomyelinase activity and increase both very long fatty acid sphingomyelin species and unsaturated fatty acid sphingomyelin species. Among 96 genes, Lycium Chinense berries up-regulate Glutaredoxin 2 and Ring Finger Protein 7 genes and proteins, able to protect cells from apoptosis. Intrigantly, Lycium Chinense berries down-regulates Prostaglandin H synthase 1 gene but the protein is not expressed in HepG2 cells.ConclusionThe results identify acid sphingomyelinase as a novel target of Lycium Chinense berries to decrease saturated/unsaturated fatty acid sphingomyelin ratio, known to be useful for cell health. Consistent with these data, the berries regulate specifically gene expression to protect cells from apoptosis.

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“…However, CERS1 elimination decreases ganglioside levels, and this might be one of the causes of neural cell death in mice (Ginkel et al, ). Moreover, serum deprivation‐induced apoptosis in embryonic hippocampal cells induces an increase in C16:0‐ceramide and a decrease in C24:0‐ceramide (Garcia‐Gil et al, ). It is worth noting that compensatory mechanisms can occur following gene knockout (KO) or when the protein expression of a substance is reduced by administration of its siRNA.…”

Section: Roles Of Bioactive Sls In the Nervous Systemmentioning

confidence: 99%

Crosstalk between sphingolipids and vitamin D3: potential role in the nervous system

Garcia‐Gil

Pierucci

Vestri

et al. 2017

British J Pharmacology

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Sphingolipids are both structural and bioactive compounds. In particular, ceramide and sphingosine 1-phosphate regulate cell fate, inflammation and excitability. 1-α,25-dihydroxyvitamin D3 (1,25(OH) 2 D 3 ) is known to play an important physiological role in growth and differentiation in a variety of cell types, including neural cells, through genomic actions mediated by its specific receptor, and non-genomic effects that result in the activation of specific signalling pathways. 1,25(OH) 2 D 3 and sphingolipids, in particular sphingosine 1-phosphate, share many common effectors, including calcium regulation, growth factors and inflammatory cytokines, but it is still not known whether they can act synergistically. Alterations in the signalling and concentrations of sphingolipids and 1,25(OH) 2 D 3 have been found in neurodegenerative diseases and fingolimod, a structural analogue of sphingosine, has been approved for the treatment of multiple sclerosis. This review, after a brief description of the role of sphingolipids and 1,25(OH) 2 D 3 , will focus on the potential crosstalk between sphingolipids and 1,25(OH) 2 D 3 in neural cells.Abbreviations 1,25(OH) 2 D 3 , 1-α,25-dihydroxyvitamin D3; AD, Alzheimer disease; APP, amyloid precursor protein; Aβ, amyloid β peptide;These Tables list key protein targets and ligands in this article which are hyperlinked to corresponding entries in http://www.guidetopharmacology.org, the common portal for data from the IUPHAR/BPS Guide to PHARMACOLOGY (Southan et al., 2016), and are permanently archived in the Concise Guide to PHARMACOLOGY 2015/16 ( a,b,c,d,e,f Alexander et al., 2015a.

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Serum deprivation alters lipid profile in HN9.10e embryonic hippocampal cells

Cited by 9 publications

References 28 publications

Very-long-chain fatty acid sphingomyelin in nuclear lipid microdomains of hepatocytes and hepatoma cells: can the exchange from C24:0 to C16:0 affect signal proteins and vitamin D receptor?

Very-long-chain fatty acid sphingomyelin in nuclear lipid microdomains of hepatocytes and hepatoma cells: can the exchange from C24:0 to C16:0 affect signal proteins and vitamin D receptor?

Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health

Crosstalk between sphingolipids and vitamin D3: potential role in the nervous system

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