Serum depletion of holo-ceruloplasmin induced by silver ions in vivo reduces uptake of cisplatin

Copper Import into the Mitochondrial Matrix in Saccharomyces cerevisiae Is Mediated by Pic2, a Mitochondrial Carrier Family Protein

“…However, we sought to find a competitor that could more directly affect mitochondrial copper. Silver shares similar electronic properties with copper, and it is often used as a toxic mimetic of copper in biological systems (28,29)…”

Non-hepatic tumors change the activity of genes encoding copper trafficking proteins in the liver

“…Holo-Cp accounts for ~60% of the serum copper in mice. 44,45 Therefore, increased serum copper concentrations are likely to be caused by an increase in the Cp pool alone. Cp activity and copper concentrations also increased in Min mice ( Fig.…”

“…The deficiency of holo-Cp developed completely by the seventh day on the Ag diet, the levels and activity of intracellular cuproenzymes did not change. 44 In Ag mice, the level of Cp protein did not decrease as determined by rocket immunoelectrophoresis. In WB assay in non-denaturing PAGE Cp of Ag mice is revealed as two bands with different mobility, both of which correspond to the 130 kDa protein.…”

“…In WB assay in non-denaturing PAGE Cp of Ag mice is revealed as two bands with different mobility, both of which correspond to the 130 kDa protein. 44,48 Ag mice were utilized to assess the influence of circulatory copper levels on tumor growth. The effect of the Cp copper deficit on tumor growth was tested in two models: the HCT116 xenograft model and the B16 melanoma model.…”

The Extracellular Domain of Human High Affinity Copper Transporter (hNdCTR1), Synthesized by<em> E. coli</em> Cells, Chelates Silver and Copper Ions <em>in Vivo</em>

“…The synthesized peptide was conjugated with hemocyanin isolated from red king crab (Paralithodes camtschaticus), and the complex was used for immunization of the rabbits. To test the specificity of the obtained antibodies, conjugates of p15 with succinylated bovine serum albumin (BSA) were applied [41]. The antibodies to P15/hemocyanin interacted with modified BSA/P15 and P15 displaced them from the immunocomplex; also, they detected the polypeptide corresponding to mono-and trimer of CTR1 on the plasma membrane of murine hepatocytes.…”