Serum constituents analyses in dairy cows: Effects of duration and temperature of the storage of clotted blood

Ehsani, A.; Afshari, Asma; Bahadori, H.; Mohri, Mehrdad; Seifi, Hesam A

doi:10.1016/j.rvsc.2008.02.004

Cited by 18 publications

(23 citation statements)

References 4 publications

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“…Quanto às análises séricas para o fósforo, não houve diferença significativa entre os tempos de armazenamento avaliados, o mesmo verificado por Ehsani et al (2008) …”

Section: Resultsunclassified

“…O tempo de armazenamento pode variar de dias a meses ou mesmo anos, e a temperatura também pode oscilar durante o período de armazenamento; isso ocorre por que muitas vezes torna-se inviável realizar as análises logo após a colheita, especialmente em animais de produção. Na medicina veterinária até agora, pouco tem sido publicado sobre a estabilidade do sangue total e de muitos marcadores bioquímicos (DEVANAPALLI et al, 2002;EHSANI, et al, 2008 Russel e Roussel (2007), a estabilidade dos analitos varia, mas a maioria é estável em temperatura de refrigeração (4 ºC) por 24 a 36 horas, e, se um prazo mais longo for necessário antes que a amostra possa ser processada, o soro pode ser congelado a -20 ºC, pois o congelamento tem míninos efeitos sobre a maioria dos analitos. Entretanto, vários congelamentos e descongelamentos de uma amostra devem ser evitados, pois isso afeta a estabilidade de várias substâncias, especialmente as enzimas.…”

Section: Introductionunclassified

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Efeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicos

et al. 2011

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“…Quanto às análises séricas para o fósforo, não houve diferença significativa entre os tempos de armazenamento avaliados, o mesmo verificado por Ehsani et al (2008) …”

Section: Resultsunclassified

Section: Introductionunclassified

Efeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicos

et al. 2011

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“…In such cases, degradation of labile analytes must be prevented by refrigerating or freezing the samples. A lot of authors stated that not only physiological conditions and factors (e.g., age, sex, pregnancy, and nutritional status) but also inadequate biological sample storage, as a potential source of preanalytical errors, may markedly affect the concentrations of many biochemical variables [5–7]. However, the influence of sample storage on the concentrations of less frequently measured biochemical variables, including acute phase proteins, and their stability during storage in veterinary medicine is less well documented.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform

Tóthová

Nagy

Seidel

et al. 2012

Veterinary Medicine International

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The objective of this study was to evaluate the effect of storage under various conditions on the concentrations of major bovine acute phase protein—serum amyloid A, and its mammary isoform. Blood samples were taken from seven clinically healthy calves, and milk samples from six clinically healthy dairy cows. The harvested blood serum and the milk samples were fractioned into aliquots. One aliquot was analyzed on the day of collection without storage. The second aliquots were stored at 4°C for 1 day, the remaining aliquots were kept frozen at −18°C for 2, 7, 14, and 21 days, and then analyzed. Blood serum was analyzed for serum amyloid A (SAA). The concentrations of mammary isoform of SAA (M-SAA) were measured in milk samples. Over time, the concentrations of SAA in serum showed a tendency of significant decrease during storage at −18°C (P<0.01). Similarly, the values of M-SAA decreased significantly in samples maintained at freezer temperatures (P<0.001). In the refrigerated samples, we found non-significantly lower values of SAA, as well as M-SAA. Presented results indicate that the storage temperature and duration may markedly affect the concentrations of bovine SAA and M-SAA.

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“…Hemolysis had no effect on any of these parameters in bovine blood (Jacobs et al, 1992). Many biochemical tests are unaffected by hemolysis, or the effects are species-specific (Wesson et al, 1979;Ehsani et al, 2008). Since hemolysis is usually preventable, it would be prudent for individuals who attempt to collect blood from free-ranging wildlife to be aware of the causes of hemolysis, ways to prevent hemolysis, and effects of hemolysis on interpretation of serum biochemical results.…”

Section: Discussionmentioning

confidence: 99%

Serum Biochemistry and Serum Cortisol Levels of Immobilized and Hunted Muskoxen (<i>Ovibos moschatus</i>) from Northern Canada

Harms¹,

Elkin²,

Gunn³

et al. 2012

ARCTIC

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Muskoxen (Ovibos moschatus) are Arctic-adapted ruminants native to the Arctic regions of Canada, Alaska, and Greenland. Little is known about the serum biochemistry and serum cortisol of this species, or the effects of chemical immobilization on serum biochemical parameters. This study aimed to describe blood chemistry parameters and cortisol levels in hunted, tame, and chemically immobilized muskoxen and to examine differences in blood chemistry parameters and levels of stress associated with different capture techniques. Serum was collected from 91 adult female muskoxen in northern Canada. For analysis, these muskoxen were classified into six groups, five of free-ranging muskoxen (10 animals shot from snowmobiles near Kugluktuk, Nunavut; 18 chemically immobilized from a helicopter near Kugluktuk; 8 chemically immobilized from a helicopter near Norman Wells, Northwest Territories; 17 shot from snowmobiles near Cambridge Bay, Nunavut; 33 chemically immobilized from a snowmobile near Kugluktuk) and one of tame muskoxen (five tame animals maintained on pasture near Saskatoon, Saskatchewan). All samples were analyzed for cortisol, and 26 serum biochemistry parameters were measured in serum collected from three of the six groups (n = 36). Comparison of four groups showed that serum cortisol levels of muskoxen chemically immobilized from a helicopter near Kugluktuk were significantly higher (p < 0.05) than those of muskoxen chemically immobilized from snowmobiles or shot. A comparison of serum biochemistry from the groups of muskoxen shot and immobilized near Kugluktuk found that serum sodium, creatinine, phosphorus, magnesium, and creatine kinase were significantly higher (p < 0.05) in hunted muskoxen than in chemically immobilized animals, while urea, glucose and gamma glutamyl transferase were significantly higher (p < 0.05) in immobilized muskoxen. Most serum biochemical parameters, however, were similar to those of captive muskoxen. This evidence of differences between hunted and immobilized muskoxen in several serum biochemistry parameters will contribute to further research on the effects of immobilization and other health assessments in this species.

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Serum constituents analyses in dairy cows: Effects of duration and temperature of the storage of clotted blood

Cited by 18 publications

References 4 publications

Efeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicos

Efeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicos

The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform

Serum Biochemistry and Serum Cortisol Levels of Immobilized and Hunted Muskoxen (<i>Ovibos moschatus</i>) from Northern Canada

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