Serum Cytokine of Il-2, Il-10 and Il-12 Levels in Patients With Stomach Adenocarcinoma

Shokrzadeh, Mohammad; Mohammadpour, Abbas; Hoseini, Vahid; Abediankenari, Saeid; Ghassemi-Barghi, Nasrin; Tabari, Yahya Saleh

doi:10.1590/s0004-2803.201800000-83

Cited by 17 publications

(18 citation statements)

References 42 publications

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“…Controversial results have been reported where IL-4 and IL-10 either support or hinder tumor progression [63, 64]. In fact, it has been indicated that a lack of Il-10 allows induction of pro-inflammatory cytokines hampering anti-tumor immunity [65], and that an increased level might be used as diagnostic biomarker in certain cancer such as stomach adenocarcinoma [66]. Tanikawa et al; have shown that a lack of IL-10 promotes tumor development, growth and metastases [63].…”

Section: Discussionmentioning

confidence: 99%

Anti-proliferative effects of mesenchymal stem cells (MSCs) derived from multiple sources on ovarian cancer cell lines: an in-vitro experimental study

et al. 2019

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Mesenchymal stem cells (MSCs) have surfaced as ideal candidates for treatment of different therapeutically challenging diseases however their effect on cancer cells is not well determined. In this study, we investigated the effect of MSCs derived from human bone marrow (BM), adipose tissue (AT), and umbilical cord derived MSCs (UC-MSCs) on ovarian cancer. Measurements of ovarian tumor marker proteins were computed by ELISA. Proliferative, apoptosis and anti-inflammatory effects of the MSCs were measured by Flow cytometry (FCM). MMPs expression was measured by RT-PCR. The co-culture of cancer cell lines OVCAR3, CAOV3, IGROV3 and SKOV3 with the conditioned media of MSCs (CM-MSC) and MSCs showed an increase in cellular apoptosis, along with a reduction in the level of CA-125 and a decline of LDH and beta-hCG. A decrease in CD24 of the cancer cell lines in co-culture with the CM-MSCs showed a reduction of the cancer tumorigenicity. In addition, the invasion and aggressiveness of cancer cell lines was significantly decreased by CM-MSC; this was translated by a decrease in MMP-2, MMP-9, and CA-125 mRNA expression, and an increase in TIMP 1, 2, and 3 mRNA expression. An increase in IL-4 and IL-10 cytokines, and a decrease in GM-CSF, IL-6, and IL-9, were also noted. In conclusion, mesenchymal stem cells derived from different sources and their conditioned media appear to have a major role in inhibition of cancer aggressiveness and might be considered as a potential therapeutic tool in ovarian cancer.

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Section: Discussionmentioning

confidence: 99%

Anti-proliferative effects of mesenchymal stem cells (MSCs) derived from multiple sources on ovarian cancer cell lines: an in-vitro experimental study

et al. 2019

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“…Patients IL-10 levels Healthy donor References Melanoma 15-480 pg/ml <3.0 pg/ml [35] 8.75 pg/ml <3.0 pg/ml [39] 24.3 ng/ml 3.4 ng/ml [41] Gastric cancer 6.3 ng/ml 3.4 ng/ml [41] 12.5 pg/ml 4.0 pg/ml [40] 27.52 pg/ml <12 np/ml [51] 19.6 pg/ml 9.2 pg/ml [52] >21.0 pg/ml <3.0 pg/ml [53] Pancreatic cancer 6.8 ng/ml 3.4 ng/ml [41] >10.0 pg/ml ND [54] >9 clinical outcome imparted by IL10 genetic variants may be mediated by effects on the tumor micro-environment composition or modulation [47]. The possibility that SNPs participate in the development, establishment, and the prognosis of cancer has been investigated by numerous research groups and includes several group malignances.…”

Section: Diseasementioning

confidence: 99%

IL10 as Cancer Biomarker

González‐Garza¹,

Cruz-Vega²,

Maldonado‐Bernal³

2021

Translational Research in Cancer

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Chronic inflammation can trigger events that would induce the malignant transformation of cells and carcinogenesis. Cytokines play a crucial role and can control the development and multiplication of cancerous cells. However, clinical data present controversy about the participation of these proteins in the establishment and development of cancer. Interleukin 10 (IL-10), a potent anti-inflammatory cytokine, has been the subject of multiple studies. Several studies have reported that IL-10 has pro-and antitumor effects. Elevated levels of IL-10 are associated with increased tumor growth with poor prognosis and drug resistance. However, this cytokine has both tumor-promoting and tumor-inhibiting properties. In vitro and in vivo studies report mechanisms by which IL-10 expression downregulates class I, which results in the control of the metastatic disease. IL-10 also inhibits tumorigenesis via downregulation of other cytokines. The variation observed could be the result of concentration ranges of this protein, genetic polymorphism, or both. The value obtained may serve as a biomarker indicative of tumor development and its prognosis.

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“…This assay was shown to have a very wide applicability for measuring survival and/ or proliferation of various cells and can potentially be applied to any assay in which living cells must be distinguished from dead cells or a lack of cells. Besides, it showed the potential value for quantitative and rapid measurement of cell death (7)(8)(9) . Moreover, we used micronucleus assay to assess genetic damage of aripiprazole in human peripheral blood lymphocytes.…”

Section: Cytotoxic Effects Of Aripiprazole On Mkn45 and Nih3t3 Cell Lmentioning

confidence: 99%

Cytotoxic Effects of Aripiprazole on Mkn45 and Nih3t3 Cell Lines and Genotoxic Effects on Human Peripheral Blood Lymphocytes

Shokrzadeh

Mohammadpour

Modanloo

et al. 2019

Arq. Gastroenterol.

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BACKGROUND: Gastric cancer is known as the fourth most common cancer. Current treatments for cancer have damaged the sensitive tissues of the healthy body, and in many cases, cancer will be recurrent. Therefore, need for treatments that are more effective is well felt. Researchers have recently shifted their attention towards antipsychotic dopamine antagonists to treat cancer. The anticancer activities of aripiprazole remain unknown. OBJECTIVE: This study aimed to evaluate the efficacy and safety of aripiprazole on gastric cancer and normal cell lines. METHODS: In this regard, the cytotoxicity and genotoxicity of aripiprazole were investigated in MKN45 and NIH3T3 cell lines by methyl tetrazolium assay and on peripheral blood lymphocytes by micronucleus assay. For this purpose, cells were cultured in 96 wells plate. Stock solutions of aripiprazole and cisplatin were prepared. After cell incubation with different concentrations of aripiprazole (1, 10, 25, 50, 100 and 200 μL), methyl tetrazolium solution was added. For micronucleus assay fresh blood was added to RPMI culture medium 1640 supplemented, and different concentrations of aripiprazole (50, 100 and 200 μL) were added. RESULTS: The finding of present study showed that the IC50 of aripiprazole in the cancer cell line (21.36 μg/mL) was lower than that in the normal cell line (54.17 μg/mL). Moreover, the micronucleus assay showed that the frequency of micronuclei of aripiprazole at concentrations below 200 μM was much less than cisplatin. CONCLUSION: Aripiprazole can be a good cytotoxic compound and good candidate for further studies of cancer therapy.

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Serum Cytokine of Il-2, Il-10 and Il-12 Levels in Patients With Stomach Adenocarcinoma

Cited by 17 publications

References 42 publications

Anti-proliferative effects of mesenchymal stem cells (MSCs) derived from multiple sources on ovarian cancer cell lines: an in-vitro experimental study

Anti-proliferative effects of mesenchymal stem cells (MSCs) derived from multiple sources on ovarian cancer cell lines: an in-vitro experimental study

IL10 as Cancer Biomarker

Cytotoxic Effects of Aripiprazole on Mkn45 and Nih3t3 Cell Lines and Genotoxic Effects on Human Peripheral Blood Lymphocytes

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