Serum biomarkers associated with SARS-CoV-2 severity

Batista, Fabiani de Morais; Puga, Marco Antonio Moreira; Silva, Patrícia Vieira da; Oliveira, Roberto Dias de; Santos, Paulo César Pereira dos; Silva, Bruna Oliveira da; Tatara, Mariana Bento; Tsuha, Daniel Henrique; Pires, Maria Aparecida dos Santos; Gonçalves, Crhistinne Cavalheiro Maymone; Silva, Rômulo Pessoa e; Ferreira, Nathália Tavares; Albuquerque, Amanda Vasconcelos de; Duarte, Giselle; Consolaro, Márcia Edilaine Lopes; Negrão, Fábio Juliano; Ferrari, Idalina Cristina; Cavalcanti, Luciano Pamplona de Góes; Trinta, Karen Soares; Ribeiro, Guilherme S.; Rêgo, Moacyr Jesus Barreto de Melo; Boyton, Rosemary J.; Siqueira, André; Altmann, Daniel M.; Croda, Júlio

doi:10.1038/s41598-022-20062-5

Cited by 16 publications

(17 citation statements)

References 59 publications

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“…The level of cytokines and immunologic dysregulation play a role in the severity of COVID-19 as well as multiple organ failure, respiratory distress syndrome, and septic shock [ 57 , 58 ]. Hospitalized COVID-19 patients with ICU admission have higher plasma levels of IL-2, IL-7, IL-10, G-CSF, IP10, MCP-1, MIP-1A, and TNF compared to non-ICU patients [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, levels of IL-2, IL-2R, IL-4, IL-8, IFN- γ , and specifically IL-6 and IL-10 were significantly increased in severe compared to nonsevere cases [ 18 , 51 ]. IL-6 is the primary trigger of CRS inducing the production of CRP by the liver; importantly, previous data showed a significant increase in IL-6 and CRP in severely ill patients [ 24 , 37 , 51 ] and thus predicted fatal outcomes [ 57 ]. In this study, we did not detect a significant difference in IL-6 levels (pro-inflammatory cytokine) between ICU and non-ICU admitted patients.…”

Section: Discussionmentioning

confidence: 99%

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Clinical Characteristics and Serum Cytokines Profiling in Hospitalized COVID-19 Patients in Lebanon

Hassan

Hamdan

Melhem

2023

Journal of Immunology Research

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Since its emergence, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains a public health threat worldwide. While the majority of patients recover in 3–4 weeks, complications in severely ill patients, including acute respiratory distress syndrome, cardiac injury, thrombosis, and sepsis, can lead to death. Several biomarkers, in addition to cytokine release syndrome (CRS), have been associated with severe and fatal outcomes in coronavirus disease 2019 (COVID-19) patients. The aim of this study is to assess clinical characteristics and cytokines profiles in hospitalized COVID-19 patients in Lebanon. A total of 51 hospitalized COVID-19 patients were recruited between February 2021 and May 2022. Clinical data and sera were collected at two time points: at hospital presentation (T0) and last collected results during hospitalization (T1). Our results showed that 49% of participants were >60 years with males accounting for the majority (72.5%). Hypertension, followed by diabetes and dyslipidemia, were the most frequent comorbid conditions among study participants accounting for 56.9% and 31.4%, respectively. Chronic obstructive pulmonary disease (COPD) was the only significantly different comorbid condition between intensive care unit (ICU) and non-ICU patients. Our results also showed that the median level of D-dimer was significantly elevated among patients in ICU and those who died compared to non-ICU patients and those who survived. Moreover, C-reactive protein (CRP) levels were significantly higher at T0 compared to T1 in ICU and non-ICU patients. The median level of IL-12p70 was significantly higher in patients >60 years compared to those ≤60 years ( p = 0.0209 ). Our data are in agreement with previous reports suggesting the importance of IL-6, CRP, and IL-12p70 in the assessment of risk of severe disease and mortality.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Clinical Characteristics and Serum Cytokines Profiling in Hospitalized COVID-19 Patients in Lebanon

Hassan

Hamdan

Melhem

2023

Journal of Immunology Research

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“…At the same time, in Deer-RECs, there is also an upregulation of VEGFA (0.60 log 2 FC) and HBEGF (0.34 log 2 FC) (Supplementary Data), which are associated with enhancing compensatory lung growth through paracrine signaling 62 . However, in human cases, the upregulation of serum biomarkers VEGFA and HBEGF was associated with the severity of SARS-CoV-2 infection 63 .…”

Section: Transcriptional Activation Of the Nf-κb Signaling Pathway Is...mentioning

confidence: 92%

How Do Deer Respiratory Epithelial Cells Weather The Initial Storm of SARS-CoV-2?

Davila

Nelli

Phadke

et al. 2023

Preprint

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The potential infectivity of SARS-CoV-2 in animals raises a public health and economic concern, particularly the high susceptibility of white-tailed deer (WTD) to SARS-CoV-2. The disparity in the disease outcome between humans and WTD is very intriguing, as the latter are often asymptomatic, subclinical carriers of SARS-CoV-2. To date, no studies have evaluated the innate immune factors responsible for the contrasting SARS-CoV-2-associated disease outcomes in these mammalian species. A comparative transcriptomic analysis in primary respiratory epithelial cells of human (HRECs) and WTD (Deer-RECs) infected with SARS-CoV-2 was assessed throughout 48 hours post inoculation (hpi). Both HRECs and Deer-RECs were susceptible to SARS-COV-2, with significantly (P < 0.001) lower virus replication in Deer-RECs. The number of differentially expressed genes (DEG) gradually increased in Deer-RECs but decreased in HRECs throughout the infection. The ingenuity pathway analysis of DEGs further identified that genes commonly altered during SARS-CoV-2 infection mainly belong to cytokine and chemokine response pathways mediated via IL-17 and NF-κB signaling pathways. Inhibition of the NF-κB signaling in the Deer-RECs pathway was predicted as early as 6 hpi. The findings from this study could explain the lack of clinical signs reported in WTD in response to SARS-CoV-2 infection as opposed to the severe clinical outcomes reported in humans.

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“…However, challenges and limitations exist, such as the absence of a consensus on universally predictive biomarkers across diverse populations and setting. (3). Therefore, understanding the disease's pathogenesis and finding appropriate biomarkers to know the severity of the patient's condition is crucial in mitigating its harmful health consequences.…”

Section: Introductionmentioning

confidence: 99%

Dysregulated Leukotriene Metabolism in Patients with COVID-19

Doğan,

Budak,

Doğan

et al. 2024

Jpn J Infect Dis

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The aim of this study was to examine leukotriene metabolism in COVID-19. A total of 180 people were included in the study. Of these, 60 were healthy controls, 60 were patients who needed intensive care unit (ICU), and 60 were patients who did not need intensive care (non-ICU). Serum levels of 5-lipoxygenase (5-LO), 5-LO activating protein (ALOX5AP) and cysteinyl leukotriene (CYSLT) were measured and mRNA expressions of 5-LO, ALOX5AP and cysteinyl leukotriene receptor 1 (CYSLTR1) were investigated. As compared to the control group, both non-ICU and ICU groups had lower levels of 5-LO and mRNA expression.ICU patients had lower levels of 5-LO and mRNA expression compared with non-ICU patients. The expression of CYSLTR1 mRNA was higher in patients compared to healthy controls. CYSLTR1 mRNA expression was found to be higher in ICU group than in non-ICU group. CYSLT levels were higher in the control group compared to both non-ICU and ICU patients. Due to the higher expressions of CYSLTR1 in patients than control group, selective leukotriene receptor blockers can be used as a treatment option. CYSLTR1 expressions were also higher in ICU group than non-ICU group. Thus, CYSLTR1 mRNA expression could be a promising biomarker of COVID-19 severity.

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Serum biomarkers associated with SARS-CoV-2 severity

Cited by 16 publications

References 59 publications

Clinical Characteristics and Serum Cytokines Profiling in Hospitalized COVID-19 Patients in Lebanon

Clinical Characteristics and Serum Cytokines Profiling in Hospitalized COVID-19 Patients in Lebanon

How Do Deer Respiratory Epithelial Cells Weather The Initial Storm of SARS-CoV-2?

Dysregulated Leukotriene Metabolism in Patients with COVID-19

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