Serum biomarker levels for musculoskeletal disease in two‐ and three‐year‐old racing Thoroughbred horses: A prospective study of 130 horses

Frisbie, David D.; Ilwraith, C. W. Mc; Arthur, Rick M.; Blea, J.; Baker, V. A.; Billinghurst, R. Clark

doi:10.1111/j.2042-3306.2010.00123.x

Cited by 38 publications

(58 citation statements)

References 35 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MMP‐3 was selected as it degrades other minor proteins in tendon matrix, including collagens III, IV, IX, and X, and also proteoglycans and elastin 28, 29, 30, 31, 32, 33. The C1,2C (or COL 2 3/4C short) antibody detects the carboxy terminus of fragmented type I and II collagen, cleaved by collagenases MMP‐1, MMP‐8 or MMP‐13 21, 34, 35, 36, 37…”

Section: Methodsmentioning

confidence: 99%

Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise

Spiesz

Thorpe

Chaudhry

et al. 2015

Journal Orthopaedic Research

View full text Add to dashboard Cite

The role of inflammation in tendon injury is uncertain and a topic of current interest. In vitro studies of tendon accelerated overload damage can serve as a valuable source of information on the early stages of tendinopathy. Viable fascicle bundles from bovine flexor tendons were subjected to cyclic uniaxial loading from 1–10% strain. Immuno‐staining for inflammatory markers and matrix degradation markers was performed on the samples after mechanical testing. Loaded samples exhibited visible extracellular matrix damage, with disrupted collagen fibers and fiber kinks, and notable damage to the interfascicular matrix. Inflammatory markers COX‐2 and IL‐6 were only expressed in the cyclically loaded samples. Collagen degradation markers MMP‐1 and C1,2C were colocalized in many areas, with staining occurring in the interfascicular matrix or the fascicular tenocytes. These markers were present in control samples, but staining became increasingly intense with loading. Little MMP‐3 or MMP‐13 was evident in control sections. In loaded samples, some sections showed intense staining of these markers, again localized to interfascicular regions. This study suggests that inflammatory markers may be expressed rapidly after tendon overload exercise. Interestingly, both inflammation and damage‐induced matrix remodeling seem to be concentrated in, or in the vicinity of, the highly cellular interfascicular matrix. © 2015 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc. J Orthop Res 33:889–897, 2015.

show abstract

Section: Methodsmentioning

confidence: 99%

Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise

Spiesz

Thorpe

Chaudhry

et al. 2015

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…Current research into the identification of serum biomarkers suggests that these may become valuable indicators of pathology in the future and allow for detection of subclinical disease. [57][58][59] In RER, suggestive clinical signs are associated with significantly elevated serum creatinine kinase (CK) enzyme activity 4 to 6 hours postexercise, indicating cell membrane damage because of myonecrosis. 56 Serum muscle enzyme activity does not always reflect the severity of clinical signs, but serial measurements can be used to determine the progression of injury.…”

Section: Diagnosismentioning

confidence: 99%

Musculoskeletal Pathology

Peat

Kawcak

2015

Veterinary Clinics of North America: Equine Practice

View full text Add to dashboard Cite

The current understanding of pathology as it relates to common diseases of the equine musculoskeletal system is reviewed. Conditions are organized under the fundamental categories of developmental, exercise-induced, infectious, and miscellaneous pathology. The overview of developmental pathology incorporates the new classification system of juvenile osteochondral conditions. Discussion of exercise-induced pathology emphasizes increased understanding of the contribution of cumulative microdamage caused by repetitive cyclic loading. Miscellaneous musculoskeletal pathology focuses on laminitis, which current knowledge indicates should be regarded as a clinical syndrome with a variety of possible distinct mechanisms of structural failure that are outlined in this overview.

show abstract

“…In the case of naturally occurring equine traumatic OA, the Fourier transform infrared spectroscopy (FTIR) approach has been confirmed as highly accurate for synovial fluid when compared to arthroscopy . The limitations of such studies are that they have been conducted on clinically apparent cases and have not been tested in a preclinical population of horses for which prospective synovial fluid analysis would be impractical …”

Section: Equine Musculoskeletal Biomarkers: Current Knowledge and Futmentioning

confidence: 99%

“…12 A clinical study in 238 racehorses, employing monthly musculoskeletal examinations and blood samples, showed that it was possible to correctly predict horses that would sustain an injury 74% of the time. 34 Recent work evaluating proteinases has shown that: (i) the presence of lumican and a 29 kD lumican catabolite increased with the onset and progression of OA 35,36 ; (ii) a splice variant of one of the aggrecanases (ADAMTS4) was identified that appears to be specifically synthesized by human OA synovium and is associated with aggrecan degradation in the superficial zone of articular cartilage 37 ; and (iii) synovial fluid ADAMTS4 activity is a marker of inflammation and effusion. 38 Such findings have biologic/disease rationale as confirmed by OA onset in a STR/ORT mouse model being significantly reduced using monoclonal antibodies directed against substrate recognition domains of ADAMTS5.…”

Section: Transcriptome Analyses Of Meniscus and Anterior Cruciate Ligmentioning

confidence: 99%

Biomarkers for equine joint injury and osteoarthritis

McIlwraith

Kawcak

Frisbie

et al. 2018

Journal Orthopaedic Research

View full text Add to dashboard Cite

We report the results of a symposium aimed at identifying validated biomarkers that can be used to complement clinical observations for diagnosis and prognosis of joint injury leading to equine osteoarthritis (OA). Biomarkers might also predict pre-fracture change that could lead to catastrophic bone failure in equine athletes. The workshop was attended by leading scientists in the fields of equine and human musculoskeletal biomarkers to enable cross-disciplinary exchange and improve knowledge in both. Detailed proceedings with strategic planning was written, added to, edited and referenced to develop this manuscript. The most recent information from work in equine and human osteoarthritic biomarkers was accumulated, including the use of personalized healthcare to stratify OA phenotypes, transcriptome analysis of anterior cruciate ligament (ACL) and meniscal injuries in the human knee. The spectrum of "wet" biomarker assays that are antibody based that have achieved usefulness in both humans and horses, imaging biomarkers and the role they can play in equine and human OA was discussed. Prediction of musculoskeletal injury in the horse remains a challenge, and the potential usefulness of spectroscopy, metabolomics, proteomics, and development of biobanks to classify biomarkers in different stages of equine and human OA were reviewed. The participants concluded that new information and studies in equine musculoskeletal biomarkers have potential translational value for humans and vice versa. OA is equally important in humans and horses, and the welfare issues associated with catastrophic musculoskeletal injury in horses add further emphasis to the need for good validated biomarkers in the horse. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:823-831, 2018.

show abstract

Serum biomarker levels for musculoskeletal disease in two‐ and three‐year‐old racing Thoroughbred horses: A prospective study of 130 horses

Abstract: Biomarkers have the potential to be used as a screening aid prior to musculoskeletal injury.

Cited by 38 publications

References 35 publications

Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise

Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise

Musculoskeletal Pathology

Biomarkers for equine joint injury and osteoarthritis

Contact Info

Product

Resources

About