Serum creatine kinase B subunit levels in neurogenic atrophies

Jockers-Wretou, Evangelia; Vassilopoulos, D.

doi:10.1007/bf00313786

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…Scrum CK-B activity was determined by a new solid-phase direct enzyme immunoassay (EIA). de tails of which have already been published [10]. Briefly, anti-CK-BB-coated plastic cuvettes were in cubated with the patients' sera.…”

Section: Isoenzyme Assaysmentioning

confidence: 99%

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Serum Creatine Kinase B Levels in Diseases of the Central Nervous System

Vassilopoulos¹,

Jockers-Wretou²

1987

Eur Neurol

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The creatine kinase B (CK-B) subunit was determined by our own highly sensitive solid-phase direct immunoassay in three neurological diseases which represent models of central nervous system (CNS) involvement: (1) idiopathic epilepsy (18 patients), a disease characterised by a transient neuronal hyperactivity; (2) Parkinson’s disease (17 patients), a degenerative disorder of the CNS, and (3) multiple sclerosis (21 patients), which represents a model of demyelination of the CNS. A group of 50 controls was also studied. The results showed that in patients with epilepsy and multiple sclerosis the CK-B activity was considerably lower than in the controls (p < 0.01 and p < 0.005, respectively), while no differences were found between the controls and the patients with Parkinson’s disease. It is suggested that the low CK-B activity observed might be due to the medication taken or to the disease process itself. The present findings have to be considered as an indication for further CK isoenzyme studies in systematic disorders of the nervous system.

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Section: Isoenzyme Assaysmentioning

confidence: 99%

“…Briefly, anti-CK-BB-coated plastic cuvettes were in cubated with the patients' sera. The CK-B activity measured was referred to a standard calibration curved with purified CK-MB or CK-BB isoenzyme [9,10,II]. The CK-B measurements were made blindly, i.e.…”

Section: Isoenzyme Assaysmentioning

confidence: 99%

Serum Creatine Kinase B Levels in Diseases of the Central Nervous System

Vassilopoulos¹,

Jockers-Wretou²

1987

Eur Neurol

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“…We have recently shown that cTnT, arguably reflecting neuromuscular involvement, but not cTnI, is commonly increased in the serum of patients with ALS, correlating with clinical severity as measured using the revised ALS Functional Rating Scale (ALSFRS-R) and thus could be of utility to distinguish the disease from ALS mimics or other neurodegenerative diseases [9]. While a few previous case reports have also reported elevations in CK-MB and CK-B subunit in the sera of patients with neuromuscular diseases including ALS [10,11], again presumably reflecting neuromuscular involvement, a role of CK-MB as a biomarker for ALS has never been formally explored.…”

Section: Introductionmentioning

confidence: 99%

Creatine Kinase MB Isoenzyme Is a Complementary Biomarker in Amyotrophic Lateral Sclerosis

Kittipeerapat,

Fabian,

Bernsen

et al. 2023

IJMS

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Amyotrophic lateral sclerosis (ALS) is an invariably fatal neurodegenerative disease with limited therapeutic options. There is an urgent need for novel biomarkers to be used as surrogates for new therapeutic trials and disease monitoring. In this study, we sought to systematically study creatine kinase isoenzyme MB (CK-MB) in a real-world cohort of ALS patients, assess the diagnostic performance, and evaluate its association with other laboratory and clinical parameters. We reviewed data from 194 consecutive patients that included 130 ALS patients and 64 disease control patients (primary lateral sclerosis [PLS], benign fasciculations syndrome [BFS], Huntington’s disease [HD] and Alzheimer’s disease [AD]). CK-MB was elevated in the sera of more than half of all patients with ALS. In patients with spinal-onset ALS, CK-MB levels were significantly higher than in patients with other neurodegenerative diseases. Patients with slower rates of functional decline had a significantly higher baseline CK-MB. Furthermore, CK-MB elevations correlated with cardiac troponin T (cTnT) and with revised ALS Functional Rating Scale (ALSFRS-R) bulbar subcategory. We posit that measuring CK-MB in ALS patients in a complimentary fashion could potentially aid in the diagnostic workup of ALS and help discriminate the disease from some ALS mimics and other neurodegenerative diseases. CK-MB levels also may provide valuable prognostic information regarding disease aggressiveness as well as correlations with specific phenotypic presentations.

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“…Immunological investigations are increasingly used to understand the role of histones in chromatin structures and functions. Western blotting is a powerful tool to study histone topography [17], histone gene expression [18], or postsynthetic modifications of histones during different nuclear processes [19]. Towbin et al [20] have described a procedure for the transfer of proteins from SDS-PAGE, or gels containing only urea to nitrocellulose membranes.…”

Section: Introductionmentioning

confidence: 99%

Rapid and effective Western blotting of histones from acid‐urea‐Triton and sodium docecyl sulfate polyacrylamide gels: Two different approaches depending on the subsequent qualitative or quantitative analysis

Thiriet

Albert

1995

Electrophoresis

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An improved method for the electrophoretic transfer of histones from sodium dodecyl sulfate (SDS) and acetic acid-urea-Triton X-100 (AUT) polyacrylamide gels onto nitrocellulose membranes is described. In the case of SDS-gels, it was not essential to equilibrate them before transfer while for the AUT-gels, an equilibration step is essential to prevent the interference of Triton X-100 with the binding of histones to nitrocellulose. Transfer efficiency was different for different histone classes. Two procedures were developed: (1) one suitable for qualitative studies, and (ii) another for quantitative transfer.

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Serum creatine kinase B subunit levels in neurogenic atrophies

Cited by 5 publications

References 16 publications

Serum Creatine Kinase B Levels in Diseases of the Central Nervous System

Serum Creatine Kinase B Levels in Diseases of the Central Nervous System

Creatine Kinase MB Isoenzyme Is a Complementary Biomarker in Amyotrophic Lateral Sclerosis

Rapid and effective Western blotting of histones from acid‐urea‐Triton and sodium docecyl sulfate polyacrylamide gels: Two different approaches depending on the subsequent qualitative or quantitative analysis

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