Serum biomarkers of hepatitis B virus infected liver inflammation: A proteomic study

Abstract

Hepatitis B virus (HBV), a serious infectious and widespread human pathogen, represents a major health problem worldwide. Chronic HBV infection has a very high risk of evolving into hepatocellular carcinoma. Although considerable progress was made during the recent past, the pathogenesis of HBV infection is still elusive and a definite diagnosis of HBV infected liver information still relies on biopsy histological test. In this report, we used proteomics technology to globally examine HBV infected serum samples aiming at searching for disease-associated proteins that can be used as serological biomarkers for diagnosis and/or target proteins for pathogenetic study. By comparing with normal and HBV negative serum samples, we found that at least seven proteins were significantly changed in HBV infected sera. These greatly altered proteins were identified to be haptoglobin beta and alpha2 chain, apolipoprotein A-I and A-IV, alpha1-antitrypsin, transthyretin and DNA topoisomerase IIbeta. The alteration of these proteins is displayed not only in quantity but also in patterns (or specificity), which can be correlated with necroinflammatory scores. In particular, apolipoprotein A-I presents heterogeneous change in expression level with different isoforms and alpha1-antitrypsin produces evidently different fragments implying diverse cleavage pathways. These unique phenomena appear specific to HBV infection. A combination simultaneously considering the quantities and isoforms of these proteins could be a useful serum biomarker (or index) for HBV diagnosis and therapy.

Progress and Challenges in Screening for Early Detection of Ovarian Cancer

“…Of the 66 cases of nonmalignant disease, 63 were recognized as not cancer. This result yielded a sensitivity of 100%, specificity of 95% (87)(88)(89)(90)(91)(92)(93)(94)(95)(96)(97)(98)(99), and PPV of 94% (101). Although the limitations of this study design and its analysis have been discussed in some detail in the literature (103)(104)(105)(106), the potential implications of such proteomic spectrum analysis for the identification of novel tumor markers is huge.…”

Effects of cadmium on structure and enzymatic activity of Cu,Zn‐SOD and oxidative status in neural cells

“…Triplicate electrophoreses were performed to ensure reproducibility. All gels were visualized by silver staining [He et al, 2003]. Image acquisition and analysis were performed with an Image Scanner (Amersham) and Image Master 2D Elite software (Amersham).…”

“…These protein spots were cut off in small pieces and subjected to in-gel trypsin digestion overnight. Peptide mass spectra were recorded and previously described parameters for spectral acquisition were used [He et al, 2003]. Protein identification with 25 ppm or lower mass error and MOWSE scores over 300 were obtained in most analyses during database matching using MS-Fit (http://prospector.ucsf.…”

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Diagnostic value of serum haptoglobin protein as hepatocellular carcinoma candidate marker complementary to α fetoprotein

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