Serum Calprotectin (S100A8/A9): A Promising Biomarker in Diagnosis and Follow-up in Different Subgroups of Juvenile Idiopathic Arthritis

La, Céline; Lê, Phu Quoc; Ferster, Alina; Goffin, Laurence; Spruyt, Delphine; Lauwerys, Bernard; Durez, Patrick; Boulanger, C.; Соколова, Татяна; Rasschaert, Joanne; Badot, Valérie

doi:10.21203/rs.3.rs-138436/v2

Cited by 3 publications

(4 citation statements)

References 30 publications

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“…In a recent study by Darwish et al [41]They observed that MSUS is extremely sensitive for early diagnosis of joint involvement in JIA when compared to physical examination, since MSUS identified more synovitis than clinical examination (subclinical synovitis). n agreement with C line a et al [35] active illness was associated with serum calprotectin levels that were more than twice as high as those seen in individuals whose disease was in remission (6555ng/mL vs. 11403ng/mL), demonstrating a strong correlation between disease activity and calprotectin levels.…”

Section: Discussionsupporting

confidence: 90%

“…Methotrexate continues to be the most extensively used traditional DMARD for the treatment of JIA due to its efficacy in disease control and tolerable toxicity. n agreement with aC line et al [35] and Frosch et al [6]who revealed that the blood level of calprotectin in JIA patients was significantly higher than in the control group. These findings are consistent with the physiological explanation that calprotectin is an acute phase reactant that is typically expressed by monocytes, granulocytes, and macrophages in an early differentiation stage, following their activation by damage-associated molecular patterns (DAMPs) as a result of inflammation [36,37].CLP is accountable for leukocyte motility and trafficking, as well as inflammation amplification.…”

Section: Discussionsupporting

confidence: 89%

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Serum Level of Calprotectin in Juvenile Idiopathic Arthritis and Its Correlation with Disease Activity Clinicaland Ultrasonographic

Elfeky

Mohamed²,

Hussein³

et al. 2022

JAMMR

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Background: Calprotectin is an essential proinflammatory component of the innate immune system. Musculoskeletal ultrasound (MSUS) was shown to be a relevant and accurate evaluation method for people with chronic inflammatory arthropathies. Therefore, it has been deemed promising for assessing the joints of children with Juvenile Idiopathic Arthritis (JIA). This research aims to determine the blood level of calprotectin in patients with juvenile idiopathic arthritis (JIA) and its connection with activity measures and ultrasonographically identified synovitis. Methods: This study was carried out on 30 JIA patients, and 30 apparentlyhealthy children as controlsof matching age and sex. All patients were subjected to the following: history taking, complete clinical examination,disease activity assessment, full laboratory investigations, Ultrasound examination and enzyme-linked immunosorbent assay (ELISA) to assay the level of calprotectin in serum samples. Results: Oligoarticular was the most common type of JIA.Methotrexate was the most common DMARD used either alone or with systemic corticosteroids.There was significant difference between JIA patients and controls as regard haemoglobin, ESRand CRP. JIA patients had significantly higher serumcalprotectin levels when compared to controls.Systemic subtype was founded to have higher serum calprotectin levels. There was significant positive correlation between serum calprotectin and clinical parameters of disease activity (JADAS 27), CRP, ESR, serum ferritin and ultrasound score (EULAR-OMERACT combined scoring system for grading synovitis).There was insignificant correlation between serum calprotectin levels and RF, ANA and AntiCCP in patients’ group.Therewas positive significant correlation between ultrasound score (EULAR-OMERACT combined scoring system for grading synovitis) and disease activity (JADAS 27). Conclusion: Our research demonstrates a substantial correlation between serum calprotectin levels and clinical, laboratory, and ultrasound measures of joint inflammation. Serum In ordinary clinical practise, calprotectin is a helpful biomarker that, together with other indicators such as CRP and ESR and our clinical judgement, helps us make treatment choices. Indeed, this protein is very stable and readily detectable in serum.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

Serum Level of Calprotectin in Juvenile Idiopathic Arthritis and Its Correlation with Disease Activity Clinicaland Ultrasonographic

Elfeky

Mohamed²,

Hussein³

et al. 2022

JAMMR

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“…This highlights the utility of P-MRP8/14 as a tool for disease activity in patients with newly-onset JIA. Moreover, in a recent study, La et al found that S-MRP8/14 has more specificity than CRP does as a diagnostic tool and marker of disease activity for JIA [25].…”

Section: Discussionmentioning

confidence: 99%

Myeloid-related protein 8/14 in plasma and serum in patients with new-onset juvenile idiopathic arthritis in real-world setting in a single center

et al. 2022

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Objective The aim of this study was to analyze the usefulness of myeloid-related protein 8/14 (MRP8/14) in the prediction of disease course in a real-world setting for patients with new-onset juvenile idiopathic arthritis (JIA), to identify the relationship between MRP8/14 and disease activity using the physician’s global assessment of disease activity (PGA), and determine whether the MRP8/14 levels measured in serum and plasma are equally useful. Methods In this prospective follow-up study, 87 new-onset non-systemic JIA patients were studied. Blood and synovial fluid samples were collected prior to any antirheumatic medication use. MRP8/14 was measured from serum (S-MRP8/14), plasma (P-MRP8/14), and synovial fluid samples using ELISA. Results The baseline MRP8/14 blood levels were significantly higher in patients using synthetic antirheumatic drugs than in patients with no systemic medications at 1 year after diagnosis in serum (mean 298 vs. 198 ng/ml, P < 0.001) and in plasma (mean 291 vs. 137 ng/ml, P = 0.001). MRP8/14 levels at the time of JIA diagnosis were higher in patients who started methotrexate during 1.5-year follow-up compared to those who achieved long-lasting inactive disease status without systemic medications (serum: mean 298 vs. 219 ng/ml, P = 0.006 and plasma: 296 vs. 141 ng/ml, P = 0.001). P-MRP8/14 was the most effective predictive variable for disease activity (by PGA) in linear multivariate regression model (combined to ESR, CRP, leukocytes, and neutrophils), whereas S-MRP8/14 was not significant. Conclusion Blood MRP8/14 levels at baseline seem to predict disease course in new-onset JIA patients. P-MRP8/14 might be better than S-MRP8/14 when assessing disease activity at the time of JIA diagnosis.

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“…S100 proteins may activate innate immune cells, predominantly monocytes and macrophages, upon binding the RAGE receptor or TLR4 by enhancing the secretion of proinflammatory molecules (187). High concentrations of S100 proteins (both S100A8/A9 and S100A12) are measured in patients with sJIA and therefore these molecules were proposed to be biomarkers (188)(189)(190)(191)(192)(193)(194)(195). The release of IL-1 and other inflammatory cytokines by total white blood cells (WBCs) or monocytes from sJIA patients was reduced upon depleting serum S100A8/A9 or by preventing the S100A12 complex formation (191,196,197).…”

Section: How Neutrophilia May Fuel the Pathogenesis Of Sjiamentioning

confidence: 99%

Neutrophil Homeostasis and Emergency Granulopoiesis: The Example of Systemic Juvenile Idiopathic Arthritis

et al. 2021

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Neutrophils are key pathogen exterminators of the innate immune system endowed with oxidative and non-oxidative defense mechanisms. More recently, a more complex role for neutrophils as decision shaping cells that instruct other leukocytes to fine-tune innate and adaptive immune responses has come into view. Under homeostatic conditions, neutrophils are short-lived cells that are continuously released from the bone marrow. Their development starts with undifferentiated hematopoietic stem cells that pass through different immature subtypes to eventually become fully equipped, mature neutrophils capable of launching fast and robust immune responses. During severe (systemic) inflammation, there is an increased need for neutrophils. The hematopoietic system rapidly adapts to this increased demand by switching from steady-state blood cell production to emergency granulopoiesis. During emergency granulopoiesis, the de novo production of neutrophils by the bone marrow and at extramedullary sites is augmented, while additional mature neutrophils are rapidly released from the marginated pools. Although neutrophils are indispensable for host protection against microorganisms, excessive activation causes tissue damage in neutrophil-rich diseases. Therefore, tight regulation of neutrophil homeostasis is imperative. In this review, we discuss the kinetics of neutrophil ontogenesis in homeostatic conditions and during emergency myelopoiesis and provide an overview of the different molecular players involved in this regulation. We substantiate this review with the example of an autoinflammatory disease, i.e. systemic juvenile idiopathic arthritis.

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Serum Calprotectin (S100A8/A9): A Promising Biomarker in Diagnosis and Follow-up in Different Subgroups of Juvenile Idiopathic Arthritis

Cited by 3 publications

References 30 publications

Serum Level of Calprotectin in Juvenile Idiopathic Arthritis and Its Correlation with Disease Activity Clinicaland Ultrasonographic

Serum Level of Calprotectin in Juvenile Idiopathic Arthritis and Its Correlation with Disease Activity Clinicaland Ultrasonographic

Myeloid-related protein 8/14 in plasma and serum in patients with new-onset juvenile idiopathic arthritis in real-world setting in a single center

Neutrophil Homeostasis and Emergency Granulopoiesis: The Example of Systemic Juvenile Idiopathic Arthritis

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